A SNP molecular marker that can be traced back to the source of the double umbilical snail and its application

Abstract

The invention discloses a SNP molecular marker that can be used to trace the source of double umbilical snails and its application. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO.1, with a total of 857bp, and there is a base mutation 305G→305A at the 305th base, which is specifically recognized by the BstUI restriction endonuclease. By detecting and identifying the distribution of alleles of the SNP molecular marker in 90 umbilical snail test populations from 3 different locations, it was found that the allele frequencies of the SNP molecular marker in different locations were close to each other, and the polymorphism was abundant. The difference in allele frequency distribution is small, and the heterozygosity is greater than 0.3. It is preliminarily determined that the SNP molecular marker can be used as the DNA traceability of the double umbilical snail.

Description

technical field [0001] The invention belongs to the technical field of molecular detection. More specifically, it relates to a double umbilical snail SNP molecular marker and its application in traceability. Background technique [0002] Double-umbilical snails belong to the phylum Molluscs, Pneumonia subclass, and Snail family. Among the more than 30 known species of double-umbilical snails, double-umbilical snails smooth, double-umbilical snails, shoal double-umbilical snails, and Puer's double-umbilical snails 18 species including S. mansoni and other 18 species can act as intermediate hosts of Schistosoma mansoni to spread schistosomiasis, causing serious disease burden to the world, especially in Africa, South America, Asia and other regions, and seriously hindering social and economic development. Among them, the double umbilical snails are mainly distributed in the fresh waters of St. Lucie in the Caribbean Sea and the northeastern coast of Brazil. They are highly su...

AI Technical Summary

Problems solved by technology

[0004] AFLP marker is the first generation of molecular marker method, which has rich polymorphism, strong genetic stability and high reliability, but it has high requirements on the quality and purity of DNA template and the level of operators, and it is difficult to count. The cost is relatively high; SSR marker is the second generation molecular marker method, which has the advantages of high polymorphism, low mutation rate and less template required, but the band pattern of the marker is complicated, the process is cumbersome, and it is not suitable for automatic typing ; and SNP, as a third-generation molecular marker, refers to the variation of a single nucleotide at the same site in the genome. It has the advantages of wide distribution, high density, strong representativeness, and good genetic stability. It does not require high template quality. Generally manifested as two alleles, showing either-or characteristics, easy to determine the type, and suitable for high-throughput automatic detection and typing, it is currently the most effective method for traceability molecular markers

[0005] However, the SNP molecular marker used for traceability is different from general SNP molecular markers, and it must have at least the following characteristics: (1) high variability and close allele frequencies; (2) small differences in allele distribution frequencies between populations; (3) The degree of heterozygosity is greater than 0.3

Regarding traceable SNP molecular markers, patent CN201710607015.8 discloses a SNP marker combination and its application for traceability identification of beef cattle and meat products, and patents CN201510785854. SNP molecular markers used for traceability on chromosome No. 2 and their applications; patent CN201210529628.1 discloses a set of geographically specific Plasmodium vivax molecular markers and their application in strain traceability; Related reports on the SNP sites of snail traceability

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