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New short nucleotide tandem repeat site and application thereof

A short tandem repeat and nucleotide sequence technology, applied in the field of short nucleotide tandem repeat sequence sites, can solve the problems of poor compatibility and achieve high compatibility, high heterozygosity, and high resolution

Active Publication Date: 2014-10-15
GENESKY DIAGNOSTICS SUZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, in order to overcome defects such as poor compatibility, presence of CNV or location in highly repetitive sequence regions, a new type of short tandem repeat sequence with high discrimination is still needed in this field

Method used

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  • New short nucleotide tandem repeat site and application thereof
  • New short nucleotide tandem repeat site and application thereof
  • New short nucleotide tandem repeat site and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0154] Combine the new STR locus G7S0005 of the present invention with 10 commonly used gene loci (VWA, D16S539, D5S818, D7S820, D13S317, D8S1179, D18S51, TH01, D2S1338, AMELO), and use multiple fluorescent PCR technology to check the samples, 4 used The samples were a sample of a family of three with a relationship, and a control sample with no relationship. The primer sequences of G7S0005 and 10 commonly used loci are shown in Table 2.

[0155] Table 2 Primer sequence

[0156]

[0157] Note: Four fluorescent dyes: PET, VIC, NED, and FAM (Applied Biosystems, USA).

[0158]

[0159] The specific experimental operation steps are as follows:

[0160] 1) DNA sample preparation

[0161] Collect blood from 4 selected individual samples; extract DNA samples with DNA extraction kit and take 1μl each, perform quality inspection and concentration estimation of the samples with 1% agarose electrophoresis, and then dilute the samples to working concentration according to the estimated concentrati...

Embodiment 2

[0175] Twenty groups of samples with and without genetic relationship were randomly selected, and the method of Example 1 was used for detection, except that only the new STR locus G7S0005 of the present invention was used.

[0176] After testing, after determining the paternal samples, determine the offspring samples of the remaining 19 groups of samples that are related to the paternal parent. The new STR locus G7S0005 of the present invention can accurately exclude the 6 groups of unrelated samples. A high degree of discrimination.

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Abstract

The invention discloses a new short nucleotide tandem repeat site and application thereof. A short tandem repeat locus G7S0005 has a sequence shown as SEQ ID NO.1, and can be used for preparation of (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity testing or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and / or (e) kits for detecting whether leucocytes in a bone marrow transplantation receptor are substituted by donor cells. The short tandem repeat locus G7S0005 has high discrimination, and can effectively analyze the genetic relationship.

Description

[0001] technical field [0002] The invention relates to a short nucleotide tandem repeat sequence site and its application. [0003] Background technique [0004] Short tandem repeat (Short Tandem Repeat. STR), also known as microsatellite DNA (microsatellite DNA), is a kind of DNA tandem repeat sequence with a repeat unit of 2-6bp, a repeat number of 10-60, and a fragment length of less than 400bp; The reason is slippage during DNA replication, or base mismatch between the slipping strand and the complementary strand during replication and repair, resulting in the deletion or insertion of one or several repeat units. STR has a wide distribution in the genome, multiple alleles, high heterozygosity, and stable typing results. STR inheritance conforms to Mendelian inheritance laws (Koreth, J., et al. 1996. Microsatellites and PCR ge-nomic analysis.J Pathol.178: 239-248.) and other characteristics, and when multiple STR loci are jointly detected, the individual recognition a...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 姜正文马瑞晓张希
Owner GENESKY DIAGNOSTICS SUZHOU
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