Nucleic acid mass spectrometry paternity testing method based on information SNP (Single Nucleotide Polymorphism) set and primers of information SNP set

Abstract

The invention discloses a nucleic acid mass spectrometry paternity testing method based on an information SNP (Single Nucleotide Polymorphism) set and primers of information SNP set. The information SNP set used in the method which is a data analysis based on forty groups of people has a higher heterozygous rate greater than 0.4, a population genetic index Fst less than 0.06, lower mutation frequency less than 0.01 percent and a random population matching rate less than 10<-15>, and is suitable for individual identification and paternity identification, wherein the nucleotide sequences of thirty information SNP-marked sense primers are sequentially shown as SEQ ID NO.1-30, the nucleotide sequences of antisense primers are sequentially shown as SEQ ID NO.31-60, and the nucleotide sequencesof single-base extension primers are sequentially shown as SEQ ID NO.61-90. Verified by a flight mass spectrometry information SNP polymorphism and paternity inference experiment, a testing result matches an actual genetic relationship. When the information SNP set provided by the invention is used for human parentage testing, results are accurate, the mass spectrometry method is preferred to be utilized, genetic typing can be quickly and conveniently carried out at high throughput, and thereby a parentage index is calculated and paternity judgment is carried out. The method has the advantagesof high accuracy, low cost and the like.

Description

technical field [0001] The invention relates to a detection system for human paternity identification by using nucleic acid mass spectrometry to detect information SNP markers, in particular to 30 information SNP marker combinations, primer sequences and a parentage identification method. Background technique [0002] Parentage Testing is the identification of the blood relationship between individuals through the detection of human genetic markers and the analysis of genetic laws. Modern paternity testing methods use DNA analysis, currently mainly based on capillary electrophoresis (Capillary Electrophoresis, CE) PCR-STR composite fluorescence amplification detection, which is the application of the second-generation genetic marker short tandem repeat (STR) typing technology , that is, through the detection, typing and statistics of 15-20 STRs on DNA samples to achieve the purpose of calculating the probability of parentage. The STR locus is considered to be the most versa...

AI Technical Summary

Problems solved by technology

However, with the wide application of STR loci in forensic identification, its defects are becoming more and more prominent, such as: the high mutation rate of STR loci brings troubles to the interpretation of paternity test results; PCR amplification is not easy to achieve and prevents degradation DNA typing; the limited number of STRs is not conducive to the identification of complex genetic relationships

In addition, because the sequence lengths of different alleles of STR are similar, this will lead to a high error rate of 1%-5%, which will directly affect the interpretation of paternity test results

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