A method and application of microsatellite identification and division of Hu sheep family

A microsatellite and microsatellite marker technology, which is applied in the field of molecular markers of animal genetics and breeding, can solve the problems of few reports on the identification and division of Hu sheep families, and solve the problems of Hu sheep family confusion, high heterozygosity and allelicity. The effect of a large number of genes

Active Publication Date: 2021-01-05
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0004] At present, there are few reports on the identification and division of Hu sheep family

Method used

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  • A method and application of microsatellite identification and division of Hu sheep family
  • A method and application of microsatellite identification and division of Hu sheep family
  • A method and application of microsatellite identification and division of Hu sheep family

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Embodiment 1

[0024] (1) Extract the DNA of Hu sheep from the family to be identified

[0025] Select 30 Hu sheep rams to be tested, collect 1-2 mL of blood from the jugular vein, store in EDTA-containing blood collection tubes for later use, and use the TIANGEN Blood Genomic DNA Extraction Kit to extract the genomic DNA of the samples, and the extraction steps refer to the product manual (http: / / www.tiangen.com / asset / imsupload / up0406252001543631420.pdf).

[0026] (2) Selection and reaction system of polymorphic microsatellite markers in Hu sheep

[0027] According to the published literature (Zeng Jianhua et al. "Evaluation of the Conservation Effect of Hu Sheep Resources Based on Microsatellite Markers") and the sheep microsatellite loci recommended by the International Society of Animal Genetics (https: / / www.isag.us / ), screening Nine microsatellite loci were distributed on multiple autosomes, with low linkage, large number of alleles, high polymorphism and heterozygosity. The 5' ends ...

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Abstract

The invention discloses a method for identifying and dividing Hu sheep families by using microsatellites, and applications thereof. The method comprises the following steps: extracting Hu sheep genomeDNA; selecting a Hu sheep polymorphism microsatellite marker, and modifying with a fluorophore; carrying out landing type PCR amplification; and analyzing data such as genotypes and the like, identifying and dividing families, and carrying out other steps. According to the invention, a plurality of microsatellite sites are selected, are distributed in a plurality of autosomes, and are low in linkage, large in the number of alleles, and high in polymorphism and heterozygosity; the landing PCR amplification is utilized, so that the operation is convenient, the product specificity is strong, andthe Hu sheep family can be quickly and accurately identified and divided; and the accumulated single parent exclusion rate, the paternity exclusion rate and the amphiphilic exclusion rate reach 99.6072%, 99.8808% and 99.9999% respectively, the requirements of paternity test and pedigree analysis in genetic breeding can be completely met, and the method has great application value in Hu sheep family breeding and breeding.

Description

technical field [0001] The invention relates to the technical field of molecular markers in animal genetic breeding, in particular to a method for identifying and dividing Hu sheep families by using microsatellites and an application thereof. Background technique [0002] Hu sheep is a unique breed of sheep in China. It was originally produced in Jiaxing, Zhejiang and the Taihu Lake basin. It is the first-class protected local livestock and poultry species in my country. In recent years, breeding Hu sheep has become a key project for poverty alleviation, and the development of Hu sheep industry is of great significance to my country's animal husbandry. [0003] In sheep breeding, accurate pedigree information is of great significance for estimating genetic parameters, formulating breeding programs and avoiding inbreeding depression. As the excellent potential of Hu sheep is gradually discovered, its breeding scale is expanding year by year. However, most Hu sheep farms stil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11G16B20/30
CPCC12Q1/6858C12Q1/6888C12Q2600/156G16B20/30C12Q2531/113C12Q2563/107
Inventor 张莉赵志达王欣悦胡文萍侯浩宾
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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