Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method and application of microsatellite identification and division of Hu sheep family

A microsatellite and microsatellite marker technology, which is applied in the field of molecular markers of animal genetics and breeding, can solve the problems of few reports on the identification and division of Hu sheep families, and solve the problems of Hu sheep family confusion, high heterozygosity and allelicity. The effect of a large number of genes

Active Publication Date: 2021-01-05
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports on the identification and division of Hu sheep family

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method and application of microsatellite identification and division of Hu sheep family
  • A method and application of microsatellite identification and division of Hu sheep family
  • A method and application of microsatellite identification and division of Hu sheep family

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Extract the DNA of Hu sheep from the family to be identified

[0025] Select 30 Hu sheep rams to be tested, collect 1-2 mL of blood from the jugular vein, store in EDTA-containing blood collection tubes for later use, and use the TIANGEN Blood Genomic DNA Extraction Kit to extract the genomic DNA of the samples, and the extraction steps refer to the product manual (http: / / www.tiangen.com / asset / imsupload / up0406252001543631420.pdf).

[0026] (2) Selection and reaction system of polymorphic microsatellite markers in Hu sheep

[0027] According to the published literature (Zeng Jianhua et al. "Evaluation of the Conservation Effect of Hu Sheep Resources Based on Microsatellite Markers") and the sheep microsatellite loci recommended by the International Society of Animal Genetics (https: / / www.isag.us / ), screening Nine microsatellite loci were distributed on multiple autosomes, with low linkage, large number of alleles, high polymorphism and heterozygosity. The 5' ends ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying and dividing Hu sheep families by using microsatellites, and applications thereof. The method comprises the following steps: extracting Hu sheep genomeDNA; selecting a Hu sheep polymorphism microsatellite marker, and modifying with a fluorophore; carrying out landing type PCR amplification; and analyzing data such as genotypes and the like, identifying and dividing families, and carrying out other steps. According to the invention, a plurality of microsatellite sites are selected, are distributed in a plurality of autosomes, and are low in linkage, large in the number of alleles, and high in polymorphism and heterozygosity; the landing PCR amplification is utilized, so that the operation is convenient, the product specificity is strong, andthe Hu sheep family can be quickly and accurately identified and divided; and the accumulated single parent exclusion rate, the paternity exclusion rate and the amphiphilic exclusion rate reach 99.6072%, 99.8808% and 99.9999% respectively, the requirements of paternity test and pedigree analysis in genetic breeding can be completely met, and the method has great application value in Hu sheep family breeding and breeding.

Description

technical field [0001] The invention relates to the technical field of molecular markers in animal genetic breeding, in particular to a method for identifying and dividing Hu sheep families by using microsatellites and an application thereof. Background technique [0002] Hu sheep is a unique breed of sheep in China. It was originally produced in Jiaxing, Zhejiang and the Taihu Lake basin. It is the first-class protected local livestock and poultry species in my country. In recent years, breeding Hu sheep has become a key project for poverty alleviation, and the development of Hu sheep industry is of great significance to my country's animal husbandry. [0003] In sheep breeding, accurate pedigree information is of great significance for estimating genetic parameters, formulating breeding programs and avoiding inbreeding depression. As the excellent potential of Hu sheep is gradually discovered, its breeding scale is expanding year by year. However, most Hu sheep farms stil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11G16B20/30
CPCC12Q1/6858C12Q1/6888C12Q2600/156G16B20/30C12Q2531/113C12Q2563/107
Inventor 张莉赵志达王欣悦胡文萍侯浩宾
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com