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Microsatellite marker primers for identification of microsatellite family of Penaeus monodon, identification method and application

A technology of microsatellite markers and Penaeus monodon, which is applied in the field of molecular markers in shrimp genetic breeding, can solve the problems of few reports on identification studies, and achieve the effects of saving experimental costs, high polymorphism, and improving efficiency

Active Publication Date: 2019-01-11
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there are few reports on the application of microsatellite markers in the family identification of Penaeus monodon

Method used

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  • Microsatellite marker primers for identification of microsatellite family of Penaeus monodon, identification method and application
  • Microsatellite marker primers for identification of microsatellite family of Penaeus monodon, identification method and application
  • Microsatellite marker primers for identification of microsatellite family of Penaeus monodon, identification method and application

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Experimental program
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Effect test

Embodiment 1

[0043] Example 1 Screening and Synthesis of Microsatellite Marker Primers for Microsatellite Family Identification of Penaeus monodon

[0044] Primers were designed by selecting sequences containing microsatellite markers from the transcriptome library of Penaeus monodon, and the unigene containing microsatellite repeat sequences of Penaeus monodon was screened by transcriptome sequencing, and then the unigene containing microsatellite markers was detected from the transcriptome library of Penaeus monodon Design microsatellite-specific primers for the sequence of satellite loci to detect their polymorphisms. After screening, 6 pairs of primers with clear bands and high polymorphisms were finally selected as primers for family identification. Ltd Synthetics.

[0045]The microsatellite marker primers selected in the present invention are rich in polymorphism, suitable for fragment size intervals and non-linked between markers, and are easy to type and detect at the same time, an...

Embodiment 2

[0048] The identification method of embodiment 2 Penaeus monodon microsatellite families

[0049] (1) DNA extraction from the parents and offspring of Penaeus monodon

[0050] (1) Establishment of the family of Penaeus monodon

[0051] Breeding new species of Penaeus monodon "Nanhai No. 1" and 10 females and 10 males and females were selected from the wild population in Thailand, and artificially propagated. The ratio of male to female is 1:1, and 10 full-sibling families were established, and the muscle tissue of the parents of each family was cut. Put them in absolute ethanol, and record the family information, store them at -20°C, put the 10 families in different ponds for breeding, after half a year, randomly select 10 shrimps from each family, and take the muscle Fix with absolute ethanol as a sample for family identification.

[0052] (2) Extraction of Genomic DNA from Penaeus monodon parents and offspring

[0053] Using the Magen Animal Tissue DNA Extraction Kit, pro...

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Abstract

The invention discloses microsatellite marker primers used for penaeus monodon microsatellite family identification. The microsatellite marker primers include six primer pairs in total, and the six primer pairs are PM-38, PM-69, PM-92, PM-84, PM-102 and PM-114 respectively. The invention further discloses an identification method for the penaeus monodon microsatellite family and application of the microsatellite marker primers to penaeus monodon microsatellite family identification. By the adoption of the microsatellite marker primers, a paternity test platform is set up on penaeus monodon with polymorphic microsatellite markers marked by fluorescence for the first time, and the identification accuracy reaches 99%; different families and sources of the penaeus monodon can be effectively and fast identified, and a basis is provided for breeding, reproduction matching and enhancement and releasing assessment of the penaeus monodon.

Description

technical field [0001] The invention belongs to the technical field of molecular markers for shrimp genetic breeding, and in particular relates to a microsatellite marker primer for identification of microsatellite family of Penaeus monodon, identification method and application. Background technique [0002] Penaeus monodon (Penaeus monodon) is the largest individual prawn species. It is widely distributed in most sea areas of the Indian Ocean and the Western Pacific Ocean. It is one of the most important farmed prawns in the world. Important breeding objects in coastal provinces. [0003] Most of the cultured seedlings of Penaeus monodon are obtained through artificial propagation, and only a few are obtained from sea catches. Broodstock for artificial breeding mainly comes from two aspects: one is to catch wild brood shrimp, and the other is to remove eyestalks and artificially induce wild female shrimp to mature and lay eggs, but both rely on wild resources. In recent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2600/156C12Q2563/107
Inventor 张殿昌朱克诚江世贵张楠郭华阳
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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