19 results about "Penaeus kerathurus" patented technology

The invention discloses a penaeus vannamei body size estimation method and device and a storage medium, and relates to the technical field of breeding industry image processing, and the method comprises the steps: collecting a penaeus vannamei data set image, and carrying out the marking of the penaeus vannamei data set image; constructing a penaeus vannamei body detection model, improving the penaeus vannamei body detection model, and training the improved penaeus vannamei body detection model based on the marked penaeus vannamei body data set image; and based on the trained penaeus vannamei body detection model, measuring the body size of the penaeus vannamei and estimating the length of the penaeus vannamei body. According to the method, the expression and fusion capabilities of the multi-scale features are enhanced, the positioning precision of the feature points of the prawns is improved, and a set of non-contact estimation system for the body sizes of the prawns is developed. The coordinates of the feature points of the shrimp bodies are accurately obtained, the body size indexes are calculated, and accurate estimation of the shrimps with different completeness degrees is achieved. Rapid and accurate non-contact prawn body size measurement is realized, and powerful technical support is provided for intelligent and accurate cultivation of the penaeus vannamei.

The invention discloses a probe for detecting a penaeus japonicus SNP molecular marker combination, a penaeus japonicus 31K liquid phase breeding chip and application, and belongs to the technical field of molecular marker breeding. The penaeus japonicus SNP molecular marker combination is composed of 31097 SNP molecular markers. The 31097 SNP molecular markers are as shown in the specification table 1. The invention also provides application of the penaeus japonicus liquid phase breeding chip in penaeus japonicus breeding, sample detection, genetic resource genetic diversity analysis, genetic and evolutionary analysis, genetic relationship identification, whole genome association analysis and penaeus japonicus genome selection. The penaeus japonicus 31K liquid phase breeding chip provided by the invention has the characteristics of good site representativeness, strong specificity, high polymorphism and the like, is widely applicable to detection of different varieties of penaeus japonicus materials, can establish a modern breeding system, and promotes the development of the penaeus japonicus molecular breeding industry.

The application is a deep learning-based method for breeding and multiplying Penaeus vannamei, which comprises: obtaining genomic data of the initial population; obtaining specific trait data of the screened initial population; locating a specific gene sequence set according to the specific trait data and the genomic data; the gene sequence set is a gene number set related to the specific trait; and determining the breeding shrimp for breeding according to the gene number set. The application first breeds Penaeus vannamei populations of different families, obtains the trait phenotype data and genomic data of Penaeus vannamei under different families, determines the gene sequence meeting the trait phenotype requirements according to the correlation degree between the trait phenotype data and the genomic data, that is, locates the gene sequence related to the specific trait, and then selects the next generation of Penaeus vannamei according to the gene sequence set, or directly breeds the breeding shrimp with stable traits.

The invention discloses a penaeus monodon NFAT5 gene and application thereof, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the NFAT5 gene is as shown in SEQ ID NO: 2. The invention further discloses a recombinant expression vector containing the penaeus monodon NFAT5 gene, a host cell containing the recombinant expression vector, and an application of the NFAT5 gene. The invention further discloses application of the NFAT5 gene of the penaeus monodon, the protein, the recombinant expression vector containing the gene or the host cell containing the expression vector to preparation of a gene preparation or a feed additive for improving the low-salt tolerance of the penaeus monodon.

The invention discloses a penaeus monodon DNA (deoxyribonucleic acid) fingerprint spectrum which comprises at least 209 specific SNP (single nucleotide polymorphism) sites and realizes efficient and accurate distinguishing of different culture populations, so that technical guarantee is provided for identification and traceability work of penaeus monodon germplasm, and meanwhile, an accurate molecular technology platform for germplasm resource development, utilization and protection is built. The invention further discloses a construction method of the DNA fingerprint spectrum of the penaeus monodon and application of the DNA fingerprint spectrum of the penaeus monodon in identification of germplasm resources of the penaeus monodon and assistant breeding.

This invention discloses a Penaeus monodon NFAT5 gene and its application. The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the amino acid sequence of the protein encoded by the NFAT5 gene is shown in SEQ ID NO:2. This invention also discloses a recombinant expression vector containing the Penaeus monodon NFAT5 gene, a host cell containing the recombinant expression vector, and the use of the Penaeus monodon NFAT5 gene, protein, recombinant expression vector containing the gene, or host cell containing the expression vector in the preparation of gene preparations or feed additives for improving the low salt tolerance of Penaeus monodon.

The invention belongs to the technical field of biological agents, and discloses a biological agent for preventing and treating enteritis diseases of penaeus vannamei as well as a preparation method and application of the biological agent, the biological agent comprises lactobacillus rhamnosus BY13207, and the total viable count of the biological agent is 1.5 * 10 < 9 >-3 * 10 < 9 > cfu / mL; the lactobacillus rhamnosus BY13207 is preserved in the China General Microbiological Culture Collection Center on August 11, 2025, and the preservation number of the lactobacillus rhamnosus BY13207 is CGMCC No.35559. The lactobacillus rhamnosus BY13207 added in the lactobacillus rhamnosus BY13207 has very strong antagonistic action and anti-inflammatory action, can inhibit the growth and reproduction of various pathogenic microorganisms of prawns and enhance the disease resistance of the prawns; the compound has broad-spectrum antibacterial activity, and can effectively eliminate harmful bacteria in prawn culture water and reduce the risk of disease occurrence.

The invention discloses a litopenaeus vannamei MAPT-LIKE gene, an SNP (Single Nucleotide Polymorphism) marker, a detection primer, a kit and application of the litopenaeus vannamei MAPT-LIKE gene. The sequence of the SNP marker is as shown in SEQ ID NO.1, the SNP site is located at the 554th site from the 5'end of the SEQ ID NO.1, and the polymorphic form is C / T type; the SNP locus can be used for breeding litopenaeus vannamei through body length or growth rate screening, and a primer designed according to the SEQ ID NO.1 sequence or a kit prepared from the primer can be used for detecting the SNP locus. According to the SNP marker Chr3541822951, parents can be selected according to the CC genotype, the body length growth speed of breeding populations can be increased, an innovative tool is provided for molecular breeding of litopenaeus vannamei, the breeding period is shortened, and industrial development and efficiency improvement are assisted.

The application discloses a high-activity penaeus vannamei breeding pond capable of automatically adjusting breeding density and relates to the technical field of the penaeus vannamei breeding pond. The high-activity penaeus vannamei breeding pond comprises a water storage pond, a fixed frame fixedly connected to the upper portion of the water storage pond, a motor installed on the fixed frame, and a water supply pipe fixedly connected to the upper surface of the water storage pond. The high-activity penaeus vannamei breeding pond comprises an automatic pollution discharge mechanism, an automatic water supply mechanism and an automatic feeding mechanism. The automatic pollution discharge mechanism is arranged at the middle portion of the water storage pond, the automatic water supply mechanism is arranged on the upper surface of the water storage pond, and the automatic feeding mechanism is arranged on the fixed frame. The automatic pollution discharge mechanism is arranged, and automatic quantitative and timed feeding of the feed can ensure that the penaeus vannamei uniformly obtains the feed in the breeding pond, can avoid that some penaeus vannamei obtain insufficient food and nutrition, can ensure the growth and development of the whole breeding population, can fully meet the demand of the penaeus vannamei in each feeding, can reduce the problems of excessive feeding and bait waste, and can save the breeding cost.

The present application relates to a kind of penaeus vannamei ovipositor breeding ponds, including several ovipositor breeding pond bodies, the upper portion in ovipositor breeding pond body is equipped with for the reproduction of seed shrimp spawning space, the lower portion of spawning space is equipped with for the hatching of shrimp eggs ovipositor space, the bottom of ovipositor space is equipped with one side with pool wall form the ovipositor bottom plate of positionable hinged, ovipositor bottom plate includes upper portion of egg-blocking net layer, middle portion of aeration layer, lower portion of water-permeable net layer, aeration layer is equipped with multiple aeration pipes, aeration pipe is communicated with external air source, aeration pipe is distributed with aeration hole, the lower portion of ovipositor space is equipped with for the cultivation of fry breeding space, the bottom of breeding space is equipped with fry outlet on one side.The present application uses the way of pond bottom aeration to replace the existing artificial ovipositor and mechanism ovipositor mode, integrates the hatching and fry function of prawn on the ovipositor breeding pond body, with better ovipositor effect, breeding yield is improved, breeding cost is reduced, and ovipositor breeding efficiency is significantly improved, etc.

This patent discloses a cryopreservation solution and method for improving the sperm survival rate of Penaeus monodon, comprising a base solution and combined cryoprotectants. The base solution is a Penaeus monodon sperm cell culture medium containing Penaeus monodon serum; the combined cryoprotectants include permeable and non-permeable cryoprotectants. By using Penaeus monodon sperm cell culture medium instead of traditional calcium-free saline as the base solution for cryopreservation, an optimized environmental basis is established for cryopreservation, significantly improving cryoprotection efficiency. The cryopreservation method provided in this application enables Penaeus monodon sperm to achieve a survival rate of over 89% after 10 months of cryopreservation, achieving long-term stable preservation of sperm under ultra-low temperature conditions; its key biological activity indicators show no significant difference compared to before cryopreservation, successfully achieving artificial insemination. This provides key technical support for the long-term preservation of Penaeus monodon germplasm resources and lays the foundation for the construction of germplasm banks.

This invention relates to the field of aquaculture technology, specifically a feed formulation and preparation method for improving the reproductive performance of broodstock tiger prawns. The method includes: determining the specific nutritional requirements of broodstock tiger prawns during gonadal development; screening core animal and plant protein sources and determining their basic mass ratios accordingly; performing targeted biological treatment on the core raw materials to enrich gonadotropin-active small molecules; compounding these with phospholipids, attractants, vitamins, and minerals to form a functional additive combination; mixing the core raw materials and additive combination in proportion, granulating, and adding antioxidants during processing; and finally verifying the formulation effect and confirming the final compound feed through standardized aquaculture trials. The compound feed obtained based on this method can effectively replace traditional live feed, improve broodstock tiger spawning, hatching rate, and larval quality, and has advantages such as balanced nutrition, stable quality, and suitability for large-scale production, solving the industry problems of live feed being prone to pathogens, supply fluctuations, and high costs.

This invention discloses a molecular marker 03W4 for high WSSV resistance in Litopenaeus vannamei and its applications, belonging to the field of molecular marker-assisted breeding technology for aquatic animals. The nucleotide sequence of the molecular marker 03W4 is shown in SEQ ID No. 1, and the genotype TT at the 301st locus of the molecular marker 03W4 is a WSSV resistance genotype. This invention also provides primers for the molecular marker 03W4, including the amplification primers shown in SEQ ID No. 2 and SEQ ID No. 3, and the extension primer shown in SEQ ID No. 4. The molecular marker 03W4 for high WSSV resistance in Litopenaeus vannamei can be used for early selection of Litopenaeus vannamei regardless of growth stage, screening for highly WSSV-resistant individuals in breeding populations with different growth performances, rapidly selecting highly WSSV-resistant parent populations, and promoting the breeding process of new Litopenaeus vannamei varieties with high WSSV resistance.

The application relates to the field of aquaculture, in particular to a culture method of longsnout catfish (Leiocassis longirostris) in the culture of penaeus vannamei, which comprises the following steps: pond preparation, fry stocking, feed feeding, water quality management, daily management, fishing and harvesting, etc. The application establishes a multi-nutrient level culture mode of fish and shrimp symbiosis, fully utilizes the water space of the culture pond of penaeus vannamei, improves the water utilization rate of the pond, improves the production efficiency, utilizes the habits of longsnout catfish and penaeus vannamei, plays the effect of fish and shrimp ecological comprehensive disease prevention, improves the growth environment of fish, improves the feed utilization rate, reduces the total feed coefficient, reduces the wastewater discharge, and improves the energy efficiency of aquaculture. The application is simple in operation and easy to popularize.

The application discloses a Bacillus altitudinis JYD51 and application thereof in aquaculture, which has been preserved in the China General Microbiological Culture Collection Center on October 10, 2024, and the preservation number is CGMCC No.32164. The JYD51 strain can be fermented by using a culture medium rich in organic matter, and the fermentation product has no toxic effect on Penaeus vannamei and crabs, can inhibit the infection of hepatopancreas ciliates on the Penaeus vannamei and the harm of Paramoeba on the crabs. Meanwhile, the microbial agent prepared by the JYD51 strain and Bacillus sphaericus JYQ7 can effectively control the harm of the hepatopancreas ciliates on the Penaeus vannamei and the Paramoeba on the crabs, purify the water environment for the growth of the Penaeus vannamei and the crabs, and improve the survival rate and product quality of the Penaeus vannamei and the crabs in aquaculture.

The application discloses a BBOX1 gene molecular marker related to the Vibrio resistance trait of Penaeus vannamei, which comprises any one or more of a molecular marker A, a molecular marker B and a molecular marker C, and the molecular markers are located on a gamma-butyrobetaine dioxygenase gene sequence of the Penaeus vannamei; the molecular marker is used as a functional marker of the Vibrio resistance trait of the Penaeus vannamei, the genomic DNA of muscle tissue of the Penaeus vannamei to be detected is extracted, and then the genomic DNA is used as template DNA to perform PCR amplification and purification of a PCR amplification product, and then the product is sequenced to determine the genotypes of the molecular marker A, the molecular marker B and the molecular marker C, so that the genotypic individuals with the Vibrio resistance advantage are screened to breed the Penaeus vannamei, the obtained result is accurate and has high application value, the breeding process of the fine variety with the Vibrio resistance trait can be accelerated, and the survival rate of the shrimp culture is improved, which has important significance.

The present invention relates to a feed composition suitable for feeding crustaceans comprising mannose oligosaccharides. The invention also relates to the use of mannose oligosaccharide compositions for feeding crustaceans. The invention also relates to crustaceans as a food source, in particular shrimp of the species Litopenaeus vannamei or Penaeus monodon, fed with the feed composition comprising mannose oligosaccharides.

The application discloses a BHMT gene molecular marker related to Litopenaeus vannamei resistance to hepatopancreatic intestinal coccidiosis and a detection primer and application thereof, and has the characteristics that the molecular marker comprises three SNP sites on the BHMT gene; the nucleotide sequence of the molecular marker A is shown in SEQ ID NO. 1, the mutation type at the 252th base is T>A, the nucleotide sequence of the molecular marker B is shown in SEQ ID NO. 2, the mutation type at the 93th base is C>T, and the nucleotide sequence of the molecular marker C is shown in SEQ ID NO. 2, and the mutation type at the 636th base is G>A; the parent shrimp with the TA genotype of the BHMT-8159 site, the CT genotype of the BHMT-12167 site and the GG genotype of the BHMT-12710 site is selected as the parent shrimp resistant to hepatopancreatic intestinal coccidiosis; and the application has the advantages of good breeding efficiency and accuracy.

The invention belongs to the technical field of biology, and particularly relates to an efficient prawn DNA mixed pool construction method and breeding application. The method specifically comprises the following steps: taking prawn tails of prawns as tissues of equivalent mixed single individuals, taking one prawn tails of each to-be-treated individual, merging and mixing the taken prawn tails, cutting the same length to realize uniform mixing of the equivalent tissues of the individuals, and then extracting DNA of the mixed tissues, namely constructing a DNA mixed pool. The technical scheme provided by the invention effectively solves the problems of difficulty in accurate quantification, complex operation and high cost in construction of a large number of DNA mixed pool tissues in traditional BSA analysis, simplifies the identification process of the character-related markers, is suitable for genetic analysis of large-scale prawn samples and identification of the character-related markers, and has a wide application prospect in the field of prawn breeding.