112 results about "Penaeus kerathurus" patented technology

The invention discloses compound feed special for penaeus monodon breeding season nutrient enrichment and a preparation method thereof. The compound feed comprises Peru fish powder, sleeve-fish meat powder, shrimp meat powder, pork liver powder and the like, can replace conventional fresh baits, solves the problems that source and quality of the fresh baits are unstable, also can promote gonad development of parental shrimps, and improves egg laying amount, egg hatchability and larva abnormal rate of penaeus monodon parental shrimps; spread of viral diseases among penaeus monodon is reduced, production of healthy penaeus monodon larvae is ensured, and stability of the compound feed under water is good, the compound feed is easy for the parental shrimps to absorb, water environment pollution in aquiculture areas is reduced, and therefore the compound feed has popularization and application value.

The invention discloses microsatellite marker primers used for penaeus monodon microsatellite family identification. The microsatellite marker primers include six primer pairs in total, and the six primer pairs are PM-38, PM-69, PM-92, PM-84, PM-102 and PM-114 respectively. The invention further discloses an identification method for the penaeus monodon microsatellite family and application of the microsatellite marker primers to penaeus monodon microsatellite family identification. By the adoption of the microsatellite marker primers, a paternity test platform is set up on penaeus monodon with polymorphic microsatellite markers marked by fluorescence for the first time, and the identification accuracy reaches 99%; different families and sources of the penaeus monodon can be effectively and fast identified, and a basis is provided for breeding, reproduction matching and enhancement and releasing assessment of the penaeus monodon.

The invention relates to the technical field of aquaculture, in particular to a prawn aquaculture pond. The prawn aquaculture pond comprises an aquaculture pond and a sedimentation tank, an inclined sewage collection plate and a collection tank are arranged at the bottom of the aquaculture pond, and a water pumping pipe is arranged at the horizontal lowest position of the collection tank. More than two conical sedimentation plates are peripherally connected to the horizontal section of the side wall of the sedimentation tank, a shell is provided with a cyclic water outlet above the uppermost sedimentation plates in the shell, and the cyclic water outlet is connected with the aquaculture pond through a pipeline. The center of the bottom wall is provided with a sewage outlet which is connected with a sewage outlet pipeline. The prawn aquaculture pond has advantages that by the sedimentation tank for cyclic purification of water in the aquaculture pond, cyclic treatment of aquaculture wastewater is completed so as to realize environment friendliness in aquaculture, and aquaculture yield can be increased; by an aquaculture system, water replacement amount is greatly decreased, water source and electric energy are saved, and accordingly aquaculture cost is greatly reduced.

The invention discloses a jellyfish breeding method including subjecting jellyfishes to mixed culture with ruditapes philippinarum, sinonovacula constrictas, penaeus chinensis, penaeus monodons and goby; controlling ecological breeding technologies including a stocking system based on breeding species and density, a feeding system based on a match between the species and number and a water quality control system based on a principle that firstly applying fertilizer into water, secondly maintaining fertilizer content and thirdly controlling the water quality. In this way, the jellyfishes and other species are rapid to grow and high in output and economic benefit. By the use of the jellyfish breeding method, the jellyfish output reaches 200-400kg/mu, the outputs of the ruditapes philippinarum and the sinonovacula constrictas reach 200-300kg/mu, and the outputs of the penaeus chinensis and the penaeus monodons reach 10-30kg/mu, and the goby output reaches 20-30kg/mu.

The invention relates to an anti-stress feed for culturing Penaeus vannamei Boone in a greenhouse. The anti-stress feed comprises 10-50% of fish meal, 5-10% of chicken powder, 3-10% of shrimp powder, 3-10% of a beer yeast, 10-30% of fermented soybean meal, 5-10% of squid powder, 10-16% of flour, 1-5% of fish oil, 1-3% of soybean phosphatide, 1-3% of betaine, 1-3% of a vitamin mixture, 2-4% of a mineral element mixture, 0.1-0.5% of Bacillus subtilis and 0.2-1% of taurine. According to the invention, the anti-stress feed selects feed raw materials having the advantages of high digestion and absorption utilization rate and strong food attraction against the growth and physiological digestion characteristics of the Penaeus vannamei Boone in the process of culturing in the greenhouse, trace nutritional elements comprising vitamins, mineral elements and the like needed by the healthy culture of the Penaeus vannamei Boone are reinforced, and simultaneously the Bacillus subtilis and the taurine are added, so shelling of the shrimps is promoted, the growth performances are improved, and the disease resistance and the stress resistance of the shrimps are enhanced. The mixed feed has the advantages of reliable sources of the raw materials, stable and safe quality, difficult water pollution, and suitableness for the large-scale culture of the Penaeus vannamei Boone in the greenhouse.

The invention relates to an ecological breeding method for intercropping Siganus guttatus with Litopenaeus vannamei. The method comprises: (1) cleaning a pond before water enters the pond, after airing the pond in the sun for 18-20 days, starting to make water enter the pond, water salinity being 5-8; (2) before fry releasing, fertilizing water of a breeding pond, after three days, trying to raise Litopenaeus vannamei fries, after trial raising is successful, putting the Litopenaeus vannamei fries in the breeding pond to breed for 15 days, the density of the Litopenaeus vannamei fries being 60000-62000 per mu, and intercropping Siganus guttatus with the Litopenaeus vannamei in the breeding pond, the density of the Siganus guttatus being 100-110 per mu, and feeding Litopenaeus vannamei formula feed every day. Through the breeding method, survival rate of Siganus guttatus is high, and bred Litopenaeus vannamei individual is large, and has less disease. The method is high in average yield and obvious in breeding effect, and has good application prospect.

The invention relates to a feed additive of penaeus vannamei. The feed additive is prepared from, by weight, 10%-15% of polypeptide probiotics, 25%-30% of food-grade sodium alginate, 15%-20% of fish meal, 15%-20% of corn starch, 3%-5% of food-grade gelatin, 10%-15% of yeast hydrolysate and 5%-10% of compound vitamins, wherein a mixture of polypepti, probiotics and glucose is adopted as the polypeptide probiotics. The feed additive has the advantages that various nutrient substances such as vitamins and protein which are needed by reproduction and development of the penaeus vannamei can be met when the feed additive serves as a feed additive the penaeus vannamei; the toxicity and inflammations caused by the relatively high organic matter index in a water area to shrimp bodies can be relieved, the functions of the liver and the digestive system are improved, the wide and efficient sterilization and physical sterilization mechanism cooperates with a tissue repairing function, the comprehensive problem is solved, and great improvement is achieved from the cell tissue to the intestinal organism.

The invention belongs to the field of prawn farming and breeding, and discloses a method for the semi-closed ecological desalination cultivation of penaeus monodon. The method comprises the steps: building an ecological cultivation cement pit in a semi-closed ecological cultivation workshop, and then carrying out the ecological desalination cultivation of penaeus monodon. The method comprises the specific steps: building the ecological cultivation workshop, building an ecological desalination cultivation cement pit, clearing the bottom of the cement pit, carrying out water planting, placing seedlings, carrying out desalination management and water quality regulation and control, carrying out feeding management, and collecting young shrimps. Through the building of the semi-closed cultivation cement pit, the method achieves the desalination cultivation of the penaeus monodon, and creates a natural growth environment for the young penaeus monodon. After the desalination cultivation, the young penaeus monodon is healthy and strong and is high in adaptive capacity. The size of the young penaeus monodon can be greater than 1.5cm, and the rate of survival is improved by 25%, thereby facilitating the reduction of the cost in big water ecological cultivation of the penaeus monodon, and improving the cultivation benefits.

The invention provides a special compound feed for breeding parent prawns of penaeus monodon. The feed comprises the following components in percentage by weight: 25%-40% of fish meal, 5%-9.5% of artemia powder, 3%-6% of enzymolysis fish soluble pulp powder, 3%-9% of schizochytrium limacinum powder, 1%-3% of kelp powder, 2%-8% of hermetia illucens powder, 2%-5% of shrimp shell powder, 6%-12% of mussel powder, 2%-5% of marine red yeast dry powder, 10%-15% of flour, 1.5%-3% of lysolecithin, 0.8%-1.4% of ARA purified oil, 1.5% of DHA purified oil, 1.5% of EPA purified oil, 3.1%-3.7% of stearic acid triglyceride, 1%-2% of fish oil, 1%-5% of monocalcium phosphate, 0.9%-1.22% of vitamin premix and 1.08%-1.4% of mineral premix. According to the special compound feed, the growth performance of the parent prawns of penaeus monodon in the breeding period is remarkably improved, gonad development of the penaeus monodon is promoted, and the egg laying amount, the egg laying rate, the continuous egg laying rate, the fertilized egg hatching rate, the metamorphosis rate and the egg diameter of the parent prawns are increased.

The invention discloses a penaeus monodon test device. The penaeus monodon test device comprises a wall, a plurality of monitoring assemblies embedded in the wall and a test assembly arranged at one side of the wall, wherein the test assembly comprises a plurality of test tubes which are horizontally arranged; the heads and tails of adjacent test tubes communicate through tee joints; the pluralityof test tubes are sequentially arranged in a layered manner along a vertical direction; the plurality of test tubes can simulate sea areas with different depths; a simulation groove is arranged at the bottom part of each test tube; a seabed material is arranged in each simulation groove; the test assembly comprises supports and a plurality of probes distributed in the length direction of each support at intervals; the probes can move in the length direction of the supports; and the test assembly can monitor penaeus monodon.

The invention discloses a penaeus monodon Pm GLUT2 gene. The base sequence of the gene is as shown in SEQ ID NO: 2. The amino acid sequence of an encoded protein is shown as SEQ ID NO: 3. The invention also discloses application of the gene or the encoded protein of the gene in regulating the low-salt stress resistance of penaeus monodon. a screening method of a low-salt tolerance related SNP locus of the penaeus monodon Pm GLUT2 gene and application of the low-salt tolerance related SNP locus, screened by the method, of the penaeus monodon in penaeus monodon breeding.

The invention provides a young prawn breeding method for prawns. The young prawn breeding method includes the following steps of 1, nursery pond disinfection; 2, young prawn charging; 3, daily management. According to the young prawn breeding method for the prawns, the charging density of prawns can reach 250,000-350,000 pieces/m<3> in the cultivation process, meanwhile, the content of harmful bacteria, ammonia nitrogen and nitrous acid in the young prawn breeding process is remarkably reduced, the morbidity of the prawns is reduced, and meanwhile the survival rate of the prawns is increased. By means of the young prawn breeding method for the prawns, the charging density is high, the survival rate is high, the prawn cultivation efficiency can be improved, and the young prawn breeding cost of the prawns is reduced.

The invention belongs to the field of feeds and relates to a preparation method of a compound feed specially used for aquacultural breeding of prawns. The invention provides a preparation method of a feed especially for high-salinity breeding of penaeus vannamei. The feed can satisfy demand on nutrition for the penaeus vannamei under high salinity (higher than 3%), is good in stability in water, has strong anti-stress capability, is high in survival rate and accelerates growth, is low in feed coefficient, and is economical and practical, thereby solving the problems of slow growth, high diseases incidence and low survival rate of the penaeus vannamei under high salinity stress breeding. The feed can promote healthy development of the aquacultural breeding of prawns.

The invention discloses a penaeus monodon Na<+>/K<+>/2Cl<-> cotransporter NKCC gene. An alkali base sequence of the penaeus monodon Na<+>/K<+>/2Cl<-> cotransporter NKCC gene is as shown in SEQ ID NO:1. The invention further discloses a coding protein of the penaeus monodon Na<+>/K<+>/2Cl<-> cotransporter NKCC gene. An amino acid sequence of the coding protein is as shown in SEQ ID NO: 2. The invention further discloses application of the penaeus monodon Na<+>/K<+>/2Cl<-> cotransporter NKCC gene or the coding protein in improving the low salt tolerance of penaeus monodon and SNP loci related to low salt tolerance of the penaeus monodon. The SNP loci are the 2061<st>and/or the 2157 loci of the alkali base sequence as shown in the SEQ ID NO: 1, and an alkali base mutates into A from T. Important reference marks are provided for selecting and breeding molecules of new species of low salt tolerance of the penaeus monodon.

The invention discloses a penaeus vannamei scientific feeding method. The method includes following steps: (1), treating a breeding pond; (2), feeding penaeus vannamei: selecting feed, managing feeding, observing feed intake after feeding, and adjusting the feed at proper time; (3), adjusting breeding water. Bacteria benefitting and inhibiting principles are utilized, feed conversion rate is increased, and water quality deterioration risk is controlled; natural animal bait and compound feed are feed alternately, so that quick growth of penaeus vannamei can be realized, and bait coefficient canbe lowered, water eutrophication can be avoided, and intake, digestion and absorption of penaeus vannamei are facilitated; feeding quantity, feeding time and number of times can be correspondingly adjusted according to different growth stages, and the feed can be utilized to greatest extent; feed additive is used in the adult shrimp stage, so that necessary trace element is provided for penaeus vannamei, breeding environment is improved, immunity of penaeus vannamei is enhanced, survival rate of penaeus vannamei is increased, and economic benefit is increased.

The invention discloses a female specific primer of Penaeus chinensis and application thereof. The sequence of the female specific primer is as follows: Femal-specific-F: 5'-GTTTCACCTACGCTTCACCC-3', and Femal-specific-R is: 5'-TCCTTAATCCTGCTGCATCCA-3'. The primer is used for carrying out PCR (Polymerase Chain Reaction) amplification on genome DNA (Deoxyribose Nucleic Acid) of Penaeus orientalis to be detected, if a electrophoresis result shows that two bands of 210 bp and 392 bp appear, the Penaeus orientalis are judged to be female Penaeus chinensis, and if only the band of 392 bp appears, the Penaeus orientalis are judged to be male Penaeus chinensis. The invention also utilizes a designed contrast primer to avoid misjudgment of the identification result caused by the problem of the template, the accuracy is improved. The specific primer provided by the invention has strong specificity, and the identification method is simple, rapid and accurate, and is especially suitable for early genetic sex identification of the penaeus chinensis.

The invention discloses a compound feed special for penaeus merguiensis. The feed is prepared by adopting the following raw materials in percentage by weight: 50-70% of spray-dried hydrolyzed pig peptone, 5-10% of sunflower seed meal, 5-10% of schizochytrium powder, 10-30% of whole wheat flour, 0.5-1% of monocalcium phosphate, 0.5-1% of compound vitamin and 0.5-1% of compound mineral substance. The compound feed special for penaeus merguiensis, disclosed by the invention, is prepared according to the nutritional requirements and the digestive physiological characteristics of the penaeus merguiensis and is balanced in nutrition, simple in preparation and low in cost; and when being used for the breeding of the penaeus merguiensis, the compound feed has the following advantages that (1) the growth speed of the penaeus merguiensis is greatly increased and the feed coefficient is reduced by a large margin; (2) the raw materials are simple and easily available, and compared with existing prawn feeds, the compound feed is lower in cost; and (3) the nutrients are rich and balanced and the breeding survival rate of the penaeus merguiensis is effectively increased to more than 90%.

The invention discloses a penaeus monodon behavioral preference test system and test method. The test system comprises a test drum and a monitoring component, wherein the test drum comprises a plurality of cylinders arranged in a sleeved mode, and any cylinder can rotate; a plurality of light source pieces are arranged on the inner wall of each cylinder, and different sea substrates are arranged at the bottoms of any two adjacent cylinders; a unidirectional light-transmitting layer is arranged on the outer wall of the cylinder at the outermost side, and a plurality of unidirectional light-transmitting strips are arranged at intervals on the outer wall of any cylinder at the inner side; and the monitoring component is arranged at one side of the test drum and comprises a support and a plurality of probes arranged on the support, and the probes can perform real-time image collection on penaeus monodon.

The invention provides a molecular marker C27449 for rapidly identifying genetic sex of penaeus japonicus and application of the molecular marker C27449. The nucleotide sequence of the molecular marker C27449 is shown as SEQ ID No.1, and the nucleotide sequences of a primer pair used for detecting the molecular marker C27449 are respectively as follows: C27449F: TCATCCAAGTTCCAGCCT and C27449R: GGTCCCCCACAACATACA. The molecular marker C27449 can not be influenced by tissue specificity and environment of penaeus japonicus, use steps are simple, results are obvious, genomic DNA of the penaeus japonicus and a primer pair are subjected to PCR (polymerase chain reaction) and then electrophoresis detection, if a strip appears at 120bp, the penaeus japonicus is female penaeus japonicus, if no strip exists, the penaeus japonicus is male penaeus japonicus. The molecular marker can also promote the establishment of a penaeus japonicus all-female or high-female fry breeding technology, and has a wide application prospect.

The invention discloses a penaeus monodon female parent gonad maturation promoting small interfering RNA composition. The composition comprises small interfering RNA aiming at three hormone genes of penaeus monodon CHH, MIH and VIH for inhibiting gonad development, wherein the three small interfering RNAs are specifically CHH-siRNA, MIH-siRNA and VIH-siRNA, wherein the nucleotide sequence of a positive-sense strand of the CHH-siRNA is shown as SEQ ID NO. 1, and the nucleotide sequence of an antisense strand of the CHH-siRNA is shown as SEQ ID NO. 2; a positive-sense strand nucleotide sequenceof the MIH-siRNA is shown as SEQ ID NO. 3, and an antisense strand nucleotide sequence of the MIH-siRNA is shown as SEQ ID NO. 4; and the nucleotide sequence of the positive-sense strand of the VIH-siRNA is shown as SEQ ID NO. 5, and the nucleotide sequence of the antisense strand of the VIH-siRNA is shown as SEQ ID NO. 6. The invention also discloses a penaeus monodon female parent gonad maturation promoting test kit containing the small interfering RNA composition and a use method thereof. The small interfering RNA composition can remarkably reduce the content of hormone for inhibiting gonaddevelopment maturity of female parent shrimps, promote the generation of egg yolk protein and accelerate the gonad development process.

The invention relates to an ecological breeding method for polyculture of scylla serrata and penaeus japonicus in coastal areas of Northern Jiangsu Province. The method comprises the steps of (1) selection of a breeding pond; (2) disinfection of the breeding pond; (3) application of fertilizer and water, wherein water is fed 10 days before young scylla serrata is released, the water depth is 0.3m,20mg/L of nitrogenous fertilizer and 2mg/L of phosphatic fertilizer are applied, the pond water is dark brown or yellow green or tawny, and the transparency is 40cm; (4) releasing of young penaeus japonicus; (5) throwing of penaeus japonicus feed; (6) throwing of young scylla serrata, wherein when penaeus japonicus grows to 2-3cm, young scylla serrata is thrown according to the density of 300-400young scylla serrata per mu; (7) throwing of scylla serrata feed; (8) water quality management; (9) disease control; and (10) harvesting. By means of the breeding method, the breeding survival rate of megalopa of scylla serrata is not lower than 13%, the breeding survival rate of penaeus japonicus is not lower than 75%, the weight of bred scylla serrata individuals is 350-500g, the weight of bredpenaeus japonicus individuals is 5-10g, diseases are few, the breeding effect is remarkable, and the method has good application prospects.