32 results about "Penaeus kerathurus" patented technology

The invention relates to a penaeus monodon high temperature resistance character related SNP marker primer based on whole genome association analysis and application, and belongs to the field of molecular biology. The nucleotide sequence of the primer pair is shown in SEQ ID NO.1-2. An SNP molecular marker is located at the 27494780 site of the 27th chromosome of a penaeus monodon genome and is shown as Cgt; a mutation, and the A mutation is associated with a high temperature resistant character. The invention further provides a kit for detecting the high-temperature-resistant character of penaeus monodon, and high-temperature-resistant individuals can be screened by utilizing the primers and are used for breeding new varieties.

The invention discloses a method for increasing the survival rate of penaeus monodon in the storage and transportation process, and belongs to the field of alive-keeping transportation of aquatic products. The method comprises the steps of temporary rearing, gradient cooling dormancy treatment, oxygenation packaging and room-temperature storage. Wherein the cooling final temperature in the gradient cooling dormancy treatment step is 12 DEG C; a carrier used in oxygenation packaging is a foam box (the inner diameter is 390 mm * 270 mm * 200 mm; and the wall thickness is 20mm). By means of the method, physiological activities in the keep-alive process of the penaeus monodon can be effectively reduced, the stress response of the penaeus monodon can be reduced, the keep-alive survival rate of the penaeus monodon can be remarkably increased, meanwhile, equipment is simple, operation is easy, and certain economic benefits are achieved.

The invention discloses a penaeus monodon sperm ultralow-temperature preservation regulation and control method based on sperm motility detection, and relates to the technical field of aquatic product germplasm resources and genetic breeding, and the method comprises the following steps: collecting a penaeus monodon sperm sample, preprocessing the sample to carry out sperm motility detection, and utilizing deep learning to assist sperm motility analysis to obtain sperm motility data; collecting multiple omics data of the preprocessed sperm sample, and screening key molecular markers significantly related to sperm motility by using a knowledge graph to obtain omics characteristics; constructing a freezing injury prediction model according to the improved graph neural network, and inputting sperm motility data and omics characteristics to predict the freezing injury risk grade of the sperm sample; and dynamically adjusting a cryopreservation scheme based on the cryogenic injury risk grade of the sperm sample. According to the method, through a whole chain strategy of sperm motility detection, multi-omics analysis, artificial intelligence prediction and dynamic regulation and control, precise optimization of cryopreservation of the sperms of the penaeus monodon is realized.

The present invention discloses a relay aquaculture method for Penaeus japonicus, and relates to the field of biological aquaculture. The method employs a mixture of a protein source, a fat source, a complex vitamin, a complex inorganic salt, an attractant, and (-)-caryophyllene oxide-modified xanthan gum to prepare a bait for the relay aquaculture of Penaeus japonicus, resulting in a good survival rate, specific growth rate, and feed conversion coefficient for the Penaeus japonicus. The method also employs glucosamine-modified humic acid, which is used in the preparation of an amino acid fertilizer paste, which is then used in the relay aquaculture of Penaeus japonicus, resulting in an even better survival rate, specific growth rate, and feed conversion coefficient for the Penaeus japonicus.

The invention discloses a penaeus vannamei body size estimation method and device and a storage medium, and relates to the technical field of breeding industry image processing, and the method comprises the steps: collecting a penaeus vannamei data set image, and carrying out the marking of the penaeus vannamei data set image; constructing a penaeus vannamei body detection model, improving the penaeus vannamei body detection model, and training the improved penaeus vannamei body detection model based on the marked penaeus vannamei body data set image; and based on the trained penaeus vannamei body detection model, measuring the body size of the penaeus vannamei and estimating the length of the penaeus vannamei body. According to the method, the expression and fusion capabilities of the multi-scale features are enhanced, the positioning precision of the feature points of the prawns is improved, and a set of non-contact estimation system for the body sizes of the prawns is developed. The coordinates of the feature points of the shrimp bodies are accurately obtained, the body size indexes are calculated, and accurate estimation of the shrimps with different completeness degrees is achieved. Rapid and accurate non-contact prawn body size measurement is realized, and powerful technical support is provided for intelligent and accurate cultivation of the penaeus vannamei.

The invention discloses a probe for detecting a penaeus japonicus SNP molecular marker combination, a penaeus japonicus 31K liquid phase breeding chip and application, and belongs to the technical field of molecular marker breeding. The penaeus japonicus SNP molecular marker combination is composed of 31097 SNP molecular markers. The 31097 SNP molecular markers are as shown in the specification table 1. The invention also provides application of the penaeus japonicus liquid phase breeding chip in penaeus japonicus breeding, sample detection, genetic resource genetic diversity analysis, genetic and evolutionary analysis, genetic relationship identification, whole genome association analysis and penaeus japonicus genome selection. The penaeus japonicus 31K liquid phase breeding chip provided by the invention has the characteristics of good site representativeness, strong specificity, high polymorphism and the like, is widely applicable to detection of different varieties of penaeus japonicus materials, can establish a modern breeding system, and promotes the development of the penaeus japonicus molecular breeding industry.

The application is a deep learning-based method for breeding and multiplying Penaeus vannamei, which comprises: obtaining genomic data of the initial population; obtaining specific trait data of the screened initial population; locating a specific gene sequence set according to the specific trait data and the genomic data; the gene sequence set is a gene number set related to the specific trait; and determining the breeding shrimp for breeding according to the gene number set. The application first breeds Penaeus vannamei populations of different families, obtains the trait phenotype data and genomic data of Penaeus vannamei under different families, determines the gene sequence meeting the trait phenotype requirements according to the correlation degree between the trait phenotype data and the genomic data, that is, locates the gene sequence related to the specific trait, and then selects the next generation of Penaeus vannamei according to the gene sequence set, or directly breeds the breeding shrimp with stable traits.

The invention discloses a Metapenaeus ensis culture water regulation and control method and system based on meteorological data, and relates to the field of water quality regulation and control. Comprising the following steps: collecting metapenaeus ensis samples in different development states, determining corresponding development stages and corresponding water quality parameters, and generating a sample database; and collecting development state parameters of target metapenaeus ensis in the culture tank to be regulated and controlled, comparing the development state parameters with the sample database, and determining development stages and corresponding water quality requirements of the metapenaeus ensis. Acquiring aquaculture water quality parameters under different meteorological conditions, and establishing a neural network model; and inputting real-time meteorological data to the trained neural network model, outputting a water quality parameter prediction value, judging whether the water quality meets the water quality requirement of the target metapenaeus ensis or not, and performing adaptive regulation and control on the water quality which does not meet the requirement to ensure that the water quality parameter is maintained in a target range. According to the method, the accuracy and adaptability of water quality management of metapenaeus ensis culture are effectively improved.

The application discloses a meteorological data-based penaeus monodon cultivation water body regulation method and system, and relates to the water quality regulation field.The method comprises the following steps: collecting penaeus monodon samples in different development states, determining corresponding development stages and corresponding water quality parameters, and generating a sample database; collecting the development state parameters of target penaeus monodon in a to-be-regulated cultivation tank, comparing the development state parameters with the sample database, determining the development stage and corresponding water quality requirements of the target penaeus monodon, obtaining cultivation water quality parameters under different meteorological conditions, and establishing a neural network model; inputting real-time meteorological data into the trained neural network model, outputting water quality parameter prediction values, judging whether the water quality meets the water quality requirements of the target penaeus monodon, and adaptively regulating the water quality that does not meet the requirements, so that the water quality parameters are maintained within a target range.The method effectively improves the accuracy and adaptability of penaeus monodon cultivation water quality management.

The invention belongs to the technical field of biomolecular markers, and particularly relates to a microsatellite marker for evaluating genetic diversity of metapenaeus ensis as well as a primer pair combination and application of the microsatellite marker. By extracting genomes from different metapenaeus ensis populations, developing 10 microsatellite markers through transcriptome data and designing corresponding primer pair combinations, the microsatellite markers provided by the invention have the characteristics of stable PCR amplification result, high polymorphism and the like, can be used for researching diversity, population genetic structures, evolution, genetic relationships and the like of metapenaeus ensis, and have a wide application prospect. The method is used for evaluating genetic structures and polymorphisms of metapenaeus ensis in different areas and different populations, provides a theoretical basis for parent shrimp breeding work and resource protection of the metapenaeus ensis, and has a good application value.

The invention discloses a penaeus monodon NFAT5 gene and application thereof, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the NFAT5 gene is as shown in SEQ ID NO: 2. The invention further discloses a recombinant expression vector containing the penaeus monodon NFAT5 gene, a host cell containing the recombinant expression vector, and an application of the NFAT5 gene. The invention further discloses application of the NFAT5 gene of the penaeus monodon, the protein, the recombinant expression vector containing the gene or the host cell containing the expression vector to preparation of a gene preparation or a feed additive for improving the low-salt tolerance of the penaeus monodon.

The invention discloses a penaeus monodon DNA (deoxyribonucleic acid) fingerprint spectrum which comprises at least 209 specific SNP (single nucleotide polymorphism) sites and realizes efficient and accurate distinguishing of different culture populations, so that technical guarantee is provided for identification and traceability work of penaeus monodon germplasm, and meanwhile, an accurate molecular technology platform for germplasm resource development, utilization and protection is built. The invention further discloses a construction method of the DNA fingerprint spectrum of the penaeus monodon and application of the DNA fingerprint spectrum of the penaeus monodon in identification of germplasm resources of the penaeus monodon and assistant breeding.

This invention discloses a Penaeus monodon NFAT5 gene and its application. The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the amino acid sequence of the protein encoded by the NFAT5 gene is shown in SEQ ID NO:2. This invention also discloses a recombinant expression vector containing the Penaeus monodon NFAT5 gene, a host cell containing the recombinant expression vector, and the use of the Penaeus monodon NFAT5 gene, protein, recombinant expression vector containing the gene, or host cell containing the expression vector in the preparation of gene preparations or feed additives for improving the low salt tolerance of Penaeus monodon.

The invention belongs to the technical field of biological agents, and discloses a biological agent for preventing and treating enteritis diseases of penaeus vannamei as well as a preparation method and application of the biological agent, the biological agent comprises lactobacillus rhamnosus BY13207, and the total viable count of the biological agent is 1.5 * 10 < 9 >-3 * 10 < 9 > cfu / mL; the lactobacillus rhamnosus BY13207 is preserved in the China General Microbiological Culture Collection Center on August 11, 2025, and the preservation number of the lactobacillus rhamnosus BY13207 is CGMCC No.35559. The lactobacillus rhamnosus BY13207 added in the lactobacillus rhamnosus BY13207 has very strong antagonistic action and anti-inflammatory action, can inhibit the growth and reproduction of various pathogenic microorganisms of prawns and enhance the disease resistance of the prawns; the compound has broad-spectrum antibacterial activity, and can effectively eliminate harmful bacteria in prawn culture water and reduce the risk of disease occurrence.

The invention relates to a method for cultivating a new variety of high-temperature-resistant fast-growing penaeus monodon, which belongs to the field of aquatic breeding and comprises the following steps of: performing extreme high-temperature acute stress for 4-7 days at a juvenile penaeus monodon stage of a batch constructed family and performing high-temperature long-term cultivation stress for 45-60 days at a juvenile penaeus monodon stage; performing weighted comprehensive evaluation on growth and survival rates of different families under a high-temperature condition by combining growth parameters including average survival time of individuals of the extreme high-temperature acute stress families and average survival rates and average weights of the high-temperature long-term bred families; and screening out family individuals which grow quickly and have strong high-temperature tolerance, carrying out breed conservation and cultivation to obtain parent parents, and carrying out passage and generation breeding to obtain the new high-temperature-resistant fast-growing penaeus monodon variety.

The invention discloses a litopenaeus vannamei MAPT-LIKE gene, an SNP (Single Nucleotide Polymorphism) marker, a detection primer, a kit and application of the litopenaeus vannamei MAPT-LIKE gene. The sequence of the SNP marker is as shown in SEQ ID NO.1, the SNP site is located at the 554th site from the 5'end of the SEQ ID NO.1, and the polymorphic form is C / T type; the SNP locus can be used for breeding litopenaeus vannamei through body length or growth rate screening, and a primer designed according to the SEQ ID NO.1 sequence or a kit prepared from the primer can be used for detecting the SNP locus. According to the SNP marker Chr3541822951, parents can be selected according to the CC genotype, the body length growth speed of breeding populations can be increased, an innovative tool is provided for molecular breeding of litopenaeus vannamei, the breeding period is shortened, and industrial development and efficiency improvement are assisted.

The invention relates to the technical field of molecular immunology of prawns, in particular to a monoclonal antibody for resisting haemocyanin of Chinese prawns and application, the monoclonal antibody for resisting haemocyanin of Chinese prawns is anti-SHC-6D6 and can be expressed through a recombinant Expi293 cell of a recombinant plasmid pcDNA3.1 containing a monoclonal antibody gene for resisting haemocyanin of Chinese prawns; the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin is good in specificity, can be specifically combined to two subunits with the molecular weights of 73kDa and 75kDa of the fenneropenaeus chinensis haemocyanin, and can be used as a positive internal reference for detecting the content of the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin in animals immunized by the fenneropenaeus chinensis haemocyanin.

The application discloses a high-activity penaeus vannamei breeding pond capable of automatically adjusting breeding density and relates to the technical field of the penaeus vannamei breeding pond. The high-activity penaeus vannamei breeding pond comprises a water storage pond, a fixed frame fixedly connected to the upper portion of the water storage pond, a motor installed on the fixed frame, and a water supply pipe fixedly connected to the upper surface of the water storage pond. The high-activity penaeus vannamei breeding pond comprises an automatic pollution discharge mechanism, an automatic water supply mechanism and an automatic feeding mechanism. The automatic pollution discharge mechanism is arranged at the middle portion of the water storage pond, the automatic water supply mechanism is arranged on the upper surface of the water storage pond, and the automatic feeding mechanism is arranged on the fixed frame. The automatic pollution discharge mechanism is arranged, and automatic quantitative and timed feeding of the feed can ensure that the penaeus vannamei uniformly obtains the feed in the breeding pond, can avoid that some penaeus vannamei obtain insufficient food and nutrition, can ensure the growth and development of the whole breeding population, can fully meet the demand of the penaeus vannamei in each feeding, can reduce the problems of excessive feeding and bait waste, and can save the breeding cost.

The present application relates to a kind of penaeus vannamei ovipositor breeding ponds, including several ovipositor breeding pond bodies, the upper portion in ovipositor breeding pond body is equipped with for the reproduction of seed shrimp spawning space, the lower portion of spawning space is equipped with for the hatching of shrimp eggs ovipositor space, the bottom of ovipositor space is equipped with one side with pool wall form the ovipositor bottom plate of positionable hinged, ovipositor bottom plate includes upper portion of egg-blocking net layer, middle portion of aeration layer, lower portion of water-permeable net layer, aeration layer is equipped with multiple aeration pipes, aeration pipe is communicated with external air source, aeration pipe is distributed with aeration hole, the lower portion of ovipositor space is equipped with for the cultivation of fry breeding space, the bottom of breeding space is equipped with fry outlet on one side.The present application uses the way of pond bottom aeration to replace the existing artificial ovipositor and mechanism ovipositor mode, integrates the hatching and fry function of prawn on the ovipositor breeding pond body, with better ovipositor effect, breeding yield is improved, breeding cost is reduced, and ovipositor breeding efficiency is significantly improved, etc.

This patent discloses a cryopreservation solution and method for improving the sperm survival rate of Penaeus monodon, comprising a base solution and combined cryoprotectants. The base solution is a Penaeus monodon sperm cell culture medium containing Penaeus monodon serum; the combined cryoprotectants include permeable and non-permeable cryoprotectants. By using Penaeus monodon sperm cell culture medium instead of traditional calcium-free saline as the base solution for cryopreservation, an optimized environmental basis is established for cryopreservation, significantly improving cryoprotection efficiency. The cryopreservation method provided in this application enables Penaeus monodon sperm to achieve a survival rate of over 89% after 10 months of cryopreservation, achieving long-term stable preservation of sperm under ultra-low temperature conditions; its key biological activity indicators show no significant difference compared to before cryopreservation, successfully achieving artificial insemination. This provides key technical support for the long-term preservation of Penaeus monodon germplasm resources and lays the foundation for the construction of germplasm banks.

This invention relates to the field of aquaculture technology, specifically a feed formulation and preparation method for improving the reproductive performance of broodstock tiger prawns. The method includes: determining the specific nutritional requirements of broodstock tiger prawns during gonadal development; screening core animal and plant protein sources and determining their basic mass ratios accordingly; performing targeted biological treatment on the core raw materials to enrich gonadotropin-active small molecules; compounding these with phospholipids, attractants, vitamins, and minerals to form a functional additive combination; mixing the core raw materials and additive combination in proportion, granulating, and adding antioxidants during processing; and finally verifying the formulation effect and confirming the final compound feed through standardized aquaculture trials. The compound feed obtained based on this method can effectively replace traditional live feed, improve broodstock tiger spawning, hatching rate, and larval quality, and has advantages such as balanced nutrition, stable quality, and suitability for large-scale production, solving the industry problems of live feed being prone to pathogens, supply fluctuations, and high costs.

The present invention discloses a high-yield method for cultivating hybrid shrimp of Penaeus vannamei. The method comprises the following steps: stocking sexually mature broodstock: using common Ecuadorian-sourced Penaeus vannamei as the male parent and "Guangtai No. 1" Penaeus vannamei as the female parent, the broodstock are stocked in net cages within a pond; from the first to the 15th day after the appearance of zoeae, the entire pond is sprayed with a mixture of soybean milk and bacterial protein daily; starting from the 16th day after the appearance of zoeae, feed A daily; when the average body length of the shrimp fry reaches 0.8 to 1.0 cm, feed B daily; and when the average weight of the shrimp fry reaches 2.0 cm or more, feed C daily. The present invention integrates propagation, seedling cultivation, and farming, achieving integrated cultivation of Penaeus vannamei, saving cultivation steps, reducing costs, and achieving high yields. Furthermore, the present invention can improve the shrimp's resistance to WSSV and significantly reduce mortality.

The invention provides an MNP marker site for identifying a rapid growth type litopenaeus vannamei population, a primer group and application of the MNP marker site and the primer group, and belongs to the technical field of molecular genetics of aquatic animals. The MNP marker site, the corresponding primer group and the kit thereof can screen a litopenaeus vannamei population with the character of high growth speed, and prove that the MNP marker site can realize high-throughput analysis, avoid the generation of a large number of nonexistent error genotypes, reduce the detection cost and analysis complexity, and improve the detection efficiency. And the accuracy rate of identifying the rapid growth type litopenaeus vannamei population is up to 100%. The MNP marker site and application provided by the invention have reference significance in the related fields of molecular inheritance and genotype analysis of aquatic animals.

This invention discloses a molecular marker 03W4 for high WSSV resistance in Litopenaeus vannamei and its applications, belonging to the field of molecular marker-assisted breeding technology for aquatic animals. The nucleotide sequence of the molecular marker 03W4 is shown in SEQ ID No. 1, and the genotype TT at the 301st locus of the molecular marker 03W4 is a WSSV resistance genotype. This invention also provides primers for the molecular marker 03W4, including the amplification primers shown in SEQ ID No. 2 and SEQ ID No. 3, and the extension primer shown in SEQ ID No. 4. The molecular marker 03W4 for high WSSV resistance in Litopenaeus vannamei can be used for early selection of Litopenaeus vannamei regardless of growth stage, screening for highly WSSV-resistant individuals in breeding populations with different growth performances, rapidly selecting highly WSSV-resistant parent populations, and promoting the breeding process of new Litopenaeus vannamei varieties with high WSSV resistance.

The application relates to the field of aquaculture, in particular to a culture method of longsnout catfish (Leiocassis longirostris) in the culture of penaeus vannamei, which comprises the following steps: pond preparation, fry stocking, feed feeding, water quality management, daily management, fishing and harvesting, etc. The application establishes a multi-nutrient level culture mode of fish and shrimp symbiosis, fully utilizes the water space of the culture pond of penaeus vannamei, improves the water utilization rate of the pond, improves the production efficiency, utilizes the habits of longsnout catfish and penaeus vannamei, plays the effect of fish and shrimp ecological comprehensive disease prevention, improves the growth environment of fish, improves the feed utilization rate, reduces the total feed coefficient, reduces the wastewater discharge, and improves the energy efficiency of aquaculture. The application is simple in operation and easy to popularize.

According to the molecular marker related to the low-oxygen stress resistance of the litopenaeus vannamei, an SNP molecular marker is cloned from a PGM2 gene, and the SNP marker is a 357th basic group T or C from the 5'end of a first nucleotide sequence shown in a sequence table. According to statistics, the low dissolved oxygen tolerance of the litopenaeus vannamei with the genotype of TT at the site of the SNP marker is obviously higher than that of the litopenaeus vannamei with the genotype of TC, so that the low dissolved oxygen tolerance of the litopenaeus vannamei can be effectively determined by detecting the SNP site of the litopenaeus vannamei; the SNP marker is closely related to the low dissolved oxygen resistance character of the litopenaeus vannamei, and can be effectively used for molecular marker-assisted breeding of the litopenaeus vannamei.

The application discloses a Bacillus altitudinis JYD51 and application thereof in aquaculture, which has been preserved in the China General Microbiological Culture Collection Center on October 10, 2024, and the preservation number is CGMCC No.32164. The JYD51 strain can be fermented by using a culture medium rich in organic matter, and the fermentation product has no toxic effect on Penaeus vannamei and crabs, can inhibit the infection of hepatopancreas ciliates on the Penaeus vannamei and the harm of Paramoeba on the crabs. Meanwhile, the microbial agent prepared by the JYD51 strain and Bacillus sphaericus JYQ7 can effectively control the harm of the hepatopancreas ciliates on the Penaeus vannamei and the Paramoeba on the crabs, purify the water environment for the growth of the Penaeus vannamei and the crabs, and improve the survival rate and product quality of the Penaeus vannamei and the crabs in aquaculture.

The application discloses a BBOX1 gene molecular marker related to the Vibrio resistance trait of Penaeus vannamei, which comprises any one or more of a molecular marker A, a molecular marker B and a molecular marker C, and the molecular markers are located on a gamma-butyrobetaine dioxygenase gene sequence of the Penaeus vannamei; the molecular marker is used as a functional marker of the Vibrio resistance trait of the Penaeus vannamei, the genomic DNA of muscle tissue of the Penaeus vannamei to be detected is extracted, and then the genomic DNA is used as template DNA to perform PCR amplification and purification of a PCR amplification product, and then the product is sequenced to determine the genotypes of the molecular marker A, the molecular marker B and the molecular marker C, so that the genotypic individuals with the Vibrio resistance advantage are screened to breed the Penaeus vannamei, the obtained result is accurate and has high application value, the breeding process of the fine variety with the Vibrio resistance trait can be accelerated, and the survival rate of the shrimp culture is improved, which has important significance.

The invention belongs to the field of aquatic nutritional feed, and particularly relates to a preparation method of nereis extract and application of the nereis extract in litopenaeus vannamei feed. Comprising the following steps: (1) selecting perinereis aibuhitensis with a proper nereis development period and a proper body color; (2) disinfecting the nereis; (3) extracting effective components of nereis; and (4) applying the effective components to the litopenaeus vannamei compound feed. According to the method disclosed by the invention, the nutritional ingredients of the bred litopenaeus vannamei parents can be changed, and the content of long-chain polyunsaturated fatty acids in the bred litopenaeus vannamei parents and gonads, especially the content of DHA, is increased. The method has the advantages that synthesis of sex steroid hormones of the litopenaeus vannamei is promoted, the egg laying amount is increased by 30.3%, the multiple egg laying rate is increased by 22.1%, the average egg laying frequency is increased by 27.3%, and the nauplius metamorphosis rate is increased by 21.8%. The nereis raw material adopted by the technology has the potential of low-cost large-scale production, and has very strong operability.

The present invention relates to a feed composition suitable for feeding crustaceans comprising mannose oligosaccharides. The invention also relates to the use of mannose oligosaccharide compositions for feeding crustaceans. The invention also relates to crustaceans as a food source, in particular shrimp of the species Litopenaeus vannamei or Penaeus monodon, fed with the feed composition comprising mannose oligosaccharides.