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Gene variation site for regulating and controlling carcass indexes of Guizhou Xiang pigs and application of gene variation site

A technology of gene variation and site, applied in the field of molecular biology

Active Publication Date: 2020-10-13
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] At present, the effect and regulation mechanism of SIRT5 gene on intramuscular fat deposition and lipid metabolism in small Guizhou Xiang pigs have not been reported at home and abroad.

Method used

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  • Gene variation site for regulating and controlling carcass indexes of Guizhou Xiang pigs and application of gene variation site
  • Gene variation site for regulating and controlling carcass indexes of Guizhou Xiang pigs and application of gene variation site
  • Gene variation site for regulating and controlling carcass indexes of Guizhou Xiang pigs and application of gene variation site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1 Materials and methods

[0030] 1.1 Experimental animals and sample collection

[0031] 107 blood samples from Congjiang Xiang pig fattening pigs in Guizhou were collected with heparin sodium anticoagulant tubes for the analysis of genetic effects of SIRT5 gene variation sites. The experimental samples were all collected from Lvshengyuan Xiang pig ecological welfare farm in Guiyang City, Guizhou Province.

[0032] 1.2 Determination of carcass meat quality traits

[0033] 107 experimental pigs were weighed before slaughter, and after slaughter, the pig skin thickness, 10-11 intercostal backfat thickness and eye muscle area indicators were measured, and 300 g of longissimus dorsi muscle was collected, wrapped in tin foil, numbered, and used to measure muscle Moisture and intramuscular fat content indicators. All indicators are implemented in accordance with the agricultural industry standards of the People's Republic of China "Technical Specifications for Determination...

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Abstract

The invention discloses a gene variation site for regulating and controlling carcass indexes of Guizhou Xiang pigs and application of the gene variation site, belongs to the field of molecular biology, provides 10 SNPs sites, and provides application of the SNPs sites in regulating and controlling animal carcass performance and pig fat deposition. Correlation analysis is carried out on the detected 10 SNPs sites and 5 carcass meat quality characters of Guizhou Congjiang Xiang pigs, and the correlation between the 10 SNPs sites and test group characters is defined; according to the invention, amolecular basis can be provided for breeding pig carcass traits in the future, and a foundation is laid for genetic improvement of pig fat traits by using the gene in the future.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a gene variation site for regulating the carcass index of Guizhou fragrant pig and its application. Background technique [0002] In recent years, due to the excessive pursuit of high lean meat percentage in pig breeding work, the quality of pork has seriously declined. With the continuous improvement of living standards, people have higher and higher requirements for meat quality characteristics such as pork color, tenderness, and flavor. In the past ten years, pigs have been selected mainly for pork production requirements such as increased fat deposition and carcass leanness, but often resulted in reduced fat storage and deposition. This type of selection also results in a gradual reduction in total lipid content in the muscle, especially in breeds where one tends to select for lean mass deposits. For example, large white selection has significantly reduced marbling levels, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 张雄史开志王婧张静杜春林赵春萍张林鑫
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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