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Novel short nucleotide tandem repeat sequence sites and use thereof

A short tandem repeat and nucleotide sequence technology, applied in the field of short nucleotide tandem repeat sequence sites, can solve problems such as poor compatibility

Active Publication Date: 2014-09-17
GENESKY DIAGNOSTICS SUZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, in order to overcome defects such as poor compatibility, presence of CNV or location in highly repetitive sequence regions, a new type of short tandem repeat sequence with high discrimination is still needed in this field

Method used

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  • Novel short nucleotide tandem repeat sequence sites and use thereof
  • Novel short nucleotide tandem repeat sequence sites and use thereof
  • Novel short nucleotide tandem repeat sequence sites and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0131] Combining the new STR locus G5S0003 of the present invention with 10 commonly used loci (VWA, D16S539, D5S818, D7S820, D13S317, D8S1179, D18S51, TH01, D2S1338, AMELO), multiplex fluorescent PCR technology was used to check the samples, and the four used The samples were three samples from a related family and one unrelated control sample. The primer sequences of G5S0003 and 10 commonly used loci are shown in Table 2.

[0132] Table 2 Primer Sequence

[0133]

[0134] Note: PET, VIC, NED, FAM four fluorescent dyes ((Applied Biosystems, USA company).

[0135] The specific experimental steps are as follows:

[0136] 1) DNA sample preparation

[0137] Collect blood from 4 selected sample individuals; extract DNA samples through DNA extraction kit and take 1 μl each, perform quality inspection and concentration estimation on the samples by 1% agarose electrophoresis, and then dilute the samples to the working concentration according to the estimated concentration 5-10...

Embodiment 2

[0150] Randomly select 20 groups of related and unrelated samples, and use the method of Example 1 for detection, the difference is that only the new STR locus G5S0003 of the present invention is used.

[0151] After testing, after determining the paternal sample, determine the offspring samples that are related to the male parent in the remaining 19 groups of samples. The new STR locus G5S0003 of the present invention can accurately exclude 11 groups of unrelated samples. A higher degree of discrimination was obtained.

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Abstract

The invention discloses novel short nucleotide tandem repeat sequence sites and a use thereof. The sequence of the short tandem repeat sequence gene-locus G5S0003 is shown in the formula of SEQ ID NO: 1. The short tandem repeated sequence gene-locus G5S0003 is used for preparation of (a) a reagent or a kit for genetic relationship analysis, (b) a reagent or a kit for individual identification, (c) a reagent or a kit for paternity testing or blood relationship analysis, (d) a reagent or a kit for detecting if amniotic fluid extract is polluted by mother blood, and / or (e) a kit for detecting if leucocytes in an acceptor subjected to bone marrow transplantation are replaced by donor cells. The short tandem repeat sequence gene-locus G5S0003 has high discrimination and can realize effective analysis of a genetic relationship.

Description

technical field [0001] The invention relates to a short nucleotide tandem repeat sequence site and its application. Background technique [0002] Short Tandem Repeat (Short Tandem Repeat.STR), also known as microsatellite DNA (microsatellite DNA), is a type of DNA tandem repeat sequence with a repeat unit of 2-6bp, a repeat number of 10-60, and a fragment length of less than 400bp; The reason is slippage during DNA replication, or base mismatch between the slipping strand and the complementary strand during replication and repair, resulting in the deletion or insertion of one or several repeat units. STR has a wide distribution in the genome, many alleles, high heterozygosity, and stable typing results. STR inheritance conforms to Mendelian inheritance law (Koreth, J., et al.1996.Microsatellites and PCR ge-nomic analysis.J .Pathol.178:239-248.) and other characteristics, and when multiple STR loci are jointly detected, the individual recognition ability and non-parent exclu...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 姜正文马瑞晓张希
Owner GENESKY DIAGNOSTICS SUZHOU
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