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Common intertidal shellfish hybridization method

A hybridization method and breeding technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as poor germplasm resistance to stress and slow growth rate, improve survival traits, prevent progeny decline, and enhance heterozygosity. Effect

Active Publication Date: 2011-07-20
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention aims to solve the problems of poor germplasm stress resistance and slow growth speed of current tidal flat shellfish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The first step, collection of Philippine clam parents of Dalian population and Putian population: In September 2003, 2-year-old healthy and intact Philippine clam parents of Dalian population and Putian population were selected. Among them, the Putian population in China grows fast, has thin shells, and has strong heat resistance; the Dalian population has moderate individual size, thick shells, and strong cold resistance.

[0018] The second step, the ecological maturation promotion of the parents of Philippine clams of two geographical groups: the collected parents were placed in the shrimp pond for ecological maturation promotion. After 12 months of bottom sowing cultivation, in September 2004, the gonads matured synchronously .

[0019] The third step is the simple hybridization between the two populations: use an electric drill with a diameter of 0.6mm to identify the gender of the parents. After the identification, the parents can be stimulated by drying in the sh...

Embodiment 2

[0025] The first step, collection of Philippine clam parents from China's Guangxi population and Japan's Tokyo population: In September 2004, 2-year-old healthy and undamaged Philippine clam parents from China's Guangxi population and Japan's Tokyo population were selected. Among them, the Guangxi population in China has large individuals, thin shells, and strong heat resistance; the Tokyo population in Japan has moderate individual size, thick shells, and strong cold resistance.

[0026] The second step, the ecological maturation promotion of the parents of Philippine clams of two geographical groups: the collected parents were placed in the shrimp pond for ecological maturation promotion. After 12 months of bottom sowing cultivation, in September 2005, the gonads matured synchronously .

[0027] The third step, simple breeding and hybridization between two populations: Use an electric drill with a diameter of 0.6mm to identify the gender of the parents. After identification,...

Embodiment 3

[0033] The first step is to collect the Philippine clam parents of the Chinese Putian population and the Japanese Tokyo population: in September 2005, select the 2-year-old healthy and undamaged Philippine clam parents of the Chinese Putian population and the Japanese Tokyo population. Among them, the Putian population in China grows fast, has thin shells, and has strong heat resistance; the Tokyo population in Japan has moderate individual size, thick shells, and strong cold resistance.

[0034] The second step, the ecological maturation promotion of the parents of Philippine clams of two geographical groups: the collected parents were placed in the shrimp pond for ecological maturation promotion. After 12 months of bottom sowing cultivation, in September 2006, the gonads matured synchronously .

[0035] Step 3: Simple breeding and hybridization between two populations: Use an electric drill with a diameter of 0.6mm to identify the gender of the parents. After identification,...

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Abstract

The invention discloses an improved crossing method of a common mudflat shellfish, comprising the steps: secondary instar parents of the mudflat shellfish from different geographic populations are collected; the sexual gland is matured simultaneously by eco-maturing; simple improved crossing between two geographic populations is carried out; a first filial generation of simple improved crossing is bred up by a three-stage method; the first filial generation of cross-breeding is carried out the improved crossing with an introduced population; the first filial generation of cross-breeding is carried out the progressive improved crossing with a native population so as to obtain a filial generation of improved crossing; on the basis of that, the steps are repeated, the filial generation of improved crossing is carried out back crossing with the parent population so as to lead the filial generation of cross-breeding to maintain the heterosis thereof. The operation of the method is simple and convenient, and has high practicability, effectively enhances the resistance capability of the mudflat shellfish against the drastic changes of environmental factors, namely, temperature, salinity and the like, causes the improvement of the growth and survivability thereof and leads the maintenance of heterosis, and lays the foundation for the development of the fine variety of mudflat shellfish with high yield and stress resistance.

Description

technical field [0001] The invention relates to a genetic breeding technology of marine shellfish, specifically a method for breeding and hybridizing two common tidal flat shellfish with large genetic difference geographical groups. Background technique [0002] Hybrid breeding is the main way to breed new varieties. By selecting varieties, strains, populations and individuals with excellent traits for hybridization, breeding hybrid populations that meet the breeding requirements has important guiding and practical significance for production. Hybridization can recombine the genes of the parents to form various types, providing abundant materials for selection; gene recombination can combine the excellent genes of the parents that control different traits, or combine the different minor genes that control the same trait in the parents Cumulatively, a type that surpasses the parent in each of these traits is produced. [0003] Breeding hybrids include simple breeding hybrid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 闫喜武张跃环霍忠明杨凤张国范王金海刘洋
Owner DALIAN FISHERIES UNIVERSITY
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