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Novel short nucleotide tandem repeat sequence sites and use thereof

A short tandem repeat and nucleotide sequence technology, applied in the field of short nucleotide tandem repeat sequence sites, can solve problems such as poor compatibility

Active Publication Date: 2014-09-17
GENESKY DIAGNOSTICS SUZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, in order to overcome defects such as poor compatibility, presence of CNV or location in highly repetitive sequence regions, a new type of short tandem repeat sequence with high discrimination is still needed in this field

Method used

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  • Novel short nucleotide tandem repeat sequence sites and use thereof
  • Novel short nucleotide tandem repeat sequence sites and use thereof
  • Novel short nucleotide tandem repeat sequence sites and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] The new STR locus G8S0001 of the present invention was combined with 10 commonly used loci (VWA, D16S539, D5S818, D7S820, D13S317, D8S1179, D18S51, TH01, D2S1338, AMELO), and the samples were examined by multiplex fluorescence PCR technology, and 4 were used. The samples were a family of three with kinship, and a control sample without kinship. The primer sequences of G8S0001 and 10 commonly used loci are shown in Table 2.

[0131] Table 2 Primer sequences

[0132]

[0133] Note: PET, VIC, NED, FAM four fluorescent dyes ((Applied Biosystems, USA company).

[0134] The specific experimental steps are as follows:

[0135] 1) DNA sample preparation

[0136] Blood was collected from 4 selected sample individuals; DNA samples were extracted by DNA extraction kits and 1 μl of each was taken, and the samples were subjected to quality inspection and concentration estimation by 1% agarose electrophoresis, and then the samples were diluted to the working concentration accor...

Embodiment 2

[0148] 20 groups of samples with and without kinship were randomly selected and detected by the method of Example 1, except that only the new STR locus G8S0001 of the present invention was used.

[0149] After testing, after the paternal sample is determined, the offspring samples that are related to the paternal parent in the remaining 19 groups of samples are determined. The new STR locus G8S0001 of the present invention can accurately exclude 14 groups of unrelated samples. a higher degree of distinction.

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Abstract

The invention discloses a novel short nucleotide tandem repeat sequence sites and a use thereof. The sequence of the short tandem repeat sequence gene-locus G8S0001 is shown in the formula of SEQ ID NO: 1. The short tandem repeat sequence gene-locus G8S0001 is used for preparation of (a) a reagent or a kit for genetic relationship analysis, (b) a reagent or a kit for individual identification, (c) a reagent or a kit for paternity testing or blood relationship analysis, (d) a reagent or a kit for detecting if amniotic fluid extract is polluted by mother blood, and / or (e) a kit for detecting if leucocytes in an acceptor subjected to bone marrow transplantation are replaced by donor cells. The short tandem repeat sequence gene-locus G8S0001 has high discrimination and can realize effective analysis of a genetic relationship.

Description

technical field [0001] The present invention relates to a short nucleotide tandem repeat sequence site and its application. Background technique [0002] Short tandem repeat (Short Tandem Repeat.STR), also known as microsatellite DNA (microsatellite DNA), is a type of DNA tandem repeat with a repeat unit of 2-6 bp, a repeat number of 10-60, and a fragment length of less than 400 bp; its production The reason is the slippage during DNA replication, or the base mismatch between the slippery strand and the complementary strand during replication and repair, resulting in the deletion or insertion of one or several repeat units. STR has a wide distribution in the genome, many alleles, high heterozygosity, and stable typing results. .Pathol.178:239-248.) and other characteristics, and when multiple STR loci are jointly detected, the individual identification and non-paternal exclusion rate are high. Therefore, STR, due to its genetic polymorphism and other characteristics, has b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68C12N15/10
Inventor 姜正文马瑞晓张希
Owner GENESKY DIAGNOSTICS SUZHOU
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