Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying purity of Jingnongke 728 corn hybrids based on SNP markers

A technology of hybrid purity and corn, applied in the field of molecular biology of crops, can solve the problems of being easily affected by the environment or developmental stage, difficult for high-throughput detection methods, and heavy workload.

Active Publication Date: 2020-01-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported methods have problems such as long identification cycle, heavy workload, easy to be affected by environment or developmental stage, and difficult to form high-throughput detection methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying purity of Jingnongke 728 corn hybrids based on SNP markers
  • Method for identifying purity of Jingnongke 728 corn hybrids based on SNP markers
  • Method for identifying purity of Jingnongke 728 corn hybrids based on SNP markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Determination of SNP molecular markers and primers for identifying the purity of Jingnongke 728 corn hybrids

[0038] (1) Basic loci: use the 384 SNP loci published in "Maize DNA Fingerprint Library and Variety Molecular Identification SNP Core Locus Combination-maizeSNP384" (disclosed in Chinese Patent 201410756086.0) as the starting locus set, and established SNP-DNA fingerprint data of 335 nationally approved maize hybrids.

[0039] (2) Test of SNP sites and evaluation of biological characteristics: 192 corn hybrids were selected, and DNA was extracted by rapid method extraction, CTAB, kits, and various methods. Based on the KASP technology system, DNA verification sites extracted by different methods were used. The typing effect, repeatability, and stability of the points are obtained to obtain a set of candidate points.

[0040] (3) Evaluation of SNP polymorphism: Using 200 different types of corn inbred lines and 335 corn hybrid data, evaluate the polym...

Embodiment 2

[0049] Embodiment 2 Utilizes the method for identifying the purity of Jingnongke 728 corn hybrids using the SNP molecular marker provided by the present invention

[0050] Using the primer combination designed in Example 1 for 4 SNP sites, the scheme for identifying the seed purity of Jingnongke 728 is as follows: synthesize primers according to the KASP technical requirements, and the primers are ordinary primers without fluorescent groups; purchase KASP technical support The PCR amplification system MasterMix; configure the reaction system to add DNA, primers and MasterMix; run the amplification reaction program; scan the fluorescence signal in situ; perform data analysis to obtain genotype data; Based on the genotype data above, it is judged whether the individual plant is a normal plant, a self-bred seedling, or a heteromorphic plant, and finally its purity value is comprehensively judged. The specific operation is as follows:

[0051] (1) Preparation of samples to be tes...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a method for identifying purity of Jingnongke 728 corn hybrids based on SNP markers. Based on 384 SNP sites, a high-throughput KASP technology platform is used, 500 or more samples are used for comprehensive evaluation of experimental effects, biological characteristics and polymorphic parameters, 4 of the SNP markers with high quality, high stability, high polymorphism and high heterozygosity are finally determined, 3 primers for each SNP marker and a total of 12 primers are designed and amplified. Nucleotide sequences are shown in SEQ ID NO.1-12, respectively.The 4 pairs of the primer combinations can be used to identify seed purity of Jingnongke 728 and can identify selfing, backcrossing, and heteromorphic plants. Types and methods of the purity identification markers for the corn hybrids are expanded to provide a strong technical guarantee for promoted planting of the Jingnongke 728.

Description

technical field [0001] The invention belongs to the technical field of crop molecular biology, and in particular relates to a method for identifying the purity of Jingnongke 728 corn hybrid based on SNP markers. Background technique [0002] Seeds are the basic means of production in agriculture, and the yield-increasing potential of fine varieties is easily affected by the purity of seeds. Purity identification is one of the indicators for judging whether the quality of commercialized seeds is qualified. It is also an important means to prevent counterfeit and inferior seeds from entering the market, and it is also an important way for seed companies to control product quality. Establishing a fast, efficient, low-cost, and high-throughput seed purity identification method is very conducive to improving seed quality monitoring, promoting standardized production and management, and protecting the vital interests of seed companies and farmers. [0003] The traditional field i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 田红丽王蕊杨扬易红梅王元东
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com