Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New short nucleotide tandem repeat sequence locus and application thereof

A short tandem repeat and nucleotide sequence technology, applied in the field of short nucleotide tandem repeat sequence sites, can solve problems such as poor compatibility

Active Publication Date: 2014-08-06
GENESKY DIAGNOSTICS SUZHOU
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, in order to overcome defects such as poor compatibility, presence of CNV or location in highly repetitive sequence regions, a new type of short tandem repeat sequence with high discrimination is still needed in this field

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New short nucleotide tandem repeat sequence locus and application thereof
  • New short nucleotide tandem repeat sequence locus and application thereof
  • New short nucleotide tandem repeat sequence locus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0129] Combining the new STR locus G2S0002 of the present invention with 10 commonly used loci (VWA, D16S539, D5S818, D7S820, D13S317, D8S1179, D18S51, TH01, D2S1338, AMELO), multiplex fluorescent PCR technology was used to check the samples, and the four used The samples were three samples from a related family and one unrelated control sample. The primer sequences of G2S0002 and 10 commonly used loci are shown in Table 2.

[0130] Table 2 Primer Sequence

[0131]

[0132] Note: PET, VIC, NED, FAM four fluorescent dyes ((Applied Biosystems, USA company).

[0133] The specific experimental steps are as follows:

[0134] 1) DNA sample preparation

[0135] Collect blood from 4 selected sample individuals; extract DNA samples through DNA extraction kit and take 1 μl each, perform quality inspection and concentration estimation on the samples by 1% agarose electrophoresis, and then dilute the samples to the working concentration according to the estimated concentration 5-10...

Embodiment 2

[0147] Randomly select 20 groups of related and unrelated samples, and use the method of Example 1 for detection, the difference is that only the new STR locus G2S0002 of the present invention is used.

[0148] After testing, after determining the paternal sample, determine the offspring samples that are related to the male parent in the remaining 19 groups of samples. The new STR locus G2S0002 of the present invention can accurately exclude 12 groups of unrelated samples. A higher degree of discrimination was obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a new short nucleotide tandem repeat sequence locus and application thereof. The invention provides the short tandem repeat locus G2S0002, the sequence of which is shown as SEQ ID NO.:1, and the short tandem repeat locus G2S0002 can be used for preparation of: (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity test or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and / or (e) kits for detecting whether white blood cells in a bone marrow transplantation receptor is replaced by donor cells. The short tandem repeat locus G2S0002 has a high degree of distinction, and can effectively analyze the genetic relationship.

Description

technical field [0001] The invention relates to a short nucleotide tandem repeat sequence site and its application. Background technique [0002] Short Tandem Repeat (Short Tandem Repeat.STR), also known as microsatellite DNA (microsatellite DNA), is a type of DNA tandem repeat sequence with a repeat unit of 2-6bp, a repeat number of 10-60, and a fragment length of less than 400bp; The reason is slippage during DNA replication, or base mismatch between the slipping strand and the complementary strand during replication and repair, resulting in the deletion or insertion of one or several repeat units. STR has a wide distribution in the genome, multiple alleles, high heterozygosity, and stable typing results. STR inheritance conforms to Mendelian inheritance laws (Koreth, J., et al. 1996. Microsatellites and PCR ge-nomic analysis. J. Pathol.178:239-248.) and other characteristics, and when multiple STR loci are jointly detected, the individual recognition ability and non-pare...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/11C12Q1/68C12N15/10
Inventor 姜正文马瑞晓张希
Owner GENESKY DIAGNOSTICS SUZHOU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com