Culture method of halotolerant bacteria capable of removing nitrate in high-salinity solution and application thereof

Abstract

The invention discloses a culture method of halotolerant bacteria capable of removing nitrate in a high-salinity solution, which comprises the following steps: 1) adding a sodium chloride solution into a stirring tank filled with a waste liquid, stirring and carrying out trace aeration, and controlling the concentration of sodium chloride to be 25-35g / L; and 2) continuously adding nitrified sludgeinto the stirring tank, adding glucose to control the COD content to be 1800-2200mg / L, culturing for 2-4 days, adding potassium nitrate, controlling the NO3<-> concentration to be 400-600mg / L, reacting for 60-84 hours, emptying a water sample, and retaining activated sludge, thereby obtaining the halotolerant bacteria. According to the method, the high-salt-tolerance microorganisms can be cultured, and the nitrate removal effect is good. According to the culture method of the halotolerant bacteria, the high-salt-tolerance microorganisms can be cultured, the culture time is short, the cost islow, the occupied area is small, the living conditions of the microorganisms are wide, temperature and pH changes (except for rapid changes) can be borne, and the effect of removing nitrate in the high-salinity solution is good.

Description

technical field [0001] The invention relates to a cultivation method and application of a salt-tolerant bacteria capable of removing nitrate in a high-salt solution, and belongs to the technical field of wastewater treatment. Background technique [0002] There are two conventional processes for removing nitrate in high-salt concentrated liquid, namely denitrification process and anaerobic ammonium oxidation process. [0003] The denitrification process has the following disadvantages: 1. It is difficult to cultivate high-salt-resistant microorganisms, and the general cultivation period is longer than 3 months. 2. If the strains are introduced, the salinity of the water to be treated is different from that of the cultured water, the strains will lose their activity, die in large areas, and cannot be used. 3. Excessively high salinity, microorganisms cannot be cultured and survived in large numbers. 4. The general reaction requires COD: nitrate to be 1:3, and the dosage of ...

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Problems solved by technology

[0003] The denitrification process has the following disadvantages: 1. It is difficult to cultivate high-salt-resistant microorganisms, and the general cultivation period is longer than 3 months

2. If the strains are introduced, the salinity of the water to be treated is different from that of the cultured water, the strains will lose their activity, and a large area will die, making it unusable

3. Excessively high salinity, microorganisms cannot be cultivated and survived in large numbers

[0004] Disadvantages of the anaerobic ammonium oxidation process: 1. Microorganisms can hardly be cultivated, and the domestication period is generally longer than 2 years

2. The cost of introducing strains is high

3. The survival conditions are harsh, and fluctuations in temperature and pH will lead to the death of microorganisms in large areas

[0005] Therefore, in the existing nitrate removal process, there are problems such as difficulty in cultivating salt-tolerant microorganisms, high-salt (50g / L NaCl and above) concentrated liquid microorganisms can hardly survive, long cultivation period of salt-tolerant microorganisms, and large equipment volume.

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