Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Screening method of salt-tolerant strain capable of rapidly degrading organic macromolecules

A rapid degradation technology for organic macromolecules, applied in the field of microorganisms, can solve the problems of screening result errors, ignoring the screening of highly salt-tolerant strains, lack of screening of salt-tolerant strains, etc., and achieve the effect of improving screening efficiency

Pending Publication Date: 2020-08-25
ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the strains that single-degrade organic macromolecules are selected for screening, while the screening of highly salt-tolerant strains is ignored, and the method for screening salt-tolerant strains that can quickly degrade various organic macromolecules is lacking; at the same time, due to different microbial strains The growth ability is different, and the screening method of the prior art does not take into account the influence of this aspect, and there are obvious errors in the screening results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Screening method of salt-tolerant strain capable of rapidly degrading organic macromolecules
  • Screening method of salt-tolerant strain capable of rapidly degrading organic macromolecules
  • Screening method of salt-tolerant strain capable of rapidly degrading organic macromolecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Screening of salt-tolerant bacteria using food waste heaps as samples

[0031] S1. Collection of samples from the source of salt-tolerant bacteria: Select the sample for screening salt-tolerant bacteria during the composting process of kitchen waste, randomly select 5 points for the S type of each sample, use the quartering method, mix well, and put them in ziplock bags. The fresh compost samples should be removed from the debris with larger particle size and stored in a -4 ℃ incubator.

[0032] S2. Prepare LB selective medium containing NaCl: weigh 10g of reagent tryptone, 5g of yeast extract, 10g of NaCl, 1000ml of deionized water, 20g of agar powder, or directly use 40g of prepared LB medium reagent and add 1000ml of deionized The water is stirred until the solute is dissolved. Add additional NaCl 10g, 30g, 50g, 70g of volume fraction salinity (that is, corresponding to salinity 1%, 3%, 5%, 7%), adjust the pH to 7.0 with 5mol / L NaOH, and sterilize it by steam under ...

Embodiment 2

[0046] Screening of salt-tolerant bacteria using food waste compost products as samples

[0047] The screening method of this embodiment is the same as that of Example 1, the difference is that the sample type in step S1 is changed to a kitchen waste compost product, and 4 strains of salt-tolerant bacteria that can rapidly degrade organic macromolecules are obtained in this embodiment. The specific information The following table:

[0048]

Embodiment 3

[0050] Screening of salt-tolerant bacteria using food waste compost leachate soil as a sample

[0051] The screening method of this embodiment is the same as that of Example 1, the difference is that the sample type in step S1 is changed to kitchen waste compost leachate soil, and 14 salt-tolerant bacteria strains that can rapidly degrade organic macromolecules are obtained. The information is as follows:

[0052]

[0053]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a screening method of a salt-tolerant strain capable of rapidly degrading organic macromolecules. The screening method comprises the following steps of collecting a salt-tolerant bacterium source sample; preparing an LB selective culture medium containing NaCl; treating the sample; separating and purifying a salt-tolerant strain; inoculating and culturing halotolerant bacteria; preparing an organic macromolecular hydrolysis culture medium; and carrying out hydrolysis function verification. According to the screening method, errors caused by plate hydrolysis results dueto different strain inoculum sizes are eliminated, all strains to be tested are adjusted to the same absorbance for experiments, the capacity of the strains to generate hydrolase under the same culture condition of different halotolerant bacteria is evaluated through a 'time-UP value' data relation, the screening efficiency is improved, the salt-tolerant strains capable of rapidly degrading organic macromolecules can be conveniently, rapidly and accurately screened, so that salt-tolerant strain resources are provided for subsequent research and development of kitchen waste composting inoculants.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a screening method for salt-tolerant strains that rapidly degrade organic macromolecules. Background technique [0002] The core of microbial fermentation technology treatment is microbial strains. Different strains have different environmental adaptation characteristics and enzyme activities. The selection of environmental microorganisms for different purposes is the focus of microbial fermentation treatment. The selection of microbial strains based on organic matter, high fat and other characteristics is the key point to use microbial mixed fermentation technology to treat kitchen waste. It is necessary to screen microorganisms with high salt tolerance and degradable organic macromolecules for the treatment of kitchen waste, realize the resource utilization of kitchen waste, and help the construction of an eco-friendly society. [0003] In the process of decomposing org...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/02C12Q1/04G01N21/31
CPCC12N1/02C12Q1/04G01N21/31
Inventor 丁国春李季陈彦廷孟雅
Owner ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com