Method suitable for rapid enrichment culture of halophilic and halotolerant bacteria

Abstract

The invention provides a method suitable for the rapid enrichment culture of halophilic and halotolerant bacteria. The method comprises the following steps: 1, carrying out 10 times dilution on filtered, decolored and deoiled wastewater by using distilled water; 2, preparing a culturing medium composed of 3-6g of hydrolyzed casein, 5-10g of a beef extract, 5-10g of tryptone, 0.5-1g of KHPO4, 0.5-1g of K2HPO4, 2-5g of KCl, 0.05g of MgSO4.7H2O, 0.05g of CaCl2 and 0.05g of FeSO4.7H2O; 3, dissolving components composing the culturing medium in 1L of the diluted wastewater solution obtained in step 1, adding 10g of NaCl, and adjusting the pH value to 7.0-8.0 by using NaOH; and 4, placing a solution obtained in step 3 on a shaking bed, culturing at 125rpm at a temperature of 30DEG C, adding 11g of NaCl and 100ml of the culturing medium obtained in step 2 every 14h, and culturing for 70h to obtain a halophilic and halotolerant bacterium mixture.

Description

technical field [0001] The invention relates to the treatment of high-salt wastewater. The high-salt wastewater produced by organic chemical industry, coal chemical industry, textile printing and dyeing, petroleum exploitation, etc. is subjected to rapid enrichment and culture to obtain halophilic and halophilic bacteria, and the effect is extremely remarkable. Background technique [0002] High-salt wastewater refers to wastewater containing a certain concentration of organic matter with a salt mass fraction ≥ 1%, which contains a large amount of inorganic salts, such as Cl - , S0 4 2- , Na, Ca 2+ Plasma, the current methods of treating high-salt wastewater mainly include physical methods, physical-chemical methods and biological methods, among which the treatment costs of physical and physical-chemical methods are relatively high. Therefore, biological methods have become the first choice for most high-salt wastewater treatment processes. The preferred method, however, ...

AI Technical Summary

Problems solved by technology

[0002] High-salt wastewater refers to wastewater containing a certain concentration of organic matter with a salt mass fraction ≥ 1%, which contains a large amount of inorganic salts, such as Cl - , S0 4 2- , Na, Ca 2+ Plasma, the current methods of treating high-salt wastewater mainly include physical methods, physical-chemical methods and biological methods, among which the treatment costs of physical and physical-chemical methods are relatively high. Therefore, biological methods have become the first choice for most high-salt wastewater treatment processes. The preferred method, however, the inorganic salts in high-salt industrial wastewater have a strong inhibitory effect on general microorganisms, different bioreactors have different effects on high-salt wastewater, and most microorganisms in ordinary biological methods can only tolerate salt For waste water below 3%, when the salt content is greater than 5%, its treatment effect will be greatly reduced. Therefore, the use of ordinary microorganisms to treat high-salt waste water needs to be diluted, which will cause waste of water resources. A new process of desalination, dilution and direct biological treatment, that is, the process of inoculating and improving aerobic activated sludge with halophilic bacteria or halophilic bacteria, after screening and cultivating, a stable treatment effect is produced