Preparation method of salt-tolerant biological agent and adding method thereof
A technology of biological inoculants and salt-tolerant bacteria, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of reduced BOD5 removal rate, reduced removal rate, and poor effluent quality.
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[0030] The preparation method of a salt-tolerant biological bacterial agent shown in the present invention, the salt-tolerant biological bacterial agent comprises a salt-tolerant high-efficiency bacterial agent and a salt-tolerant high-efficiency bacterial filler, and the salt-tolerant high-efficiency bacterial agent is prepared by the salt-tolerant bacteria O1 and the resistant bacteria. The salt-tolerant and high-efficiency bacterial filler is prepared by mixing and compounding the salt-resistant bacteria Y5. The salt-resistant and high-efficiency bacterial filler is made by immobilizing the salt-resistant high-efficiency bacterial agent and loading it on the polyurethane filler. , The salt-tolerant biological agent can treat high-salt wastewater through microbial decomposition. The salt-tolerant biological agent includes the salt-tolerant high-efficiency bacterial agent and the salt-tolerant high-efficiency bacterial filler. The salt-tolerant bacteria O1 and the salt-tolerant...
Embodiment 1
[0045] Example 1. The experiment records the effect of compound high-efficiency salt-tolerant bacteria with different ratios on the removal effect of organic pollutants in sewage, inoculated into domestic sewage with a salinity of 3%, and measured the removal effect of organic pollutants, so as to explore O1 and The optimal configuration ratio of two high-efficiency salt-tolerant bacteria in Y5;
[0046] The O1 and Y5 salt-tolerant bacteria suspensions were prepared into 107CFU / ML bacterial suspensions according to the ratios of 0 / 100, 25 / 75, 50 / 50, 75 / 25, and 100 / 0, respectively, and inoculated to a salinity of 3%. In the domestic sewage, shake culture at 25 °C and 150 r / min. The sampling period was 24 hours, and the samples were continuously sampled for 10 days. The samples were centrifuged and passed through a 0.5 micron membrane to determine the TOC value. Depend on figure 1 It can be seen from the experimental results that the maximum degradation rate of organic matter...
Embodiment 2
[0047] Embodiment 2. Use immobilization means to prepare salt-tolerant bacteria filler, prepare a salt-tolerant and efficient bacteria agent and salt-tolerant and efficient bacteria filler, wherein the salt-tolerant and efficient compound bacteria filler prepared by immobilization method can prolong the degradation of TOC by salt-tolerant and efficient bacteria The timeliness is divided into the following two aspects:
[0048] The O1 and Y5 salt-tolerant bacteria that have been screened in the laboratory were inoculated into LB liquid medium with salinity of 5% and 7%, respectively, and shaken at 25 ° C and 150 r / min, and the OD600 was measured every two hours. , when the OD600 values of strains Y1 and Q5 reached 3.10 and 1.39, respectively, the bacterial concentration in the LB liquid medium could be measured to reach 107 CFU / ml by the line drawing method. The LB liquid medium containing the two strains of bacteria was mixed at 1:1, the supernatant was removed by centrifuga...
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