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Preparation method of salt-tolerant biological agent and adding method thereof

A technology of biological inoculants and salt-tolerant bacteria, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of reduced BOD5 removal rate, reduced removal rate, and poor effluent quality.

Inactive Publication Date: 2019-12-13
芜湖青弋环保科技有限责任公司
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Problems solved by technology

Dale et al. studied the use of biofilters to treat petrochemical wastewater containing high concentrations of salt and organic matter. The biofilm activity was inhibited by high concentrations of salts, resulting in a decrease in the removal rate of BOD5; Dincer et al. used a biological turntable system to treat wastewater with different salinities. The law of the COD removal rate changing with the salt concentration was studied, and the results showed that the COD removal rate decreased with the increase of the salt concentration, and when the salt concentration was 5%, the COD removal rate could be reduced to 65%; Cui Youwei et al. A feasibility study on the biological treatment of seawater flushing sewage was carried out by using the SBR process. The experiment simulated the salinity of seawater by adding NaCl. The research concluded that the NaCl mass concentration of 0.5% is beneficial to the growth of microorganisms, and the biological treatment NaCl mass concentration 2.5%, the treatment effect will deteriorate, and the effluent quality will not even meet the secondary discharge standard
In addition, during the start-up period of the biological treatment process, the domestication of the activated sludge has not been completed. Due to the high concentration of the activated sludge, the metabolic function of the microorganisms is destroyed, and the number of protozoa in the sludge is drastically reduced, resulting in a slow domestication of the sludge and a long time for the start-up of the process. Long, poor water quality during start-up
[0004] In the above studies, high-salt wastewater can significantly inhibit the activity of microbial treatment, resulting in the death of process sludge in normal operation and deterioration of effluent; the start-up process takes a long time and the removal rate is low during the start-up period

Method used

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  • Preparation method of salt-tolerant biological agent and adding method thereof
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Examples

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preparation example Construction

[0030] The preparation method of a salt-tolerant biological bacterial agent shown in the present invention, the salt-tolerant biological bacterial agent comprises a salt-tolerant high-efficiency bacterial agent and a salt-tolerant high-efficiency bacterial filler, and the salt-tolerant high-efficiency bacterial agent is prepared by the salt-tolerant bacteria O1 and the resistant bacteria. The salt-tolerant and high-efficiency bacterial filler is prepared by mixing and compounding the salt-resistant bacteria Y5. The salt-resistant and high-efficiency bacterial filler is made by immobilizing the salt-resistant high-efficiency bacterial agent and loading it on the polyurethane filler. , The salt-tolerant biological agent can treat high-salt wastewater through microbial decomposition. The salt-tolerant biological agent includes the salt-tolerant high-efficiency bacterial agent and the salt-tolerant high-efficiency bacterial filler. The salt-tolerant bacteria O1 and the salt-tolerant...

Embodiment 1

[0045] Example 1. The experiment records the effect of compound high-efficiency salt-tolerant bacteria with different ratios on the removal effect of organic pollutants in sewage, inoculated into domestic sewage with a salinity of 3%, and measured the removal effect of organic pollutants, so as to explore O1 and The optimal configuration ratio of two high-efficiency salt-tolerant bacteria in Y5;

[0046] The O1 and Y5 salt-tolerant bacteria suspensions were prepared into 107CFU / ML bacterial suspensions according to the ratios of 0 / 100, 25 / 75, 50 / 50, 75 / 25, and 100 / 0, respectively, and inoculated to a salinity of 3%. In the domestic sewage, shake culture at 25 °C and 150 r / min. The sampling period was 24 hours, and the samples were continuously sampled for 10 days. The samples were centrifuged and passed through a 0.5 micron membrane to determine the TOC value. Depend on figure 1 It can be seen from the experimental results that the maximum degradation rate of organic matter...

Embodiment 2

[0047] Embodiment 2. Use immobilization means to prepare salt-tolerant bacteria filler, prepare a salt-tolerant and efficient bacteria agent and salt-tolerant and efficient bacteria filler, wherein the salt-tolerant and efficient compound bacteria filler prepared by immobilization method can prolong the degradation of TOC by salt-tolerant and efficient bacteria The timeliness is divided into the following two aspects:

[0048] The O1 and Y5 salt-tolerant bacteria that have been screened in the laboratory were inoculated into LB liquid medium with salinity of 5% and 7%, respectively, and shaken at 25 ° C and 150 r / min, and the OD600 was measured every two hours. , when the OD600 values ​​of strains Y1 and Q5 reached 3.10 and 1.39, respectively, the bacterial concentration in the LB liquid medium could be measured to reach 107 CFU / ml by the line drawing method. The LB liquid medium containing the two strains of bacteria was mixed at 1:1, the supernatant was removed by centrifuga...

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Abstract

The invention relates to the field of sewage treatment, in particular to a preparation method of a salt-tolerant biological agent and an adding method thereof. The salt-tolerant biological agent comprises a salt-tolerant efficient inoculant and a salt-tolerant efficient bacteria filler, the salt-tolerant efficient inoculant is prepared by mixing and compounding halotolerant bacteria O1 and halotolerant bacteria Y5, according to the salt-tolerant efficient bacteria filler, the salt-tolerant efficient inoculant is immobilized, and loaded on a polyurethane filler. The influence of the composite efficient halotolerant bacteria in different proportions on the removal effect of organic pollutants in sewage is recorded through experiments, so that the optimal configuration proportion of the O1 efficient halotolerant bacteria and the Y5 efficient halotolerant bacteria is explored, and the treatment effect is greatly improved no matter whether the salt-tolerant efficient composite inoculant orthe salt-tolerant efficient composite microbial filler is added; and the salt-tolerant high-efficiency composite bacteria filler has an obvious effect of maintaining TOC degradation efficiency.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a preparation method and a dosing method of a salt-tolerant biological bacterial agent. Background technique [0002] High salinity wastewater refers to wastewater with a total salt content of ≥ 1%. In addition to a large amount of inorganic salts, this type of wastewater usually also contains organic matter and a total dissolved solids (TDS) with a mass fraction of ≥ 3.5%. High-salt wastewater can be divided into high-salt domestic sewage, high-salt industrial wastewater, and other types (domestic sewage and operational wastewater from large ships, salty water caused by seawater leakage, and concentrated water generated when wastewater is minimized). salt water. my country is a country with serious shortage of water resources, and the per capita water resources are only 2500m3. Therefore, in recent years, some coastal cities have actively carried out the direct use of seawater ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N11/08C02F3/34
CPCC12N1/00C12N11/08C02F3/34
Inventor 曾小芸
Owner 芜湖青弋环保科技有限责任公司
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