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Method for solidifying sand by utilizing halotolerant bacteria

A technology of bacteria and bacteria liquid, applied in the direction of bacteria, chemical instruments and methods, applications, etc., can solve the problems of difficult formation, no successful application cases, etc., and achieve the effect of simple operation and cost saving

Inactive Publication Date: 2015-12-30
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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Problems solved by technology

However, the naturally formed desert crust needs to be formed under very strict microclimate conditions, and it is very difficult to artificially control the formation of desert crust such as cyanobacteria. So far, there has been no successful application case

Method used

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  • Method for solidifying sand by utilizing halotolerant bacteria
  • Method for solidifying sand by utilizing halotolerant bacteria
  • Method for solidifying sand by utilizing halotolerant bacteria

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Embodiment Construction

[0014] A method for sand-fixing by salt-tolerant bacteria according to the present invention, in the presence of urea and calcium ions, the method uses desert underground salt water to fix the surface sand by mineralization, and the specific operations are carried out according to the following steps:

[0015] a. Bacteria source and culture: The bacteria selected are Bacillus sporosarcinapasteurii, the strain comes from the American Type Culture Collection, with the preservation number ATCC11859, the strain is placed in the nutrient broth medium, and after recovery, transfer to 50ml nutrient broth for culture In the base conical flask, the pH was adjusted to 4-11, and then the conical flask was placed under the conditions of a rotating speed of 130 rpm and a temperature of 30°C for 48 hours to obtain the acclimatized and enriched bacterial liquid;

[0016] b. Preparation of bacterium liquid with inductive solidification effect: the bacterial bacterium liquid obtained in step a ...

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Abstract

The invention relates to a method for solidifying sand by utilizing halotolerant bacteria. The method selects Sporosarcina pasteurii as bacteria, the strain comes from American Type Culture Collection (ATCC), and the collection number is ATCC 11859. The method comprises the following steps: putting the strain in a nutrient broth medium to obtain a bacterial solution after domestication and enrichment; putting the bacterial solution in desert underground brine containing urea, calcium ions and salinity, and culturing to obtain a bacterial solution with an induced solidification effect; spraying the bacterial solution on the surface of sand soil; putting the sand soil of which the surface is sprayed with the bacterial solution in the natural drying environment for culturing; and naturally air-drying, so that the bacteria can utilize the desert underground brine to cement and solidify sand grains on the surface of the sand soil through the solidification effect so as to prevent the sand soil on the surface layer from migrating under the wind effect. The method is environment-friendly, is low in cost, and can be widely used for solidifying mobile and semi-mobile dunes.

Description

technical field [0001] The invention relates to a method for immobilizing movable sand dunes by using desert underground salt water by salt-tolerant bacteria with curing function, and belongs to the technical field of biological environment engineering. Background technique [0002] Land desertification refers to the degradation of land in arid, semi-arid and sub-humid areas with drought disasters due to factors such as climate change and unreasonable human economic activities, that is, land degradation, also known as "desertification". Among the many environmental problems of human beings, desertification is one of the most serious disasters. The global sandy soil is expanding at a rate of 50,000 to 70,000 square kilometers per year. More than 1 billion people and more than 40% of the land surface are affected by desertification. . With the gradual aggravation of desertification, the number of sandstorms in northern my country is increasing year by year. In March and Apri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E02D3/12C12N1/20C09K17/00C09K105/00
Inventor 潘响亮陈飞宋文娟
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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