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High-salt-tolerant bacterium and application thereof

A technology of salt-tolerant bacteria and high sulfate, applied in the direction of bacteria, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problem of no investigation of strain stability and long-term adaptability, lack of implementation details, sulfate quality Low proportion and other issues, to achieve the effects of high efficiency recovery, strong stability, and excellent salt tolerance

Active Publication Date: 2022-07-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the patents in this direction mostly use NaCl as the main component of salt in strain screening and cultivation, and the mass ratio of sulfate is relatively low. This is because when the concentration of sulfate in the medium / liquid is high, S 2- Or hydrogen sulfide, which brings certain biological toxicity, inhibits or kills bacteria
Moreover, the salt-tolerant bacteria involved in the above-mentioned patents only briefly stated that they can be used in high-salt wastewater, and did not examine the stability and long-term adaptability of the strains in special environments, and lacked specific implementation details, and the saline wastewater was Wastewater containing NaCl

Method used

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  • High-salt-tolerant bacterium and application thereof
  • High-salt-tolerant bacterium and application thereof
  • High-salt-tolerant bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The screening process of the high salt-tolerant bacteria GXNYJ-DL-1 strain provided by the invention is as follows:

[0055] (1) Sampling: Take activated sludge and sewage samples from the binary acid sewage treatment plant of Qingjiang Petrochemical in Huaian City, Jiangsu Province, and store them in a refrigerator at 4°C for future use.

[0056] (2) Sludge activation: take the spare sewage and activated sludge in the refrigerator and put them into a reactor equipped with an aeration device for 48-72 hours, and control the dissolved oxygen to be ≥2mg / L.

[0057] (3) Initial acclimation: After the suffocating exposure, take 10 mL of the mixed bacterial solution and put it into a conical flask with 200 mL of liquid culture medium, and then put it into a shaker for cultivation. The culture period is 48h to 72h. After the culture, 10ml of bacterial liquid is taken and placed in the second batch of liquid medium, and so on; the salt content of the first liquid medium is 1% ...

Embodiment 2

[0062] Identification of strains:

[0063] Through physiological and biochemical identification and 16S rDNA gene sequencing analysis, the results of physiological and biochemical identification are shown in Table 1, and the results of gene sequence determination are shown in the sequence table.

[0064] Table 1

[0065]

[0066] Note: "+" indicates positive reaction or available; "-" indicates negative reaction or unavailable.

Embodiment 3

[0068] Determination of salt tolerance of highly salt-tolerant bacteria GXNYJ-DL-1

[0069] Preparation of simulated wastewater (g / L): phenol 0.4, NaCl 3, FeSO 40.02, CaCl 2 0.03, MgSO 4 1, Na 2 SO 4 3. KH 2 PO 4 0.034, NH 4 Cl 0.3, yeast extract 0.1, tryptone 0.05g, pH 7, salt content about 1% (mass percentage). Addition of Na on the basis of simulated wastewater with 1% salt content 2 SO 4 The wastewater with salt content of 5%, 9%, 13%, 17%, 21% and 25% was prepared.

[0070] Take the GXNYJ-DL-1 bacterial solution, add it to the conical flask according to the volume ratio of 1:20 with the simulated wastewater, use the shaking table shaking method, control the temperature at 35 ° C, the rotation speed is 150 rpm, take regular samples, and use a spectrophotometer to measure the bacteria. Density (OD 600 ), plot the growth curve of the strain, the growth curve of the strain under different salt concentrations, see image 3 ; And determine the COD value of the fi...

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Abstract

The invention discloses a strain of highly halotolerant bacteria (Halomonas nigrifans) GXNYJ-DL-1, which has been preserved in China General Microbiological Culture Collection Center (CGMCC) on July 13, 2020, and the preservation number is CGMCC No.20350. The invention also discloses a method for preparing the highly halotolerant bacteria (Halomonas nigrifans) GXNYJ-DL-1). The form of the high-halotolerant bacterium GXNYJ-DL-1 is as follows: a bacterial colony is light yellow and round, the surface is wet and opaque, and the edge is neat; the micromorphology under a microscope is as follows: thalli are rod-shaped, 0.5-0.7 [mu] m * 1.2-3.6 [mu] m, are arranged in a single or paired manner, and are gram negative. The high-salt-tolerant bacterium GXNYJ-DL-1 disclosed by the invention is excellent in salt tolerance, particularly can tolerate high sulfate, and is more suitable for removing COD (Chemical Oxygen Demand) of high-salt wastewater, particularly high-sulfate wastewater compared with a high-salt-tolerant strain in the prior art.

Description

technical field [0001] The invention relates to a high salt-tolerant bacterium and its application in removing COD from high-salt wastewater, belonging to the technical field of microorganisms and wastewater treatment. Background technique [0002] Chemical, pharmaceutical, papermaking, food processing, mining, fermentation and other industries are prone to produce high-salt wastewater, especially high-sulfate organic wastewater. Due to the existence of a large number of sulfate ions, this high-salt wastewater is not easy to biochemically, and due to the high organic matter, it is neither economical nor desirable (easy to block) to use physical and chemical process routes such as membrane separation and concentration crystallization. [0003] For this kind of wastewater, the existing methods and research directions are as follows: [0004] (1) Chemical method, that is, adding lime to convert sulfate into calcium sulfate precipitation. Such as patents CN106865880A, CN105439...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/30C12R1/01C02F101/10
CPCC12N1/20C02F3/34C02F3/301C02F2101/101
Inventor 马和旭秦中良卢利玲桂秋芬汪永春邓怀林程晓东程梦婷
Owner CHINA PETROLEUM & CHEM CORP
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