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Solid fermentation inoculum of chicken-origin bacillus subtilis and pediococcus pentosaceus and preparation method and application of solid fermentation inoculum

A technology of Bacillus subtilis and Pediococcus pentosaceus, which is applied in the field of microorganisms, can solve the problems of low bulk density and limited use ratio, and achieve the effects of low cost, easy absorption, and maintaining the health of intestinal microecology

Active Publication Date: 2013-02-20
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The main raw materials of poultry feed mainly include corn flour and soybean meal; wheat bran has a high protein content, and its amino acid composition can be compared with that of whole grain wheat, so it also occupies a certain proportion in poultry feed; but its high fiber, low bulk density, etc. The feature limits its usage ratio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preferable strains: Bacillus subtilis SCS4562 and Pediococcus pentosaceae SCS4560 of the present invention are isolated from the cecum of healthy chickens, and the Bacillus subtilis SCS4562 strain has been preserved in the China Microbiological Culture Collection on September 14, 2012 General Microbiology Center of the Management Committee, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, deposit number: CGMCC NO.6565, classification name: Bacillus subtilis;

[0029] Pediococcus pentosaceus (Pediococcus pentosaceus) SCS4560 strain has been preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on September 14, 2012. The address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Preservation number: CGMCC NO.6566, classification name: Pediococcus pentosaceus;

[0030] (2) Arti...

Embodiment 2

[0055] (1) The bacterial classification is the same as in Example 1

[0056] (2) Seed culture: Put the strain described in (1) under sterile conditions, connect 1~2 rings with the inoculation loop into 40ml liquid seed medium, and cultivate it at 120 rpm, 37°C constant temperature shaker for 48h , to obtain the first-grade seed solution;

[0057] (3) Connect 2 to 3 loops with the inoculation loop in the liquid seed medium of the same formula, and cultivate it on a constant temperature shaker at 37°C for 36 hours at a speed of 120 rpm to obtain a secondary seed liquid.

[0058] (4) Solid fermentation culture:

[0059]The secondary seed liquid of Bacillus subtilis was inoculated in a solid medium sterilized at 121° C. for 30 minutes at a mass ratio of 5%, and inoculated according to the material-water ratio of 1:1.1 g / ml and the volume ratio of 16%. Put it into a fermentation bottle, mix it and put it in a biochemical incubator, and use a 4-layer sterilized gauze bottle mouth,...

Embodiment 3

[0070] (1) The strains are the same as in Example 1.

[0071] (2) Seed culture: Put the strain described in step (1) under aseptic conditions into 40ml liquid seed culture medium with an inoculation loop and inoculate 1 to 2 loops, and culture it on a constant temperature shaker at 120 rpm at 37°C 48h, get the first grade seed solution;

[0072] (3) Connect 2 to 3 loops with the inoculation loop in the liquid seed medium of the same formula, and cultivate it on a constant temperature shaker at 37°C for 36 hours at a speed of 120 rpm to obtain a secondary seed liquid.

[0073] (4) Solid fermentation culture:

[0074] Inoculate the Bacillus subtilis described in step (1) with an inoculation amount of 5% mass ratio, and inoculate the secondary seed liquid in a solid medium sterilized at 121°C for 30 minutes, according to the ratio of material to water: 1: 1.1 g / ml and a volume ratio of 16% into the fermentation bottle, mixed and placed in a biochemical incubator, and with a 4-...

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Abstract

The invention discloses a method for preparing a solid fermentation inoculum of chicken-origin bacillus subtilis and pediococcus pentosaceus. The method comprises the steps of selecting a bacillus subtilis SCS4562 bacterial strain and a pediococcus pentosaceus SCS4560 bacterial strain, wherein, the preservation number of the bacillus subtilis SCS4562 bacterial strain is CGMCC NO.6565, and the preservation number of the pediococcus pentosaceus SCS4560 bacterial strain is CGMCC NO.6566; and obtaining a fermentation product which is the solid fermentation inoculum through fermentation of the two bacterial strains. The solid fermentation inoculum is added into feed directly, the intestinal micro-ecological health is maintained, the solid fermentation inoculum helps intestinal canals to absorb nutrients in the feed, and the growth performance of chickens is improved.

Description

technical field [0001] The invention relates to a solid fermentation bacterial agent of chicken-derived Bacillus subtilis and Pediococcus pentosacea, a preparation method and application thereof, and belongs to the technical field of microorganisms. Background technique [0002] Bacillus subtilis (Bacillus subtilis) belongs to the genus Bacillus, rod-shaped, evenly stained; spores 0.8×1.5~1.8μm; colony source or irregular shape, dark surface; can wrinkle, forming cream or brown; in the culture medium A complete film can be formed in medium, slightly turbid. [0003] Pediococcus pentosaceus (Pediococcus pentosaceus) belongs to the Pediococcus book, spherical, quadruple live into a sheet-like arrangement; the cell diameter is about 1 μm; it grows well on the MRS medium, and the colonies growing on the surface are regular round, white and flat; It is turbid in the liquid medium, and the bacteria settle to the bottom of the medium and appear white. [0004] Pediococcus pentosa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20A23K1/16C12R1/01C12R1/125
Inventor 刘益平孙静李念珍熊彦朱庆钟航
Owner SICHUAN AGRI UNIV
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