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Acid soil conditioner and its preparation method and application

A technology of acidic soil and improver, applied in application, soil conditioning materials, chemical instruments and methods, etc., to achieve the effect of less raw material consumption, simple preparation process and low cost

Active Publication Date: 2021-10-08
GUANGXI JINSUI ECOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no precedent for anyone to use Alcaligenes faecalis to make an acidic soil amendment

Method used

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  • Acid soil conditioner and its preparation method and application
  • Acid soil conditioner and its preparation method and application
  • Acid soil conditioner and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] An acidic soil improver, the total bacterial count of Alcaligenes faecalis in every 1 milliliter of the acidic soil improver is greater than or equal to 5 billion.

[0030] A preparation method of an acidic soil conditioner, comprising the following steps:

[0031] Step 1: Obtain the original species of Alcaligenes faecalis, and after thawing, separate them by streaking on an NA medium plate, and culture them at 35° C. for 24 hours to obtain a single colony of Alcaligenes faecalis.

[0032] Step 2: pick a single colony of Alcaligenes faecalis and inoculate it in NB liquid medium, culture it on a shaker at 35° C. and 200 rpm for 20 hours to obtain an activated seed solution.

[0033] Step 3: Take the activated seed solution at a volume ratio of 2% and transfer it to the liquid fermentation medium, and expand the cultivation at 35° C. and 200 rpm on a shaker for 20 hours to obtain a large amount of seed solution.

[0034] Step 4. Transfer the large amount of seed liquid ...

Embodiment 2

[0036] An acidic soil improver, the total bacterial count of Alcaligenes faecalis in every 1 milliliter of the acidic soil improver is greater than or equal to 5 billion.

[0037] A preparation method of an acidic soil conditioner, comprising the following steps:

[0038] Step 1: Obtain the original species of Alcaligenes faecalis, and after thawing, separate them by streaking on an NA medium plate, and culture them at 37° C. for 24 hours to obtain a single colony of Alcaligenes faecalis.

[0039] Step 2: pick a single colony of Alcaligenes faecalis and inoculate it in NB liquid medium, culture it on a shaker at 37° C. and 200 rpm for 18 hours to obtain an activated seed solution.

[0040] Step 3: Take the activated seed solution at a volume ratio of 1% and transfer it to the liquid fermentation medium, and expand the cultivation at 37° C. and 200 rpm on a shaker for 18 hours to obtain a large amount of seed solution.

[0041] Step 4: transfer the large amount of seed liquid ...

Embodiment 3

[0043] An acidic soil improver, the total bacterial count of Alcaligenes faecalis in every 1 milliliter of the acidic soil improver is greater than or equal to 5 billion.

[0044] A preparation method of an acidic soil conditioner, comprising the following steps:

[0045] Step 1: Obtain the original species of Alcaligenes faecalis, and after thawing, separate them by streaking on an NA medium plate, and culture them at 35° C. for 24 hours to obtain a single colony of Alcaligenes faecalis.

[0046] Step 2: pick a single colony of Alcaligenes faecalis and inoculate it in NB liquid medium, culture it on a shaker at 32° C. and 200 rpm for 36 hours to obtain an activated seed solution.

[0047]Step 3: Take the activated seed solution according to the volume ratio of 4% and transfer it to the liquid fermentation medium, and expand the cultivation at 32° C. and 200 rpm on a shaking table for 24 hours to obtain a large amount of seed solution.

[0048] Step 4: Transfer the large amou...

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Abstract

The invention discloses an acidic soil improver. The total bacteria quantity of Alcaligenes faecalis in every 1 milliliter of the acidic soil improver is greater than or equal to 5 billion. The invention also discloses the preparation method and application of the acidic soil improver. The soil improver of the present invention uses live Alcaligenes faecalis bacteria as functional bacteria. Through the life activities of the bacteria, it can not only effectively increase the pH value of the acidic soil, reduce the EC value of the soil, inhibit the growth and reproduction of pathogenic bacteria, and improve the pH value of the soil. The structure of the microbial community enables the soil to release fixed nutrients and fundamentally improve the soil without causing damage to the ecological environment.

Description

technical field [0001] The invention belongs to the technical field of soil improvement, and in particular relates to an acidic soil improver and its preparation method and application. Background technique [0002] Acidic soil is a general term for soil with a pH value less than 6.5, including brick red soil, red soil, red soil, yellow soil and dry red soil, which are mainly distributed in tropical and subtropical regions in my country. Mainly due to the large and concentrated precipitation, the strong leaching effect, and the loss of alkaline bases such as calcium, magnesium, and potassium, lead to soil acidification. The application of lime, fire dung and large amount of chemical fertilizers in traditional agriculture are also important causes of soil acidification. The hazards of soil acidification mainly include the following points: First, soil acidification will reduce the number of beneficial microorganisms in the soil, inhibit the growth and activities of beneficia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/14C09K17/40C09K101/00C09K109/00
CPCC09K17/14C09K17/40C09K2101/00C09K2109/00
Inventor 马金霞谭寿湖张琦敏林振业梁春吉
Owner GUANGXI JINSUI ECOLOGICAL TECH CO LTD
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