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Odorless improved microbial agent for kitchen waste treatment and its preparation method and application

A technology for microbial inoculants and kitchen waste, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc. To avoid the reduction of the number of viable bacteria, reduce the number of inactivated bacteria, and enhance the protective effect

Active Publication Date: 2020-09-29
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above-mentioned patent applications all provide the microbial strain formula of the food waste degrading bacteria agent, none of the above-mentioned strains involves the problem of eliminating the peculiar smell in the process of degrading the food waste, resulting in a very obvious odor of the degradation product
[0007] In addition, some patent applications use the carrier as an immobilized material in the preparation method of the bacterial agent to improve the stability and shelf life of the bacterial agent; however, most of the carriers will have caking problems after they are mixed with the bacteria and dried. , so mechanical crushing is required when the bacterial agent is used, and the process of mechanical crushing will inactivate part of the bacteria, thereby reducing the number of viable bacteria in the bacterial agent

Method used

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  • Odorless improved microbial agent for kitchen waste treatment and its preparation method and application
  • Odorless improved microbial agent for kitchen waste treatment and its preparation method and application
  • Odorless improved microbial agent for kitchen waste treatment and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Example 1 Screening and Identification of Predominant Bacteria for Degrading Food Waste

[0048] Take 5g of the soil sample near the Yuxiu Restaurant of Zhejiang University of Technology and put it into a sterilized Erlenmeyer flask with small glass beads and 50mL of sterile water, shake at 30°C and 200r / min for 20min, and suspend the bacteria in the ultra-clean bench. Liquid gradient dilution, the concentration gradient is 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 , inoculate 100 μL of each concentration of bacterial suspension on a PDA plate, seal the plate with a sealing film and invert it, place it in a constant temperature incubator at 28-37°C for 24-48 hours, select a plate with a suitable number of colonies, and pick typical colonies , after separation and purification, inoculate them on protein, starch, fat, and cellulose differential medium plates and spread evenly with a sterilized coating rod. Seal the culture dish with a parafilm and place it ups...

Embodiment 2

[0072] The preparation of embodiment 2 microbial bacterial agents

[0073] 1. Preparation of unimproved microbial agent

[0074] (1) Preparation of composite bacteria

[0075] (1) Incline cultivation:

[0076] Pichia kluyveri ZJB-091 and Geotrichumsilvicola ZJB-092 were inoculated on PDA solid medium respectively;

[0077] Among them, Geotrichum linsheng was cultured at a temperature of 30° C. for 48 hours, and Pichia kluyveri was cultured at a temperature of 28° C. for 72 hours to activate the strains.

[0078] (2) Primary seed liquid culture:

[0079] The activated Pichia kluyveri and Geotrichum forestiata after the activation of step (1) are inoculated respectively in 250mL liquid culture medium, carry out seed culture, obtain Pichia kluyveri first grade seed liquid culture and Geotrichum forestiata grade one seed liquid culture;

[0080] Among them, Geotrichum linsheng was cultured at a temperature of 30° C. for 48 hours, and Pichia kluyveri was cultured at a temperat...

Embodiment 3

[0114] This embodiment is to use the different microbial bacterial agents obtained in Example 2 to carry out degradation tests on kitchen waste:

[0115] (1) Take the leftover meals in the school cafeteria and sort them to remove solid waste such as bones, paper scraps, wooden chopsticks, and plastic;

[0116] (2) Add 15kg of sorted kitchen waste in the garbage disposer, get the microbial inoculum that embodiment 2 makes (respectively unimproved microbial inoculum, odorless type improved microbial inoculum I and odorless type The improved microbial agent II) 900g is put into the garbage disposer, and after 24 hours of continuous fermentation, 10kg of the above-mentioned food waste is added every other day for 10 consecutive days. The weight ratio of the total food waste added to the microbial agent is about 130:1;

[0117] The fermentation temperature is 55°C, and the fermentation time is 28 hours; during the fermentation process, stirring is carried out, and the stirring fre...

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Abstract

The invention discloses an odorless type improved microbial agent for kitchen waste processing and a preparation method and application thereof. The odorless type improved microbial agent for kitchen waste processing is prepared from components: 5-15 parts of a compound bacterium body, 50-150 parts of a carrier, 1-3 parts of a binding agent and 1-5 parts of a protective agent. The compound bacterium body is formed by mixing pichia kluyveri (pk) ZJB-091 and geotrichum silvicola (gs) ZJB-092 with the mass ratio of (1-15) to (0.5-10). The carrier is wheat straw. According to the odorless type improved microbial agent for kitchen waste processing and the preparation method and application thereof, the pichia kluyveri and the geotrichum silvicola are used as the compound bacterium body, the wheat straw is used as the carrier, the obtained microbial agent has obviously enhanced kitchen waste degrading capacity, peculiar smell generated in the process of degrading the kitchen waste can be removed, microbial agent agglomeration is prevented, and thus the problem of reduced viable count caused by mechanical pulverization in the microbial agent using process is solved.

Description

technical field [0001] The invention relates to the field of environmental protection biotechnology, in particular to an odorless improved microbial agent for treating kitchen waste and its preparation method and application. Background technique [0002] With the development of our country's social economy and the improvement of people's living standards, the amount of kitchen waste is increasing rapidly; if these kitchen waste is not properly treated, it will become a public nuisance to society. Since food waste is easy to ferment, deteriorate and rot, if it is directly discharged into the sewer in large quantities, it will produce a large amount of toxins, emit foul-smelling gases, and pollute water bodies and the atmosphere. The "soil oil" or "gutter oil" derived from kitchen waste contains carcinogens - aflatoxin and other toxins, which are extremely harmful to human health. Since 2011, it has attracted the attention of the public. Many cities have actively legislated ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/12C12N11/10C12N11/084B09B3/00C12R1/84C12R1/645
CPCB09B3/00C12N11/08C12N11/10C12N11/12
Inventor 薛亚平刘奇郑裕国邹树平柯霞周海岩柳志强
Owner ZHEJIANG UNIV OF TECH
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