Composite bacillus subtilis bio-organic fertilizer and preparation method thereof
A bio-organic fertilizer and Bacillus subtilis technology, applied in biochemical equipment and methods, methods based on microorganisms, bio-organic part treatment, etc., can solve the problems of low number of active bacteria and cumbersome preparation methods, and achieve simple preparation methods, Improved disease resistance, simple operation
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Embodiment 1
[0027] see figure 1 The present invention provides a technical solution: a compound Bacillus subtilis bio-organic fertilizer, including organic matter and complex flora, the complex flora includes at least two types of Bacillus subtilis, yeast and actinomycetes, and the organic matter includes crop straw, One or more of organic waste, animal manure, and agricultural product waste.
[0028] The composite flora includes bacillus subtilis and actinomycetes, and the mixing ratio of bacillus subtilis and yeast is 2:1 according to the mass.
[0029] A preparation method of compound type Bacillus subtilis bio-organic fertilizer, the specific steps are as follows:
[0030] S1. Preparation of bacterial solution: inject the compound bacterial group into the petri dish, and add the culture solution at the same time;
[0031] S2. Bacterial strain culture: cultured under sterile conditions, the culture temperature is 30°C, and the pH value of the culture environment is 7.2;
[0032] S3,...
Embodiment 2
[0041] see figure 1 The present invention provides a technical solution: a compound Bacillus subtilis bio-organic fertilizer, including organic matter and complex flora, the complex flora includes at least two types of Bacillus subtilis, yeast and actinomycetes, and the organic matter includes crop straw, One or more of organic waste, animal manure, and agricultural product waste.
[0042] The compound flora includes Bacillus subtilis and yeast, and the mixing ratio of Bacillus subtilis and yeast is 2:1 according to mass.
[0043] A preparation method of compound type Bacillus subtilis bio-organic fertilizer, the specific steps are as follows:
[0044] S1. Preparation of bacterial solution: inject the compound bacterial group into the petri dish, and add the culture solution at the same time;
[0045] S2. Bacterial strain culture: cultured under sterile conditions, the culture temperature is 38°C, and the pH value of the culture environment is 7.5;
[0046] S3, check the nu...
Embodiment 3
[0055] see figure 1 The present invention provides a technical solution: a compound Bacillus subtilis bio-organic fertilizer, including organic matter and complex flora, the complex flora includes at least two types of Bacillus subtilis, yeast and actinomycetes, and the organic matter includes crop straw, One or more of organic waste, animal manure, and agricultural product waste.
[0056] The composite flora includes Bacillus subtilis, yeast and actinomycetes, and the installation quality mixing ratio of Bacillus subtilis, yeast and actinomycetes is: 2:1:1.
[0057] A preparation method of compound type Bacillus subtilis bio-organic fertilizer, the specific steps are as follows:
[0058] S1. Preparation of bacterial solution: inject the compound bacterial group into the petri dish, and add the culture solution at the same time;
[0059] S2. Bacterial strain culture: cultured under sterile conditions, the culture temperature is 37°C, and the pH value of the culture environme...
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