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Compound biological deodorizer as well as preparation method and application thereof

A biological deodorant and seed technology, applied in biochemical equipment and methods, microorganism-based methods, deodorization, etc., can solve the problems of deodorization effect affected by environmental conditions, short action time, and increased deodorization cost, etc. To achieve the effect of benefiting the environment, eliminating or odor generation, and reducing odor concentration

Active Publication Date: 2015-04-29
北京沃土天地生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, most domestic researchers have focused on using a single method, and combined multiple methods, such as combining plant extracts with microorganisms in a certain way to form a compound biological deodorant, which is rarely reported in practice.
[0007] The main problems of deodorants in the existing market are: first, the action time is short, and a large amount of deodorants need to be consumed in a short period of time, which increases the cost of deodorants; second, the applicability of deodorants is poor, and the deodorant effect is affected by environmental conditions Great instability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 composite biological deodorant

[0046] 1. Composition:

[0047] plant extracts;

[0048] Pichia pastoris J6 (Saccharomycetes), 3.9×10 7 -7.2×10 7 cfu / mL;

[0049] Pediococcus pentosaceus FJ-13 (Pediococcus pentosaceus), 2.9×10 8 -3.6×10 8 cfu / mL;

[0050] Pediococcus acidilactici FJ-18 (Pediococcus acidilactici) 3.9×10 8 -4.6×10 8 cfu / mL;

[0051] Candida FJ-22 (Candida mycoderma), 4.7×10 7 -8.2×10 7 cfu / mL;

[0052] Bacillus megatherium LS-1 (Bacillus megatherium) 8×10 6 -1×10 7 cfu / mL;

[0053] The total number of bacteria is 7.6×10 8 -9.84×10 8 cfu / mL.

[0054] 2. Preparation method:

[0055] 1) Dilute the stock solution of the plant extract 100 times with sterile water to obtain solution A, which is set aside.

[0056] 2) Solid slant culture: Bacillus megaterium LS-1 was inoculated in beef extract peptone solid medium, Pichia pastoris J6 was inoculated in potato solid medium, Pediococcus pentosaceae FJ-13 and Pediococcus lactis FJ-18...

experiment example 1

[0063] Experimental example 1 Deodorizing effect test of compound biological deodorant on fresh pig manure

[0064] The deodorizing effect of fresh pig manure is measured, and the specific detection method is as follows:

[0065] 1. Test samples: fresh pig manure, compound biological deodorant, water, deodorant product A already available on the market.

[0066] 2. Detection method: put 50g of fresh pig manure mixed with 25% sawdust into a 1000mL beaker, adjust the water content to about 50%, sterilize at 121°C for 30min, add compound biological deodorant according to 10% inoculation amount, water And the existing deodorizing product A on the market, and fully mix, respectively put a 50mL small beaker containing 20mL dilute sulfuric acid solution and a 50mL small beaker containing 20mL zinc acetate-sodium acetate solution in the cup. After sealing with a double-layer plastic film, place it in a constant temperature room at 30°C for fermentation and cultivation. Each treatmen...

experiment example 2

[0071] Experimental example 2 Deodorization effect of compound biological deodorant on garbage transfer station

[0072] Applied to a garbage transfer station in Beijing to measure the deodorization effect, the specific method is:

[0073] 1. Test samples: fresh domestic waste at transfer station; compound biological deodorant; control: water; deodorant product A already on the market.

[0074] 2. Test method: Dilute the deodorizing microbial compound bacterial agent 100 times with water, press 0.1Kg / m 2 Spray on all occasions. Sampling tool has NH 3 、H 2 S absorption meter and atmospheric sampler to record the temperature, atmospheric pressure and humidity during sampling. The samples were sent to Beijing for determination by the Livestock and Poultry Breeding Environmental Monitoring Center, and the NH 3 and H 2 S content.

[0075] 3. Through continuous 15d sampling analysis, the results are shown in Table 2

[0076] Table 2: Deodorization effect on garbage transfer ...

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Abstract

The invention relates to a compound biological deodorizer as well as a preparation method and application thereof. The compound biological deodorizer comprises a plant extract, pichia pastoris, pediococcus pentosaceus, pediococcus acidilactici, candida mycoderma and bacillus megatherium. Through the utilization of compound biological deodorizer to treat a waste transfer station, a livestock and poultry farm and a composting site, the NH3 removal rate can respectively reach 65.2-71.6%, 63.4-73.7% and 53.42-75.77%, and the H2S removal rate can reach 53.5-60.1%, 35.5-45.9% and 47.2-53.8%, so that the odor concentration is obviously reduced, the environment of the periphery of the waste transfer station, the livestock and poultry farm and the composting site is effectively improved, and the improvement of both economic benefits and environment-friendly benefits is facilitated.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a composite biological deodorant and its preparation method and application. Background technique [0002] With the development of social economy, people's requirements for the environment in which they live are also increasing. As an important environmental pollutant and one of the seven typical public hazards (air pollution, stench, noise, water pollution, vibration, soil pollution and foundation subsidence), stench has a wide variety of substances and a wide range of influence. It has also gradually gained people's attention. Large-scale garbage transfer stations, livestock and poultry farms and composting yards are important sources of odor. Therefore, how to develop highly efficient, sustainable, non-polluting deodorants has become a hot topic of current research. [0003] At present, conventional deodorization methods include: physical deodorization method and chem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L9/013C12N1/20C12N1/14C12N1/16A61L101/52C12R1/84C12R1/11C12R1/01
Inventor 杨军任莉彭生平
Owner 北京沃土天地生物科技股份有限公司
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