A strain of Weissella enterica-like and its application

A Weissella enteric membrane technology, applied in the field of microorganisms, can solve the problem of lack of Weissella entericoids, etc., and achieve the effects of reducing diarrhea rate, fast growth, and strong acid production

Active Publication Date: 2021-05-25
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is a lack of a strong bacteriostatic and acid-producing Weissella enterica and its application

Method used

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  • A strain of Weissella enterica-like and its application
  • A strain of Weissella enterica-like and its application
  • A strain of Weissella enterica-like and its application

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Experimental program
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Effect test

Embodiment 1

[0031] A strain of Weissella enteroides of the present invention, the strain is Weissella paramesenteroides; the preserved name is Weissella paramesenteroides LL2018353; it is preserved in the General Microorganism Center of China Microbiological Cultures Preservation Committee ( CGMCC), the deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing; date of deposit: October 21, 2019; deposit number: CGMCC NO.18710.

[0032] The 16S rDNA gene sequence of Weissella enteroides is the nucleotide sequence shown in SEQ ID No.1.

[0033] In MRS agar medium, after 72 hours of aerobic culture at 35°C, the colony is milky white, round, smooth in surface, neat in edge, convex in the middle, without pigmentation, easy to stir up, without silk, and has the smell of lactic acid. No pungent sour smell; Gram staining is positive, the bacteria are rod-shaped, appearing singly or in pairs, facultative anaerobic, and the optimum growth temperature is 38°C.

[0034...

Embodiment 2

[0046] The difference between embodiment 2 and embodiment 1 is:

[0047] In MRS agar medium, after 72 hours of aerobic culture at 30°C, the colony is milky white, round, smooth in surface, neat in edge, convex in the middle, without pigmentation, easy to stir up, without silk, and has the smell of lactic acid. No pungent sour smell; Gram staining is positive, the bacteria are rod-shaped, appearing singly or in pairs, facultative anaerobic, and the growth temperature is 30°C.

[0048] The preparation method of Weissella enterica bacterium agent of the present invention comprises the following steps:

[0049] (1) Strain preparation: Inoculate in MRS liquid medium, culture at 30° C. for 24 hours, and centrifuge at 8000 rpm to obtain bacterial cells.

[0050] (2) Freeze-dry the cells.

[0051] The effective number of viable bacteria of the thalline in the microbial preparation is 10 10 CFU / g.

Embodiment 3

[0053] The difference between embodiment 3 and embodiment 1 is:

[0054] In MRS agar medium, after 72 hours of aerobic culture at 35°C, the colony is milky white, round, smooth in surface, neat in edge, convex in the middle, without pigmentation, easy to stir up, without silk, and has the smell of lactic acid. No pungent sour smell; Gram staining is positive, the bacteria are rod-shaped, appearing singly or in pairs, facultatively anaerobic, and the growth temperature is 35°C.

[0055] The preparation method of Weissella enterica bacterium agent of the present invention comprises the following steps:

[0056] (1) Strain preparation: Inoculate in MRS liquid medium, culture at 35° C. for 20 h, and centrifuge at 6000 rpm to obtain bacterial cells.

[0057] (2) Freeze-dry the cells.

[0058] The effective number of viable bacteria of the thalline in the microbial preparation is 10 11 CFU / g.

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Abstract

The invention discloses a strain of Weissella enterica-like and its application. A strain of Weissella enteroides of the present invention, the strain is Weissella paramesenteroides; the preservation name is Weissella enteritidis LL2018353; it is preserved in China Microorganism Culture Preservation Management Committee Common Microorganisms Center (CGMCC), the deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing; date of deposit: October 21, 2019; deposit number: CGMCC NO.18710. The 16S rDNA gene sequence of Weissella enteroides is the nucleotide sequence shown in SEQ ID No.1. The invention has strong bacteriostasis and acid production, can be stored at low temperature (4 degrees Celsius) for a long time, and can reduce the diarrhea rate and constipation rate of pigs.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Weissella enterica-like and its application. Background technique [0002] Lactic acid bacteria is one of the feed microbial additives popularized in recent years. It can use carbohydrate fermentation to produce organic acids and other substances. Research data show that lactic acid bacteria can promote animal growth, regulate intestinal pH, maintain intestinal micro-ecological balance, thereby improving gastrointestinal function, increasing digestibility and biological potency, inhibiting the growth of spoilage bacteria in the intestinal tract, and improving the body's immunity. At present, most of the lactic acid bacteria on the market grow anaerobically and have a single variety, which cannot meet the actual market demand. Weissella enteroides is a lactic acid bacterium discovered this year. It mainly exists in kimchi and is used in the fermentation of kim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30A23L33/135A61K35/744A61P31/04C12R1/01
CPCA23V2002/00A61K35/744A23K10/18A23K50/30A23L33/135A61P31/04C12N1/20C12N1/205C12R2001/01A23V2200/30
Inventor 刘韶娜赵彦光赵智勇张斌相德才
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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