Preparation technology of bacillus feed additive agent

A technology for feed additives and Bacillus, which is applied in the field of preparation of Bacillus feed additives, can solve the problems of limiting the wide application of microecological preparations, affecting the application effect, high product price, etc., and achieves good inhibition of pathogenic bacteria, stable application effect and good resistance. Inverse performance effect

Active Publication Date: 2011-11-09
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the following major quality problems exist in the probiotics on the market: (1) low live bacteria content; (2) high moisture content, which is one of the important factors affecting the quality of probiotics; (3) Intolerant to antibiotics; (4) Unstable to gastric acid and bile salts, so that only a few strains are still active after entering the intestinal tract, and the number of viable bacteria required to exert their effects cannot be reached
Due to these quality problems of probiotics, it affects its application effect on animals and limits the wide application of probiotics
Microencapsulation method can increase the number of live bacteria in the product, but the product obtained by this method is expensive

Method used

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  • Preparation technology of bacillus feed additive agent
  • Preparation technology of bacillus feed additive agent
  • Preparation technology of bacillus feed additive agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) inoculating the strains of Bacillus licheniformis into BPY seed medium for activation, culturing at 200 rpm at 37°C for 18 hours to obtain a culture with a spore rate above 95%;

[0021] 2) Determination of acid resistance: inoculate the cultures prepared above at 5% inoculum size into artificial simulated gastric juices with pH values ​​of 2.0, 3.0, and 4.0, count at 0h as a control, and use phosphate buffer for sampling at 2h and 6h in a 10-fold series Dilute, count the viable bacteria on the plate, and calculate the survival rate.

[0022] Preparation of artificially simulated gastric juice: measure 16.4 ml of 9.5%-10.5% concentrated hydrochloric acid, add distilled water to 1000 ml, make basic artificial gastric juice, adjust pH value to 2.0, 3.0, 4.0 with hydrochloric acid or sodium hydroxide, take 10 mL (9 mL) each , divided into test tubes, steam sterilized at 100°C for 15 minutes, under sterile conditions, add 0.100g of pepsin to every 10mL of liquid.

[002...

Embodiment 2

[0033] 1) Cultivation of Bacillus licheniformis, primary culture: Inoculate the strains on the BPY plate medium, cultivate for 24 hours at 30°C, rejuvenate the Bacillus licheniformis, and form a single colony, pick a single colony and inoculate it on the slant medium, Cultivate at 30°C for 6 hours; Secondary cultivation: Inoculate Bacillus licheniformis on the first-grade BPY slant medium, and cultivate it at 30°C for about 16 hours to make it in the middle and late logarithmic period, and obtain first-grade seeds; Third-grade liquid cultivation: First-grade The first-grade seeds are made of bacterial suspension with sterile water, inoculated into BPY seed medium, the temperature is 30°C, the rotation speed is 200rpm, the tank pressure is 0.05Mpa, the ventilation ratio: 1:0.6, and cultivated for 14h to obtain the second-grade seed liquid and the seed liquid ; 2) liquid fermentation culture of Bacillus licheniformis, the inoculum size of the seed liquid is 3%, and the fermentati...

Embodiment 3

[0035] 1) Cultivation of Bacillus licheniformis, primary culture: inoculate the strains on BPY plate medium, culture at 37°C for 12 hours, rejuvenate Bacillus licheniformis and form a single colony, pick a single colony and inoculate it on the slant medium , cultured at 37°C for 24 hours; secondary culture: inoculate Bacillus licheniformis on the first-grade BPY slant medium, and cultivate it at 37°C for 12 hours to make it in the late logarithmic period, and obtain first-grade seeds; third-grade liquid culture: a The first-grade seeds are made of bacterial suspension with sterile water, inoculated into the BPY seed medium, the temperature is 37°C, the rotation speed is 250rpm, the tank pressure is 0.05Mpa, the ventilation ratio: 1:0.8, and cultivated for 10h to obtain the second-grade seed liquid; 2) For the liquid fermentation culture of Bacillus licheniformis, the inoculation amount of the seed liquid is 10%, and the fermentation is carried out until the number of bacteria r...

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Abstract

The invention relates to a preparation technology of a bacillus feed additive agent, belonging to the field of feed additive agent. The preparation technology comprises the following steps: 1) culturing bacillus to obtain a seed liquid; 2) carrying out liquid fermentation culture of bacillus with the inoculation amount of the seed liquid is 3-10% and until the microbial content reaches 1-2*10<10>cfu/ml; 3) collecting thalli by centrifugation; 4) freeze-drying thalli: adding a freeze-drying protectant in the collected thalli, putting the mixture in a freeze dryer for vacuum freeze-drying to obtain freeze-dried bacterial powder; and 5) adding auxiliary materials in the freeze-dried bacterial powder to obtain the bacillus feed additive agent. The feed additive agent obtained by the preparation technology has good probiotic functions of stress resistance and preventing pathogenic bacteria such as gastric acid resistance, cholate resistance, high temperature resistance, antibiotic tolerance and the like, can be used in combination with antibiotics. The prepared feed additive has high viable bacteria content, long -term storage, and obvious and stable application effects.

Description

technical field [0001] The invention relates to a preparation process of a bacillus feed additive and belongs to the field of feed additives. Background technique [0002] As a substitute for antibiotics, probiotics are attracting more and more attention from the feed industry due to their unique function of regulating intestinal flora. Currently, there are probiotics products on the market. However, the following major quality problems exist in the probiotics on the market: (1) low live bacteria content; (2) high moisture content, which is one of the important factors affecting the quality of probiotics; (3) Not resistant to antibiotics; (4) Unstable to gastric acid and bile salts, so that only a few strains are still active after entering the intestinal tract, and the number of viable bacteria required for functioning cannot be reached. Due to these quality problems of probiotics, the application effect on animals is affected, and the wide application of probiotics is lim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/17A23K20/195
Inventor 张玳华彭艺赵爱平贠桂玲
Owner BEIJING DABEINONG TECH GRP CO LTD
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