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Enterococcus faecium and the compound microecological preparation prepared therefrom

A technology of compound microecology and Enterococcus faecium, applied in microorganisms, microorganisms, microorganism-based methods, etc., can solve the problems of low survival rate, high cost, and aggravated bacterial drug resistance drug residues, so as to improve the survival rate, Good heat resistance, significant probiotic and stress resistance effects

Active Publication Date: 2020-02-14
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the survival rate of Lactobacillus after freeze-drying is not high, and it is one of the costly strains in the probiotics products for animals.
[0006] In addition, the continuous feeding of antibiotics has led to the increasing resistance of bacteria and drug residues in livestock products.

Method used

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  • Enterococcus faecium and the compound microecological preparation prepared therefrom
  • Enterococcus faecium and the compound microecological preparation prepared therefrom
  • Enterococcus faecium and the compound microecological preparation prepared therefrom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The present embodiment provides a new Enterococcus faecium, whose isolation, screening and identification are as follows:

[0029] Under aseptic conditions as far as possible, 1 g of the intestinal content of the rectum of healthy animals was collected with a sterile spoon, and placed in 30 mL of MRS medium (formulated peptone 10.0 g / L, beef powder 8.0 g / L, yeast powder 4.0 g / L) , Glucose 20.0g / L, K 2 HPO 4 2.0g / L, diamine hydrogen citrate 2.0g / L, sodium acetate 5.0g / L, MgSO 4 0.2g / L, MnSO 4 0.04g / L, Tween-801.0g / L. ) in a 100mL Erlenmeyer flask, enriched for 8 hours, then drew 1mL and 9mL sterilized saline to prepare a 1:10 dilution (that is, to prepare 10 -1 diluent), vibrate for 3-5min, accurately draw 1mL of the diluent into a 9mL sterilized saline test tube with a micropipette, vibrate with a vortex oscillator for 1-2min, and prepare 10 -2 diluent, followed by 10 -3 -10 -6 Dilution, select 10 -3 -10 -6 For three dilutions, draw 200 μL and spread them on ...

Embodiment 2

[0040] This example provides the performance testing of the probiotic and stress resistance of Enterococcus faecium E.F-2 isolated and identified in the above examples, wherein the test results of its biological characteristics of heat resistance, gastric acid resistance, and bile salt resistance As shown in table 2:

[0041] Table 2 Test results of Enterococcus faecium heat resistance, gastric acid resistance and bile salt resistance

[0042] Viable count Survival rate (according to 100%) normal saline 7.8×10 9 cfu

[0043] From the test results in Table 2, it can be seen that the survival rate of this strain of Enterococcus faecium and other existing Enterococcus faecium (NFER-5) after heat treatment at 60°C for 10 minutes is only 1.68×10 9 cfu / 9.40×10 9 cfu=1.24%, the survival rate of Enterococcus faecium isolated by Zhang Honggang and Li Li was between 1.2% and 79.1% under the condition of pH 2.0 for 2 hours, and the range of bile salt tolerance of ...

Embodiment 3

[0045] This embodiment provides the preparation process of the composite microecological preparation prepared from the Enterococcus faecium E.F-2 isolated and identified in the above embodiment as the main raw material, as follows:

[0046] Get a predetermined amount of Enterococcus faecium E.F-2 in high-efficiency fermentation medium (corn flour: 38.54g / L, urea: 4.57g / L, crystalline sodium acetate: 8.51g / L, dipotassium hydrogen phosphate: 3.0g / L L, anhydrous magnesium sulfate: 0.2g / L, manganese sulfate: 0.06g / L, diammonium hydrogen citrate: 2.0g / L, Tween-80: 1.0g / L) to carry out fermentation, after fermentation, rotate at a speed of 10000r Centrifuge under the condition of / min to obtain the bacteria sludge; add liquid culture medium to the bacteria sludge for resuspension, the volume ratio of the liquid culture medium and the original fermentation amount is 1:50-1:200, and then resuspend The protective agent was added to the liquid medium for thorough mixing, the mass ratio ...

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Abstract

The invention provides an Enterococcus faecium and a compound microecological preparation prepared therefrom, belonging to the technical field of lactic acid bacteria cultivation, capable of screening Enterococcus faecium strains with significant probiotic and stress resistance as feed additives, which can effectively replace some antibiotics. At the same time, it can be compounded with Lactobacillus to make a compound microecological preparation with high survival rate and long shelf life. The Enterococcus faecium provided by the technical solution is E.F‑2, and the preservation number is CCTCC No: M 2018314. The enterococcus faecium provided by the invention can be used as a safe and high-efficiency new feed additive to partially replace antibiotics, thereby meeting the requirements of the modern feed industry and animal husbandry.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria cultivation, and in particular relates to Enterococcus faecium and a composite microecological preparation prepared therefrom. Background technique [0002] Enterococcus faecium is a Gram-positive, facultative anaerobic bacterium belonging to the family Streptococcus and the genus Enterococcus. It has excellent biological characteristics and is an intestinal symbiotic bacterium that can form a dominant flora in the intestinal tract. Due to its fast growth rate, good adhesion, and the production of lactic acid and some antibacterial substances, it is found in the newborn 2 of many animals. -Have a dominant position within 3 days, thereby increasing the number of beneficial bacteria, inhibiting harmful bacteria, promoting intestinal health, regulating the micro-ecological balance of the intestinal tract and preventing diarrhea. It has a significant effect on improving growth performance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/46
Inventor 马丰英蒋贻海王艳玲王宏华崔栩孙亚磊刘元元魏波武利利
Owner QINGDAO VLAND BIOTECH INC
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