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Pediococcus acidilactici separated from Tibetan kefir and application of pediococcus acidilactici in prevention and treatment of rotavirus infection

A technology of Pediococcus lactis and self-contained spirit mushrooms, applied in the field of biomedicine, can solve problems such as death, affecting growth and development and feed conversion rate, and damaging the economic benefits of pig raising

Active Publication Date: 2022-02-08
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pigs of all ages can be infected. Among them, suckling piglets and piglets before and after weaning are more common. They mainly show a series of serious gastrointestinal symptoms, which affect growth and feed conversion rate to a certain extent. Cause death and damage the economic benefits of pig farming

Method used

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  • Pediococcus acidilactici separated from Tibetan kefir and application of pediococcus acidilactici in prevention and treatment of rotavirus infection
  • Pediococcus acidilactici separated from Tibetan kefir and application of pediococcus acidilactici in prevention and treatment of rotavirus infection
  • Pediococcus acidilactici separated from Tibetan kefir and application of pediococcus acidilactici in prevention and treatment of rotavirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Screening of Lactic Acid Bacteria in Zangling Mushroom

[0057] 1. Materials

[0058] Tibetan Linggu (stored at -80°C), phosphate buffer, MRS agar plate, anaerobic tank, 1.5ml centrifuge tube, biochemical fermentation tube, PCR enzyme, primers, deionized water

[0059]MRS medium: glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, magnesium sulfate 0.5g / L, manganese sulfate 0.2g / L, ammonium citrate 2g / L, sodium acetate 5g / L, Tween-80 1ml / L, pH value 6.0±0.05, sterilized at 121°C for 20min. Solid medium needs to add 1.2% agar.

[0060] Lactic acid bacteria identification medium: water 100ml, beef extract 1g, peptone 1g, yeast extract 1g, tomato juice 20g, glucose 1g, Tween 0.05ml, calcium carbonate 1.7g, bromocresol green 0.01g, agar 1-2g, 121℃ Sterilize for 20 minutes.

[0061] Reagent preparation

[0062] TAE: 50* Preparation of TAE: Tris: 242g, Na2EDTA·2H2O: 37.2g, add 800ml of deionized water into the beaker, and stir well. Then 5...

Embodiment 2

[0151] Example 2 Study on the inhibitory effect on rotavirus

[0152] 1. Materials

[0153] 6-well cell culture plate, 1640 cell culture medium, double antibody, fetal bovine serum, porcine intestinal epithelial cells (IPEC-J2 cells), ribavirin, gentamicin, PBS, plasmid extraction kit, PCR enzyme, primers , deionized water, gavage needle, ELISA kit (IFN-β, IL-6, TNF-α, SIgA), fluorescent quantitative enzyme, internal reference β-actin, porcine rotavirus, 0.22 μm filter, reverse transcription reagent box

[0154] Experimental animals: 3-4 week-old female BALB / c mice (purchased from Beijing Huafukang Biotechnology Co., Ltd.). and housed in SPF animal housing.

[0155] MRS medium: glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, magnesium sulfate 0.5g / L, manganese sulfate 0.2g / L, ammonium citrate 2g / L, sodium acetate 5g / L, Tween-80 1ml / L, pH value 6.0±0.05, sterilized at 121°C for 20min. Solid medium needs to add 1.2% agar.

[0156] 10% 1640 cell cul...

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Abstract

The invention provides pediococcus acidilactici separated from Tibetan kefir and application of the pediococcus acidilactici in prevention and treatment of rotavirus infection, the pediococcus acidilactici separated from the Tibetan kefir is named as pediococcus acidilactici Wang, the pediococcus acidilactici is preserved in the China Center for Type Culture Collection on October 28, 2021, and the preservation number is CCTCC NO: M 20211331. The pediococcus acidilactici is resistant to acid and cholate, has a good inhibiting effect on most pathogenic bacteria, is sensitive to antibiotics, and shows an excellent rotavirus resisting effect in in-vivo and in-vitro experiments.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of Pediococcus lactis isolated from Zangling mushroom and its prevention and treatment of rotavirus infection. Background technique [0002] The information disclosed in the Background of the Invention is intended to enhance the understanding of the general background of the invention, and the disclosure should not necessarily be construed as an acknowledgment or in any form to imply that the information has become the prior art that is already known to those skilled in the art. [0003] Rotavirus is one of the main pathogens that cause diarrhea in infants and young animals. About 215,000 infants and young children die of RV infection in the world every year, most of which occur in developing countries. At present, there is no anti-RV specific drug. Vaccination of live attenuated vaccine is the most economical and effective means to prevent and control RV diar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/744A61P31/14A61P37/04A23K10/18C12R1/01
CPCC12N1/20A61K35/744A61P31/14A61P37/04A23K10/18
Inventor 王春凤杨桂连牛天明杨文涛石春卫叶丽萍张博文江雨鑫李楠包军付王红谷巍单宝龙
Owner JILIN AGRICULTURAL UNIV
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