Method for preparing butyric acid bacteria powder by microencapsulated propagation culture and application

A proliferation culture and microencapsulation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems that there are no patent reports on butyric acid bacteria, and achieve the prevention of intestinal diseases, low production cost, and tolerance Good effect of bile salt

Active Publication Date: 2011-09-28
江苏省苏微微生物研究有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention relates to microencapsulation technology for embedding butyric acid bacteria and its method for high-density cultivation of butyric acid bacteria. At present, there is no patent report about butyric acid bacteria

Method used

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  • Method for preparing butyric acid bacteria powder by microencapsulated propagation culture and application
  • Method for preparing butyric acid bacteria powder by microencapsulated propagation culture and application
  • Method for preparing butyric acid bacteria powder by microencapsulated propagation culture and application

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: the preparation method of butyric acid bacteria microcapsule particles (1)

[0033] (1) Strain activation and expanded culture: Aseptically open the freeze-dried strain CGMCC No.1647, insert it into a test tube containing the activation medium, carry out static anaerobic culture, and cultivate it at 37°C for 18 hours, and then use a volume ratio of 2 % of the inoculum was transferred to fresh activation medium and cultured at 37°C for 24h. Microscopic examination shows that when more than 90% of the bacteria form spores, they are mature and activated 2 to 3 times. The seed bacteria suspension was transferred into the Erlenmeyer flask containing the fermentation medium with the inoculum amount of 1%-10% (V / V), and the liquid volume of the Erlenmeyer flask was 90% (V / V), and it was placed in a constant temperature incubator at 37°C Cultivate for 22-24 hours.

[0034] Butyric acid bacteria seed activation medium consists of the following raw materials and w...

Embodiment 2

[0041] Embodiment 2: the preparation method of butyric acid bacteria microcapsule particles (two)

[0042] (1) Strain activation and expanded cultivation are the same as in Example 1.

[0043] (2) Microencapsulation embedding: prepare sodium alginate with a weight percent concentration of 4.0%, sodium lactate at 0.3%, gelatin at 0%, and soluble starch at 0.5%, and mix them to obtain a microcapsule wall material. The solvent used is for deionized water. A calcium chloride deionized aqueous solution with a concentration of 3.0% by weight was prepared and sterilized for use.

[0044] According to the volume ratio of bacterial liquid: wall material = 1:3.5, the mixed glue of wall material and bacterial liquid is prepared. After fully stirring, the mixed liquid is sprayed to the sterilized concentration of magnetic stirring by using a high-voltage electrostatic capsule forming device. 200 microcapsules of 2-3 mm can be prepared per ml of bacterial liquid in 3.0% calcium chloride ...

Embodiment 3

[0046] Embodiment 3: the preparation method of butyric acid bacteria microcapsule particles (three)

[0047] (1) Strain activation and expanded cultivation are the same as in Example 1.

[0048] (2) Microencapsulation embedding: prepare sodium alginate with a concentration of 2.0% by weight, sodium lactate at 0.5%, gelatin at 1.0%, and bentonite at 0.5%, and mix them to obtain wall materials for microcapsules. The solvent used is Deionized water. Calcium chloride deionized aqueous solution with a concentration of 2.5% by weight was prepared and sterilized for use.

[0049] According to the volume ratio of bacterial liquid: wall material = 1:5, prepare the mixed glue of wall material and bacterial liquid. After fully stirring, use high-voltage electrostatic capsule forming device to spray the mixed liquid to the sterilized concentration of magnetic stirring. In 2.5% calcium chloride deionized aqueous solution, form microcapsules of 2-3 mm, stand and solidify for 22-24 hours, ...

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Abstract

The invention discloses a method for preparing butyric acid bacteria powder by microencapsulated propagation culture and application, which belong to the technical field of microbial agents. In the invention, an initial strain is clostridium butyricunm JSIM-MCB20040312 with the preservation number of CGMCC (China General Microbiological Culture Collection) No.1647; the butyric acid bacteria powder is prepared by the steps of activation of butyric acid bacteria seeds, microencapsulated embedding of the butyric acid bacteria seeds, viable counting and preparation of butyric acid bacteria powder, wherein the concentration of the obtained bacteria powder is not less than 1.0*1011 / g. The viable organism propagation capacity of the butyric acid bacteria subjected to microencapsulated embedding is enhanced; a strict anaerobic environment created by liquid sealing of nitrogen gas or carbon diode and other inert gases or paraffin is avoided; a high-concentration bacteroid can be obtained without introducing air into a general fermentation tank for culturing; the production cost is low and the requirement for fed microbial additives on viable count can be met; the butyric acid bacteria powder also has the characteristics of heat resistance, acid resistance and favorable entericsolubility and is suitably used as animal intestine probiotics; and intestinal diseases of livestock and poultry farming animals such as chicken, pigs and the like are effectively prevented and the growth of the bred animals is promoted.

Description

technical field [0001] The invention discloses a method for preparing butyric acid bacteria powder by microencapsulation and multiplication culture and its application, and relates to a method for high-density culture of heat-resistant butyric acid bacteria and its application. The invention belongs to the technical field of microbial preparations. Background technique [0002] Clostridium butyricum, also known as butyric acid bacteria, Clostridium butyricum, ( Clostridium butyricum ), hereinafter referred to as butyric acid bacteria, is an anaerobic beneficial bacterium that exists in the intestines of humans and livestock. The spores of Clostridium butyricum are resistant to heat, acid and bile, and are ideal strains for feeding probiotics. Butyric acid bacteria can produce butyric acid, lactic acid, etc., promote the proliferation of intestinal beneficial bacteria such as bifidobacteria and lactic acid bacteria, and inhibit the growth of staphylococcus, Escherichia coli,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C12N11/10C12N11/12A23K1/16C12R1/145A23K10/18
Inventor 孙梅匡群陈秋红何义进谢骏施大林张维娜史济筠邹益东
Owner 江苏省苏微微生物研究有限公司
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