A kind of Lactobacillus reuteri, microbial agent and food product

A technology of Lactobacillus reuteri and microbial agent, applied in the field of microbiology, can solve the problems of few related researches, achieve the effects of prolonging the survival period, reducing the incidence of tumors, high research value and application value

Active Publication Date: 2021-11-02
深圳市沁帆科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there are not many studies on the relationship between endogenous commensal bacteria and tumor cells in mammalian organs

Method used

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  • A kind of Lactobacillus reuteri, microbial agent and food product
  • A kind of Lactobacillus reuteri, microbial agent and food product
  • A kind of Lactobacillus reuteri, microbial agent and food product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation and identification of Lactobacillus reuteri CCTCC M20191116

[0027] Blood was collected from 8-week-old female healthy BALB / C mice, soaked and sterilized with neogilt, and then dissected. The liver and kidneys were ground to a slurry with a tissue grinder, diluted with normal saline, and spread on Put 1% vancomycin on the MRS plate, put it in an anaerobic constant temperature incubator at 37°C and cultivate it for 48 hours, pick a single colony after the colony grows, separate it by streaking on the plate, carry out repeated purification and culture, and use Gram staining Gram-positive bacteria were selected by observing the colony morphology. After the isolated strains were cultured in liquid, they were cultured on a plate, and a single colony was picked with an inoculation loop as a template for colony PCR. Send positive PCR products to the company for sequencing.

[0028] The 16S rRNA base sequence analysis and sequencing base sequence of the ...

Embodiment 2

[0030] Example 2: Acid resistance and bile salt resistance of L.reuteri CCTCC M20191116 and 10% skim milk mixture

[0031] During the digestion process of probiotics in the stomach and intestines, in addition to acid stress and bile salt stress, there are various digestive enzymes that may cause damage to the bacteria or lead to their death, resulting in the activity of finally reaching the key role site of the intestinal tract. The number of bacteria was greatly reduced. Experimental results such as figure 1 As shown, the survival rate of PBS mixture is only 0.1%, while the bacterial suspension of 10% skim milk is 10% of the total number of initial bacteria after the simulated transfer. It can greatly protect the vitality of Lactobacillus, help Lactobacillus reach the intestinal tract, and ensure its corresponding function. The ability of Lactobacillus reuteri CCTCCM20191116 mixed with 10% skim milk to resist acid and bile salts is 100 times that of the control group. Mixin...

Embodiment 3

[0032] Example 3: Changes in the body weight of mice in the prevention group administered with Lactobacillus reuteri CCTCC M20191116

[0033] BALB / C mice were modeled with melanoma cells by subcutaneous injection, and the occurrence of tumors in the mice could be observed in about 15 days, and the tumors continued to grow, and the weight gradually increased, and the body weight increased rapidly in the later stage. figure 2 shown. The mice in the model group began to die on the 36th day, and 100 μL of 10 8 CFU / mL Lactobacillus reuteri CCTCC M20191116 non-fat milk suspension (10% skim milk) gavage group and the control group also gavage 100 μL 10% skim milk every three days did not die.

[0034] like figure 2 It has been shown that intragastric administration of Lactobacillus reuteri CCTCC M20191116 can alleviate the weight gain of mice caused by tumors, and alleviate the burden of tumor bearing on the host to a certain extent.

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PUM

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Abstract

The invention discloses a Lactobacillus reuteri (Lactobacillus reuteri CCTCC M20191116), which can significantly prevent the occurrence of tumors and significantly prolong the survival of tumor-bearing mice by detecting its function of antagonizing tumor cells through cell experiments and animal models time. Tumor-bearing mice fed with L. reuteri CCTCC M20191116 can maintain normal alanine aminotransferase levels, indicating that L. reuteri CCTCC M20191116 can prevent melanoma cells from injuring the liver. The invention belongs to the technical field of microorganisms. The provided L. reuteri CCTCC M20191116 can stimulate the body to produce anti-cancer cytokines, inhibit the migration and colonization of tumor cells, protect internal organs, and have the functions of preventing tumor occurrence and prolonging survival time.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a new strain of Lactobacillus reuteri and its probiotic function, especially the function for antagonizing tumors. Background technique [0002] People from all over the world have a tradition of eating probiotic fermented foods since ancient times. Metchnikoff et al. found that the longevity of Bulgarian farmers is closely related to their preference for yogurt containing Lactobacillus. Lactobacillus is a probiotic in the general sense. Current research has found that there are more than 170 species of Lactobacillus, which can improve and maintain the body's normal immune system, produce antibacterial substances, enhance the mucosal barrier function, antagonize pathogenic bacteria, and fight tumors. With the continuous in-depth research, the interaction between tumors and probiotics has received extensive attention. Since 99% of microorganisms reside in the gastrointestinal tract...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P35/00A61P1/16A61P17/00C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61P35/00A61P1/16A61P17/00A23V2002/00C12R2001/225C12N1/205A23V2400/173A23V2200/308
Inventor 徐锋陈沁罗梦武晓丽
Owner 深圳市沁帆科技有限公司
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