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A kind of Enterococcus faecium r40 and its application in lowering cholesterol, producing exopolysaccharide and antioxidation

A technology of Enterococcus faecium and cholesterol reduction, applied in the field of microorganisms, can solve the problems of no cholesterol reduction, extracellular polysaccharide production and anti-oxidation, and achieve good bile salt resistance, good taste and strong tolerance

Active Publication Date: 2022-03-01
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current reports about lactic acid bacteria, it is mentioned that some Enterococcus faecium has the effect of lowering cholesterol, but there is no relevant report about the effect of lowering cholesterol, producing exopolysaccharide and anti-oxidation at the same time; Cholesterol, exopolysaccharide producing and antioxidant strains are of interest

Method used

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  • A kind of Enterococcus faecium r40 and its application in lowering cholesterol, producing exopolysaccharide and antioxidation
  • A kind of Enterococcus faecium r40 and its application in lowering cholesterol, producing exopolysaccharide and antioxidation
  • A kind of Enterococcus faecium r40 and its application in lowering cholesterol, producing exopolysaccharide and antioxidation

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Experimental program
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Effect test

Embodiment 1

[0015] The isolation, screening and identification of embodiment 1 Enterococcus faecium R40

[0016] 1. Isolation of Enterococcus faecium R40

[0017] Take samples (fish intestinal content, kimchi soup, gastrointestinal drugs) and dissolve them in 10mL sterile saline, then perform gradient dilution on them, select an appropriate dilution factor, and draw 0.1mL of the diluted solution and apply it on the solid MRS+ CaCO 3 Culture medium, 37 ℃, static culture 24h. Pick colonies with obvious transparent circles and Gram-positive staining on the culture medium, respectively streak and purify until the colony has a single shape, observe and record the basic morphological characteristics of the colonies, and pick the colonies in 30% glycerol tubes and place them in an ultra-low temperature refrigerator- Store at 70°C. Among them, the above-mentioned gastrointestinal medicines are "Mummy Love" Bacillus subtilis double live bacteria granule, golden bifido (bifidobacterium Lactobaci...

Embodiment 2

[0073] Example 2 Exopolysaccharide experiment of Enterococcus faecium R40

[0074] 1. Activate Enterococcus faecium R40 and inoculate it into the liquid exopolysaccharide MRS liquid screening medium, and culture it statically at 37°C for 48 hours. Take 10 mL of the culture solution in a test tube and add 1 / 3 volume of Sevag reagent (chloroform: n-butanol =4:1), after shaking for 30min, centrifuge at 4000r / min for 15min, collect the upper aqueous phase solution, repeat the above operation until there is no protein layer between the two phases. Then add 3 times the volume of frozen absolute ethanol, and place it in a refrigerator at 4°C. Take it out on the second day, and finally the polysaccharide can be observed as a milky white flocculent precipitate, then centrifuge at 4°C and 6000g for 30 minutes, dissolve the precipitate with 10mL distilled water, and obtain the exopolysaccharide extract, which is freeze-dried from the crude exopolysaccharide extract Extracellular crude p...

Embodiment 3

[0082] Antioxidant experiment of embodiment 3 Enterococcus faecium R40

[0083] 1. Scavenging effect on DPPH free radicals

[0084] Add 100 μL of 1 g / L extracellular crude polysaccharide aqueous solution to 100 μL DPPH radical absolute ethanol solution (0.2 mmol / L), stir vigorously, and incubate at room temperature in the dark for 30 min; use deionized water and DPPH solution as controls, Use the sample and absolute ethanol as a blank; measure the absorbance at 517nm, and repeat at least three times for each group.

[0085] DPPH clearance rate / %=[1-(A 样品 -A 空白 ) / A 对照 ]×100

[0086] Draw a standard curve with Trolox as a standard, and obtain the standard curve regression equation as Y=0.5673X+9.0774, R 2 = 0.9946. The EPS content of the sample extract was calculated according to the standard curve, in terms of Trolox equivalent per g of the extract (vitamin E mg / g).

[0087] 2. Scavenging effect on ABTS free radicals

[0088] Accurately prepare 7.0mmol / L ABTS solution a...

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Abstract

The invention provides an Enterococcus faecium R40 and its application in lowering cholesterol, producing exopolysaccharide and anti-oxidation. The Enterococcus faecium R40 has the functions of lowering cholesterol, producing extracellular polysaccharides and anti-oxidation, and is more suitable for growth under acidic conditions. It is more tolerant to gastrointestinal conditions and has relatively good bile salt tolerance. Live bacteria or metabolites of Enterococcus faecium R40 can be added to food to lower cholesterol, resist oxidation, and aid digestion in the human body. The present invention also includes the application of Enterococcus faecium R40 in yoghurt and the yoghurt containing Enterococcus faecium R40. The yoghurt is better than commercial starter fermented yoghurt in every index.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an Enterococcus faecium R40 and its application in lowering cholesterol, producing exopolysaccharide and antioxidation. Background technique [0002] With people's strong awareness and demand for health, probiotics, prebiotics, and functional foods have gradually become the focus of people's daily diet. In the 2020 International Symposium on Probiotics and Health, relevant experts organized by the Chinese Society of Food Science and Technology reached a consensus on the basic characteristics of probiotics: the strains are in a viable state, the number of strains is sufficient, and they have health benefits. In recent years, the main focus of the research on probiotics is its medical aspects—such as the improvement of intestinal function, the treatment of respiratory diseases, etc.; the aspect of animal husbandry—such as the use of probiotics to replace antibiotics or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23C9/12C12R1/01
CPCC12N1/20A23L33/135A23C9/1203A23V2002/00C12R2001/46C12N1/205A23V2200/3262A23V2200/30
Inventor 焦士蓉包善思王文杰孙博瑞姚瑶杨浩
Owner XIHUA UNIV
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