Probiotic preparation containing Lactobacillus plantarum and Bacillus licheniformis and preparation method thereof
A technology of Bacillus licheniformis and Lactobacillus plantarum, which is applied in the field of probiotic fermentation, can solve the problems of large environmental pollution, difficult post-processing, cumbersome operation of machinery and equipment, etc., and achieves low capital investment, low production cost, and good application and development prospects Effect
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Embodiment 1
[0030] Example 1: Mixed solid-state fermentation of probiotic preparations
[0031] (1) Preparation of bacterial seed solution
[0032]Bacillus licheniformis BL-09 was inoculated into the liquid seed medium from a frozen glycerol tube, placed on a shaker with a rotation speed of 150-180r / min and a temperature of 30-37°C, and cultivated for 20-24h. Lactobacillus plantarum HM-10 is inoculated into the liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature is 30-37°C, and the cultivation time is 20-24h. The liquid seed medium is (g / L): peptone 10-20, glucose 10-20, the rest is water, pH 7.0-7.2.
[0033] (2) Solid state fermentation
[0034] First inoculate Lactobacillus plantarum HM-10 in the solid-state fermentation medium with 1-5% inoculation amount (v / w), control the temperature at 28-32°C, and relative humidity at 50%-60%; 8% of the inoculum was inserted into Bacillus licheniformis BL-09 for mixed solid-state fe...
Embodiment 2
[0040] Embodiment 2: the impact of Lactobacillus plantarum HM-10 inoculum size on the number of viable bacteria
[0041] Lactobacillus plantarum HM-10 was inoculated into the liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature was 37°C, and the cultivation time was 20h. The composition of the liquid seed culture medium is (g / L): 10 peptone, 20 glucose, the rest is water, and the pH is 7.0-7.2.
[0042] The solid-state fermentation medium is composed of soybean meal, bran and corn flour, and the mass ratio of the soybean meal, bran and corn flour is: soybean meal, bran and corn flour=10:10:0.5. The moisture content was controlled at 70%, and sterilized at 115°C for 20 minutes.
[0043] The solid medium was inoculated with 1% (v / w), 3% (v / w), and 5% (v / w) Lactobacillus plantarum HM-10, and the Lactobacillus plantarum in the fermentation product was measured after 48 hours of single-bacteria fermentation. The number...
Embodiment 3
[0045] Embodiment 3: the influence of bacillus licheniformis BL-09 inoculum amount on viable count
[0046] Bacillus licheniformis BL-09 was inoculated into the liquid seed medium from a frozen glycerol tube, placed on a shaker with a rotation speed of 180 r / min and a temperature of 37°C, and cultivated for 20 hours. Lactobacillus plantarum HM-10 was inoculated into the liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature was 37°C, and the cultivation time was 20h. The composition of the liquid seed culture medium is (g / L): 10 peptone, 20 glucose, the rest is water, and the pH is 7.0-7.2.
[0047] The solid-state fermentation medium is composed of soybean meal, bran and corn flour, and the mass ratio of the soybean meal, bran and corn flour is: soybean meal, bran and corn flour=10:10:0.5. The moisture content is controlled at 60%, and it is sterilized at 115°C for 20 minutes.
[0048] Inoculate 1% (v / w) Lactobacil...
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