Probiotic preparation containing Lactobacillus plantarum and Bacillus licheniformis and preparation method thereof

A technology of Bacillus licheniformis and Lactobacillus plantarum, which is applied in the field of probiotic fermentation, can solve the problems of large environmental pollution, difficult post-processing, cumbersome operation of machinery and equipment, etc., and achieves low capital investment, low production cost, and good application and development prospects Effect

Active Publication Date: 2016-05-25
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment

Method used

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  • Probiotic preparation containing Lactobacillus plantarum and Bacillus licheniformis and preparation method thereof
  • Probiotic preparation containing Lactobacillus plantarum and Bacillus licheniformis and preparation method thereof
  • Probiotic preparation containing Lactobacillus plantarum and Bacillus licheniformis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Mixed solid-state fermentation of probiotic preparations

[0031] (1) Preparation of bacterial seed solution

[0032]Bacillus licheniformis BL-09 was inoculated into the liquid seed medium from a frozen glycerol tube, placed on a shaker with a rotation speed of 150-180r / min and a temperature of 30-37°C, and cultivated for 20-24h. Lactobacillus plantarum HM-10 is inoculated into the liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature is 30-37°C, and the cultivation time is 20-24h. The liquid seed medium is (g / L): peptone 10-20, glucose 10-20, the rest is water, pH 7.0-7.2.

[0033] (2) Solid state fermentation

[0034] First inoculate Lactobacillus plantarum HM-10 in the solid-state fermentation medium with 1-5% inoculation amount (v / w), control the temperature at 28-32°C, and relative humidity at 50%-60%; 8% of the inoculum was inserted into Bacillus licheniformis BL-09 for mixed solid-state fe...

Embodiment 2

[0040] Embodiment 2: the impact of Lactobacillus plantarum HM-10 inoculum size on the number of viable bacteria

[0041] Lactobacillus plantarum HM-10 was inoculated into the liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature was 37°C, and the cultivation time was 20h. The composition of the liquid seed culture medium is (g / L): 10 peptone, 20 glucose, the rest is water, and the pH is 7.0-7.2.

[0042] The solid-state fermentation medium is composed of soybean meal, bran and corn flour, and the mass ratio of the soybean meal, bran and corn flour is: soybean meal, bran and corn flour=10:10:0.5. The moisture content was controlled at 70%, and sterilized at 115°C for 20 minutes.

[0043] The solid medium was inoculated with 1% (v / w), 3% (v / w), and 5% (v / w) Lactobacillus plantarum HM-10, and the Lactobacillus plantarum in the fermentation product was measured after 48 hours of single-bacteria fermentation. The number...

Embodiment 3

[0045] Embodiment 3: the influence of bacillus licheniformis BL-09 inoculum amount on viable count

[0046] Bacillus licheniformis BL-09 was inoculated into the liquid seed medium from a frozen glycerol tube, placed on a shaker with a rotation speed of 180 r / min and a temperature of 37°C, and cultivated for 20 hours. Lactobacillus plantarum HM-10 was inoculated into the liquid seed medium, placed in a constant temperature incubator for static cultivation, the cultivation temperature was 37°C, and the cultivation time was 20h. The composition of the liquid seed culture medium is (g / L): 10 peptone, 20 glucose, the rest is water, and the pH is 7.0-7.2.

[0047] The solid-state fermentation medium is composed of soybean meal, bran and corn flour, and the mass ratio of the soybean meal, bran and corn flour is: soybean meal, bran and corn flour=10:10:0.5. The moisture content is controlled at 60%, and it is sterilized at 115°C for 20 minutes.

[0048] Inoculate 1% (v / w) Lactobacil...

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Abstract

The invention relates to a probiotic preparation containing Lactobacillus plantarum and Bacillus licheniformis and a preparation method thereof, belonging to the technical field of fermentation of probiotics. The invention particularly relates to a mixed solid state fermentation technique which uses bean pulp, bran and corn flour as matrixes and the probiotics Lactobacillus plantarum HM-10 and Bacillus licheniformis BL-09 as strains. The technique can be widely used in the aspects of animal microecology and the like. The method can be used for producing fermented feeds with special acid aroma; the viable count of the fermented product is high; and the degradation rate of the bean pulp protein is high. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

Description

technical field [0001] The invention belongs to the technical field of probiotic fermentation, and in particular relates to a kind of probiotics Lactobacillus plantarum HM-10 (Lactobacillus plantarum HM-10) and Bacillus licheniformis BL-09 (Bacillus licheniformis BL-09) with soybean meal, bran and corn flour as substrates It is a mixed solid-state fermentation technology of strains, which can be widely used in animal microecology and other aspects. Background technique [0002] At present, probiotic products mostly use probiotics to directly ferment with liquid milk and soy milk to obtain functional yogurt or soy milk, and the rest are fermented in liquid state to directly collect probiotic cells and make probiotic powder, tablets or capsules. The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment. Therefore, this application uses...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/10C12R1/25
Inventor 包维臣高鹏飞姚国强
Owner INNER MONGOLIA SCI PLUS BIOTECH
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