Bacillus licheniformis probiotic preparation and its preparation

A technology of Bacillus licheniformis and probiotic preparations, which is applied in the field of feed microorganisms, can solve the problems of cumbersome operation of machinery and equipment, large environmental pollution, and difficult post-processing, and achieve the effects of facilitating gastrointestinal digestion and absorption, simple equipment structure, and low cost

Active Publication Date: 2016-09-21
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment.

Method used

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  • Bacillus licheniformis probiotic preparation and its preparation
  • Bacillus licheniformis probiotic preparation and its preparation
  • Bacillus licheniformis probiotic preparation and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Combination figure 1 The effect of the inoculum amount of Bacillus licheniformis BL-09 on the number of viable bacteria is explained

[0044] Such as figure 1 As shown, the inoculation amount of Bacillus licheniformis BL-09 was 2.0% (v / w), the culture temperature was 37±1°C, and the culture time was 24 hours. 10 cfu / g.

[0045] The inoculum amount of Bacillus licheniformis BL-09 was 5.0% (v / w), the culture temperature was 37±1°C, and the culture time was 24 hours. The number of live bacteria of Bacillus licheniformis BL-09 was 1.55×10 10 cfu / g.

[0046] The inoculum amount of Bacillus licheniformis BL-09 was 8.0% (v / w), the culture temperature was 37±1°C, and the culture time was 24 hours. The number of live bacteria of Bacillus licheniformis BL-09 was 1.31×10 10 cfu / g.

[0047] Results: The optimal inoculation amount of Bacillus licheniformis BL-09 was 5.0% (v / w) during fermentation.

Embodiment 2

[0048] Example 2: Combination figure 2 Explain the effect of solid-state fermentation time on the number of viable bacteria

[0049] Bacterial growth has gone through lag phase, logarithmic growth phase, equilibrium phase and recession phase respectively. In different phases, the growth conditions are different, and the live bacteria counts are taken respectively.

[0050] The solid-state fermentation medium is: 29% soybean meal, 29% bran, 2% corn flour, moisture content 40%, autoclaved at 115°C for 25min.

[0051] Such as figure 2 As shown, the inoculum amount of Bacillus licheniformis BL-09 was 5.0% (v / w), the culture temperature was 37±1°C, and the culture time was 24 hours. 10 cfu / g.

[0052] The inoculation amount of Bacillus licheniformis BL-09 was 5.0% (v / w), the culture temperature was 37±1°C, and the culture time was 36 hours. The number of live bacteria of Bacillus licheniformis BL-09 was 1.36×10 10 cfu / g.

[0053] The inoculum amount of Bacillus licheniformis...

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Abstract

The invention discloses a bacillus licheniformis probiotic preparation and a preparation method thereof, and belongs to the microbial technical field of feeds. The invention relates to a solid state fermentation technology which takes soybean meal, bran and corn flour as substrates and adopts bacillus licheniformis BL-09 as a strain, and is widely applicable to such aspects as animal microecology. Specifically, the preparation method comprises bacillus licheniformis BL-09 seed culture and solid state fermentation culture, wherein a seed medium contains the following materials in percentage by mass: 1% of peptone, 2% of glucose and the balance being water to 100%, wherein pH value is 7.0-7.2; a solid state fermentation medium contains the following materials in percentage by mass: 29% of soybean meal, 29% of bran, 2% of corn flour and 40% of water. Through fermentation by the bacillus licheniformis BL-09, a degradation rate of nutrient substances is increased; acid resistance, heat resistance and cholate resistance are good, so that the preparation method is suitable for complex processing steps of the feed; a relatively high viable count and a high activity are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of feed microorganisms, and relates to a solid-state fermentation technology using soybean meal, bran and corn flour as substrates and probiotic Bacillus licheniformis BL-09 (Bacillus licheniformis BL-09) as strains, which can be widely used Animal Microecology etc. Background technique [0002] Probiotics have many benefits such as preventing and treating diarrhea, enhancing immunity, promoting digestive system health, and lowering serum cholesterol, so more and more people are paying attention to them. The research and application of probiotics are in a period of rapid development in China, but this rapid The development has not yet risen from active lactic acid bacteria to the level of probiotics. The wider application and acceptance is still limited to the direct fermentation of lactic acid bacteria with liquid milk and soy milk to obtain functional yogurt or soy milk. Collect probiotic bacteria and mak...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/10A23K10/12
Inventor 姚国强高鹏飞张善亭
Owner INNER MONGOLIA SCI PLUS BIOTECH
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