Natural astaxanthin-containing premix compound
A technology of natural astaxanthin and premix, which is applied in the field of premix containing natural astaxanthin, can solve the problems that premix animal feeding fails to achieve the expected effect, does not meet the needs of human beings for greenness and safety, etc. , to achieve the effect of promoting feed conversion efficiency, appropriate color, and reducing diseases
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Embodiment 1
[0019] Embodiment 1 prepares compound probiotics
[0020] 1. Preparation of Bacillus licheniformis CGMCC No.5094 bacterial powder
[0021] 1) Plate culture rejuvenation: Streak the Bacillus licheniformis species on the BPY plate medium with an inoculation loop, culture at 37°C for 24 hours, rejuvenate the Bacillus licheniformis and form a single colony, pick a single colony and inoculate it on the slant medium cultured at 37°C for 36h;
[0022] 2) Preparation of first-class seeds: transfer the Bacillus licheniformis cultured in step 1) to the eggplant bottle slant medium, and cultivate them at 37° C. for 12 hours to make them in the late logarithmic period to obtain first-class seeds; The slant medium is BPY solid medium;
[0023] 3) Preparation of secondary seeds: make bacterial suspension from the primary seeds prepared in step 2) with sterile water, and inoculate into 1.6M 3 2M of BPY seed medium 3 In the seed tank, the temperature is 37°C, the rotation speed is 200rpm,...
Embodiment 2
[0038] The preparation of embodiment 2 premix
[0039] 1) Preparation of compound probiotics
[0040] Take by weighing the Bacillus licheniformis CGMCC No.5094 bacterium powder prepared in Example 1, Enterococcus faecalis CGMCC No.5092 bacterium powder, Saccharomyces spp. : 6: 49 mixed to obtain a compound probiotics. Wherein the live bacteria content of the above three bacteria powders are respectively: Enterococcus faecalis 4×10 10 cfu / g, Bacillus licheniformis 3×10 10 cfu / g, Saccharomyces spp. 6×10 9 cfu / g.
[0041] 2) Preparation of premix
[0042] The premix formula per kilogram is as follows: broken wall Haematococcus pluvialls powder 40g (the astaxanthin content of the broken wall Haematococcus pluvialls residue after extracting astaxanthin is measured by colorimetry is 0.2%), soybean meal 200g, Calcium hydrogen phosphate 160g, stone powder 200g, salt 60g, niacin 0.75g, D-calcium pantothenate 0.15g, biotin 0.1g with a content of 2%, choline chloride with a content...
Embodiment 3
[0057] The preparation of embodiment 3 premix
[0058] 1) Preparation of composite microecological preparation: take the Bacillus licheniformis CGMCC No.5094 bacterial powder prepared in Example 1, Enterococcus faecalis CGMCC No.5092 bacterial powder, Saccharomyces spp. CGMCC No.2387 bacterial powder and glucose, The mixture is mixed at a weight ratio of 20:25:5:50 to obtain a compound microecological preparation. Wherein the live bacteria content of the above three bacteria powders are respectively: Enterococcus faecalis 3×10 10 cfu / g, Bacillus licheniformis 4×10 10 cfu / g, yeast 5 × 10 9 cfu / g.
[0059] 2) the preparation of premix of the present invention:
[0060] The premix formula per kilogram is as follows: broken wall Haematococcus pluvialls powder 30g (the astaxanthin content of the broken wall Haematococcus pluvialls residue after extracting astaxanthin is measured by colorimetry is 0.4%), soybean meal 300g, Calcium hydrogen phosphate 150g, stone powder 150g, sal...
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