Method for preparing oil cake protein feedstuff through low moisture solid state fermentation

A cake protein, solid-state fermentation technology, applied in animal feed, animal feed, applications, etc., can solve the problems of drying, crushing difficulties, agglomeration, etc., to reduce bacterial pollution, stable product quality, and reduce energy consumption Effect

Inactive Publication Date: 2012-01-18
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] If too much water is added to oil cake materials such as linseed cake, it will ea

Method used

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  • Method for preparing oil cake protein feedstuff through low moisture solid state fermentation
  • Method for preparing oil cake protein feedstuff through low moisture solid state fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] like figure 1 Shown, a kind of method that low-moisture solid-state fermentation prepares oilseed meal protein feed, it comprises the steps:

[0038] (1) Preparation of bacterial agent: Pick 1 ring colony of Bacillus subtilis from the inclined surface and transfer it to a 250ml triangular flask containing 100ml of glucose beef extract peptone liquid medium, and then place the triangular flask on a shaker at 37°C , 160r / min shake flask culture for 12h (6 ~ 24h can be). Centrifuge the shake flask bacterial liquid to remove the supernatant, and collect the bacterial cells;

[0039] The collected bacteria are washed with sterile physiological saline, centrifuged again, and repeated washing and centrifuging three times to obtain the washed and centrifuged bacteria;

[0040] According to the volume ratio of the washed and centrifuged thalli to sterile saline = 1g: 100mL, the washed and centrifuged thalli were resuspended with sterile saline to obtain a bacterial content of ...

Embodiment 2

[0046] A method for preparing oilseed meal protein feed through low-moisture solid-state fermentation, which comprises the following steps:

[0047] (1) Preparation of bacterial agents: Pick colonies of Bacillus subtilis, Bacillus natto, Saccharomyces cerevisiae, and lactic acid bacteria from the slope and transfer them to the corresponding peptone liquid medium containing glucose beef extract and peptone liquid medium containing glucose beef extract , YPD liquid medium, and MRS liquid medium in the Erlenmeyer flasks (4 Erlenmeyer flasks), then place the Erlenmeyer flasks on a shaker at 37°C, and shake the flasks at 160r / min for 12h (6-24h can be used), respectively ( 4 Erlenmeyer flasks) were centrifuged to remove the supernatant, and the bacteria were collected (collecting 4 Erlenmeyer flasks to obtain mixed bacteria);

[0048] washing the collected bacteria with sterile physiological saline, and centrifuging again; repeating the washing and centrifuging three times to obtai...

Embodiment 3

[0056] A method for preparing oilseed meal protein feed through low-moisture solid-state fermentation, which comprises the following steps:

[0057] (1) Preparation of bacterial agents: Pick 1 ring of colonies from Candida and Saccharomyces cerevisiae from the inclined surface and transfer them to 250ml triangles containing 100ml of YPD liquid medium (glucose beef extract peptone liquid medium can also be used). Then place the triangular flask on a shaker at 28°C, and shake the flask at 160r / min for 12 hours (6-24 hours are acceptable), centrifuge the shake flask bacterial liquid to remove the supernatant, and collect the bacterial cells;

[0058] washing the collected bacteria with sterile physiological saline, and centrifuging again; repeating the washing and centrifuging three times to obtain the washed and centrifuged bacteria;

[0059] According to the volume ratio of the washed and centrifuged thalli to sterile saline = 1g: 500mL, the washed and centrifuged thalli were r...

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Abstract

The invention belongs to the technical field of microbial fermentation, specifically relates to a method for preparing an oil cake protein feedstuff through low moisture solid state fermentation. The method for preparing the oil cake protein feedstuff through the low moisture solid state fermentation is characterized in that: the method comprises the following steps: 1) preparing a bacterial inoculum: injecting a mixed solution (comprising a bacterial liquid and a sodium alginate solution) in a CaCl2 solution through an injector, washing three times through sterilized physiological saline after curing to obtain a microcapsule bacterial inoculum, wherein a volume ratio of the sodium alginate solution to the CaCl2 solution is 1:2-1:10; 2) mixing and fermenting cake base materials: uniformly mixing and stirring the microcapsule bacterial inoculum, oil cake and water, carrying out standing fermentation for 24-96 hours at a room temperature to obtain a fermented product; 3) adopting one of the following two methods: (1) carrying out drying, wherein the fermented product is dried after completing the fermentation to obtain the oil cake protein feedstuff; (2) directly carrying out vacuum packaging for the fermented product after completing the fermentation to obtain the oil cake protein feedstuff. The method has characteristics of high survival rate and low production cost.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for preparing oil cake protein feed by adopting low-moisture solid-state fermentation. Background technique [0002] Cake-type feed refers to the remaining products after the oil seeds are squeezed or extracted to extract most of the oil. The remaining part after pressing and extracting oil is called cake, and the remaining part after pre-pressing and leaching is called meal. It mainly includes soybean cake, cottonseed cake, rapeseed cake, peanut cake, flax cake, camellia seed cake, sunflower seed cake, etc. In recent years, under the increasingly tight supply of animal protein, the application of plant protein cake feed in animal feed has gradually increased. Cake feed has the characteristics of rich protein content, relatively balanced essential amino acid composition, low price, and wide sources, but it also contains a variety of anti-nutri...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K10/12A23K10/37
CPCY02P60/87
Inventor 黄凤洪钮琰星黄茜万楚筠江木兰李文林黄庆德邓乾春周浩宇胡双喜
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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