Method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals
A mixed fermentation and feed protein technology, which is applied in the field of feed protein preparation, can solve the problems of polluting coastal tidal flats and offshore waters, large steam consumption, and long heating time, so as to save the step of separating fat, improve protein utilization, and reduce The effect of feed costs
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Embodiment 1
[0022] Example 1: According to the mass ratio of 1:1, 1000 kg of minced tilapia scraps were mixed with 1000 kg of soybean meal crushed and passed through a 60-mesh sieve, and 200 kg of water were mixed together to obtain The mixed fermentation raw materials; then add 1 kg of Bacillus subtilis, 3 kg of feed yeast, 5 kg of Lactobacillus delbrueckii, 1 kg of protease, 0.1 kg of cellulase, and 0.3 kg of β-glucose to the mixed fermentation materials Carbohydrase, 0.5 kg β-mannan, 0.3 kg lipase, stir and mix evenly, start closed solid state fermentation in the fermentor, the fermentation temperature is controlled in the range of 30 ℃ ~ 50 ℃, and the fermentation raw materials are periodically aerated , Ventilate once every 3h, each aeration time is 10min, no aeration after 30 hours and ensure airtight anaerobic conditions, continue to ferment for 60h, then end the fermentation, after the fermentation, the product obtained after fermentation is dried, crushed, inspected and packaged ,...
Embodiment 2
[0023] Example 2: According to the mass ratio of 1:1, 1000 kg of minced tilapia scraps were mixed with 1000 kg of soybean meal crushed and passed through a 60-mesh sieve, and 200 kg of water were mixed together to obtain Mixed fermentation raw materials; then add 3 kg Bacillus licheniformis, 3 kg Saccharomyces cerevisiae, 5 kg Lactobacillus bulgaricus, 1 kg protease, 0.1 kg cellulase, and 0.3 kg β-glucan to the mixed fermentation materials Enzyme, 0.5 kg β-mannan, 0.3 kg lipase are stirred and mixed uniformly, and the closed solid-state fermentation is started in the fermentor. The fermentation temperature is controlled in the range of 30 ℃ to 50 ℃. The fermentation raw materials are aerated regularly at intervals. Ventilate once every 6 hours, each time for 10 minutes. After 36 hours, no aeration and ensure airtight anaerobic conditions. Continue fermentation for 48 hours and then end the fermentation. After the fermentation, the product obtained after fermentation is dried, cr...
Embodiment 3
[0024] Example 3: According to the mass ratio of 0.8:1, 800 kg of minced tilapia scraps were mixed with 1000 kg of soybean meal crushed and passed through a 60-mesh sieve, and 300 kg of water were mixed together to obtain The mixed fermentation materials; then add 5 kg of Bacillus subtilis, 3 kg of feed yeast, 5 kg of Lactobacillus delbrueckii, 1.5 kg of protease, 0.1 kg of cellulase, and 0.3 kg of β-glucose to the mixed fermentation materials. Carbohydrase, 0.5 kg β-mannan, 0.2 kg lipase, stir and mix evenly, start closed solid-state fermentation in the fermentor, the fermentation temperature is controlled in the range of 30 ℃ ~ 50 ℃, and the fermentation raw materials are periodically aerated Ventilate once every 3h, each aeration time of 10min, no ventilation after 30 hours and ensure airtight anaerobic conditions, continue the fermentation for 60h, then end the fermentation, after the fermentation, the product obtained after fermentation is dried, crushed, inspected and pack...
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