Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds
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A technology of solid-state fermentation and lotus seeds, applied in botany equipment and methods, soilless cultivation, cultivation, etc., can solve the problems of low health care value, achieve simple production process, easy large-scale production and promotion, high edible and medical value effect
Active Publication Date: 2012-03-28
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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However, the culture medium has an important impact on the health value of Cordyceps. Many consumers are worried that the "Cordyceps flower" grown on the...
Method used
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Embodiment 1
[0012] Pick one loop of the Cordyceps militaris test tube strain and inoculate it into the Cordyceps seed medium (5.0% sucrose, 3.0% corn steep liquor, 0.5% yeast extract, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 0.05%), 25°C, 100r / min, cultured for 72 hours to obtain the Cordyceps liquid strain;
[0013] (1) Add 100g lotus seeds and 100mL distilled water to a 500mL triangular flask, seal with 8 layers of gauze, cook at 121°C for 20 minutes under high pressure, and use the steamed lotus seeds as a solid-state fermentation medium for Cordyceps;
[0014] (2) Aseptically inoculate 5mL of Cordyceps liquid strain; culture it for 10 days at a temperature of 25°C, a humidity of 80%, and in the dark to obtain lotus seeds covered with mycelium, Cordyceps,
[0015] (3) The Cordyceps lotus seeds covered with Cordyceps mycelium continue to be cultivated for 20 days at a temperature of 20°C and a humidity of 90%, with 12 hours of light per day, and fruiting bodies can be grown, that is, Cordycep...
Embodiment 2
[0018] Inoculate one loop of Cordyceps sinensis test tube bacteria into the seed medium (5.0% sucrose, 3.0% corn steep liquor, 0.5% peptone, 0.5% yeast extract, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 0.05%), 22°C, 60r / min, cultivated for 96h to obtain the liquid strain of Cordyceps sinensis;
[0019] (1) Add 100g lotus seeds and 100mL distilled water to a 500mL triangular flask, seal with 8 layers of gauze, cook at 121°C for 20min under high pressure, and use the steamed lotus seeds as a solid-state fermentation medium for Cordyceps;
[0020] (2) Aseptically inoculate 5mL of Cordyceps liquid strain; culture for 15 days under the conditions of temperature 20°C, humidity 80%, and darkness, to obtain Cordyceps lotus seeds covered with Cordyceps sinensis hyphae,
[0021] (3) Dry with hot air at 60°C for 20 hours; after packaging, it is the finished product of Cordyceps sinensis and lotus seeds.
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Abstract
The invention provides a method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds. The method comprises the following steps: on the basis of utilizing the steamed lotus seeds as a worm grass solid state fermentation culture medium, inoculating 3-8ml worm grass liquid strain to per 100 g of the lotus seeds; culturing for 10-30 days at the temperature of 5-30 DEG C and the humidity of 60-90% in dark, namely obtaining worm grass lotus seeds on which hyphae are overgrown; and culturing the worm grass lotus seeds on which the hyphae are overgrown at the temperature of 5-30 DEG C and the humidity of 80-95% for 10-30 days with the illumination of 8-15 hours per day, thus obtaining the worm grass lotus seeds on which worm grass sporocarps are grown. The invention provides a new worm grass source and a new lotus seed health food. According to the invention, the medical effect of worm grass and the nutrition of the lotus seeds are organically combined, so that the product has various nutrient components, is especially rich in active components such as cordycepin, a cordycepic acid, a worm grass polyose and the like and has high edible and medical values; and the worm grass lotus seed fungus preparation process is simple and can easily realize large-scale production, popularization and implementation.
Description
technical field [0001] The invention belongs to the field of health food processing methods, in particular to a method for producing Cordyceps lotus seeds by solid-state fermentation of lotus seeds. Background technique [0002] Cordyceps sinensis and Cordyceps militaris are the two most common Cordyceps fungi with high medicinal value in my country. Cordyceps ( Cordyceps sinensis (Berk.) Sacc. ) is Polychaete spp. ( Hirsutella hapial: chen et Shen, SP. nov ) infects bat moths of the order Lepidoptera ( Hepialus armoranus Oberthlür ) larvae, a complex of fungal sub-seats and dead larvae filled with mycelium develops, which is mainly distributed in alpine grasslands and alpine shrubs at an altitude of 3000-5000m. Cordyceps militaris ( Cordyceps militaris ), also known as Cordyceps militaris, is a model species of the genus Cordyceps with a wide distribution. The culture technology of Cordyceps militaris is relatively mature now. The artificially cultivated fruiting bo...
Claims
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Application Information
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IPC IPC(8): A01G31/00
Inventor 付湘晋
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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