Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds
A solid-state fermentation, lotus seed technology, applied in the directions of botanical equipment and methods, soilless cultivation, cultivation, etc., can solve problems such as low health care value, achieve simple production process, high edible and medical value, easy to large-scale production and promotion effect of implementation
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Embodiment 1
[0012] Pick one loop of the Cordyceps militaris test tube strain and inoculate it into the Cordyceps seed medium (5.0% sucrose, 3.0% corn steep liquor, 0.5% yeast extract, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 0.05%), 25°C, 100r / min, cultured for 72 hours to obtain the Cordyceps liquid strain;
[0013] (1) Add 100g lotus seeds and 100mL distilled water to a 500mL triangular flask, seal with 8 layers of gauze, cook at 121°C for 20 minutes under high pressure, and use the steamed lotus seeds as a solid-state fermentation medium for Cordyceps;
[0014] (2) Aseptically inoculate 5mL of Cordyceps liquid strain; culture it for 10 days at a temperature of 25°C, a humidity of 80%, and in the dark to obtain lotus seeds covered with mycelium, Cordyceps,
[0015] (3) The Cordyceps lotus seeds covered with Cordyceps mycelium continue to be cultivated for 20 days at a temperature of 20°C and a humidity of 90%, with 12 hours of light per day, and fruiting bodies can be grown, that is, Cordycep...
Embodiment 2
[0018] Inoculate one loop of Cordyceps sinensis test tube bacteria into the seed medium (5.0% sucrose, 3.0% corn steep liquor, 0.5% peptone, 0.5% yeast extract, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 0.05%), 22°C, 60r / min, cultivated for 96h to obtain the liquid strain of Cordyceps sinensis;
[0019] (1) Add 100g lotus seeds and 100mL distilled water to a 500mL triangular flask, seal with 8 layers of gauze, cook at 121°C for 20 minutes under high pressure, and use the steamed lotus seeds as a solid-state fermentation medium for Cordyceps;
[0020] (2) Aseptically inoculate 5mL of Cordyceps liquid strain; culture for 15 days under the conditions of temperature 20°C, humidity 80%, and dark conditions, and Cordyceps lotus seeds covered with Cordyceps sinensis hyphae can be obtained.
[0021] (3) Dry with hot air at 60°C for 20 hours; after packaging, it is the finished product of Cordyceps sinensis and lotus seeds.
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