Process for producing beta-thymidine through microbiological fermentation method

A microbial fermentation method and production process technology, applied in the field of fermentation engineering, can solve the problems of high cost and expensive raw materials, and achieve the effect of low cost, few fermentation stages and high cost

Inactive Publication Date: 2016-09-21
焦作健康元生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation method is still in the stage of laboratory research and development, the raw materials used are expensive, the cost is high, and industrialization has not yet been formed in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A production process for producing β-thymidine by microbial fermentation, comprising the following steps,

[0030] (1) Bacteria culture

[0031] a1. Inoculate the bacteria in Petri dish A containing the culture medium, and incubate at 37°C for 22 hours; continue to inoculate the mature strains in Petri dish B, and incubate at 37°C for 14 hours;

[0032] b1. Rinse the Petri dish B with physiological saline to obtain a bacterial solution and set it aside;

[0033] (2) Seed cultivation

[0034] a2. Add appropriate amount of water to the seed tank, followed by adding yeast extract powder 15 g / L, sodium chloride 8 g / L, potassium dihydrogen phosphate 3 g / L, dipotassium hydrogen phosphate 3 g / L, ammonium sulfate 3 g / L, glycerin 30 g / L, polyether 0.3 g / L, stir and mix;

[0035] b2. Adjust the pH to 7.2 with lye, and sterilize under high temperature and high pressure;

[0036] c2. Lower the temperature of the tank to 35°C, and use the differential pressure method to introdu...

Embodiment 2

[0043] A production process for producing β-thymidine by microbial fermentation, comprising the following steps,

[0044] (1) Bacteria culture

[0045] a1. Inoculate the bacteria in Petri dish A containing the culture medium, and incubate at 37°C for 22 hours; continue to inoculate the mature strains in Petri dish B, and incubate at 37°C for 16 hours;

[0046] b1. Rinse the Petri dish B with physiological saline to obtain a bacterial solution and set it aside;

[0047] (2) Seed cultivation

[0048] a2. Add appropriate amount of water to the seed tank, followed by adding yeast extract powder 15 g / L, sodium chloride 8 g / L, potassium dihydrogen phosphate 5 g / L, dipotassium hydrogen phosphate 5 g / L, ammonium sulfate 5 g / L, glycerin 40 g / L, polyether 0.5 g / L, stir and mix;

[0049] b2. Adjust the pH to 7.5 with lye, and sterilize under high temperature and high pressure;

[0050] c2. Lower the temperature of the tank to 40°C, use the differential pressure method to transfer th...

Embodiment 3

[0057] A production process for producing β-thymidine by microbial fermentation, comprising the following steps,

[0058] (1) Bacteria culture

[0059] a1. Inoculate the bacteria in Petri dish A containing the culture medium, and incubate at 37°C for 24 hours; continue to inoculate the mature strains in Petri dish B, and incubate at 37°C for 16 hours;

[0060] b1. Rinse the Petri dish B with physiological saline to obtain a bacterial solution and set it aside;

[0061] (2) Seed cultivation

[0062] a2. Add appropriate amount of water to the seed tank, followed by adding yeast extract powder 20 g / L, sodium chloride 8 g / L, potassium dihydrogen phosphate 5 g / L, dipotassium hydrogen phosphate 5 g / L, ammonium sulfate 5 g / L, glycerin 40 g / L, polyether 0.8 g / L, stir and mix;

[0063] b2. Adjust the pH to 7.8 with lye, and sterilize under high temperature and high pressure;

[0064] c2. Lower the temperature of the tank to 37°C, and use the differential pressure method to introdu...

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PUM

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Abstract

The invention discloses a process for producing beta-thymidine through a microbiological fermentation method. The process is characterized in that a strain culturing process, a seed culturing process and a fermental culturing process are carried out to obtain a fermenting liquid. According to the process, the fermenting conditions are optimized; the fermenting grades are decreased; in addition, the process is easily controlled; the formula of a culture substrate is adjusted; the fermenting cost is reduced. The process has the beneficial effects that the process is simple, the fermenting grades are decreased, the process is stable and easily controlled, the cost is low, and industrial production is facilitated.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular to a production process for producing β-thymidine by microbial fermentation. Background technique [0002] The full name of β-thymidine is β-2'-deoxythymidine, which is a natural compound and one of the four natural nucleosides that make up the biological macromolecule of deoxyribonucleic acid (DNA). It is now known that nucleosides and their deformed nucleosides play an extremely important role in cancer chemotherapy and as antiviral compounds. So far, most of the antiviral drugs approved internationally are nucleoside derivatives. β-thymidine is the ideal raw material for anti-HIV drugs AZT (azidovidine) and D4T (stavudine), and can also be used as a pharmaceutical intermediate for direct export, so the synthesis of β-thymidine is very important significance and economic value. [0003] At present, the production of thymidine mainly includes chemical synthesis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/38C12R1/19
CPCC12P19/385
Inventor 林楠棋张欣张书汁李占涛李兆如
Owner 焦作健康元生物制品有限公司
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