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Coenzyme Q10 fermentation process and control strategy

A technology of coenzyme and fermentation method, applied in the field of coenzyme Q10 fermentation process and control strategy, can solve the problems of long microbial fermentation process, influence judgment, low repeatability, etc., and achieve the effects of reducing fermentation cost, stabilizing production level, and improving fermentation level

Active Publication Date: 2015-04-29
INNER MONGOLIA KINGDOMWAY PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After determining the production strain and fermentation medium, the fermentation conditions need to be optimized. The shake flask and small tank test phase can be optimized by other statistical methods such as single factor. The situation is more complicated when using this method to optimize in production Moreover, the workload is large, the microbial fermentation process is long, and the fermentation results have certain volatility, which will cause certain interference to the final result analysis, affect judgment, low repeatability, and low efficiency.

Method used

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  • Coenzyme Q10 fermentation process and control strategy
  • Coenzyme Q10 fermentation process and control strategy
  • Coenzyme Q10 fermentation process and control strategy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] After the coenzyme Q10 preserved strain CGMCCNo.4497 was activated for three generations, a single colony with a round and full appearance, smooth edges and darker color on the plate was picked and diluted and spread on the plate medium for culture. The plate medium components are: glucose 2g / L, yeast powder 1g / L, peptone 1g / L, agar powder 2g / L, pH 6.8, and the culture conditions are 32°C for 5 days, until the appearance of the colonies on the plate is round and the edges are smooth , When the color is darker, it can be inserted into the mother bottle for cultivation.

[0036] Mother bottle medium components are: ammonium chloride 1.7g / L, peptone 0.7g / L, monosodium glutamate 0.4g / L, corn steep liquor 0.4g / L, glucose 2g / L, disodium hydrogen phosphate 0.35g / L, phosphoric acid Sodium dihydrogen 0.35g / L, ferric chloride 0.08g / L, magnesium sulfate 1.4g / L, potassium chloride 1.4g / L, copper sulfate 0.02g / L, zinc chloride 0.0006g / L, calcium bicarbonate 5.6g / L, auxiliary liquid...

Embodiment 2

[0047] After the coenzyme Q10 preserved strain CGMCCNo.4497 was activated for three generations, a single colony with a round and full appearance, smooth edges and darker color on the plate was picked, diluted and spread, and then inoculated on the plate medium for culture. The plate medium components are: glucose 3g / L, yeast powder 2g / L, peptone 2g / L, agar powder 2.5g / L, pH 7.0. The culture condition is 32°C for 6 days, and when the appearance of a single colony is round, the edge is smooth, and the color is dark, it can be inserted into the mother bottle for culture.

[0048] Mother bottle medium components are: ammonium chloride 2.1g / L, peptone 0.11g / L, monosodium glutamate 0.8g / L, corn steep liquor 0.8g / L, glucose 4g / L, disodium hydrogen phosphate 0.45g / L, phosphoric acid Sodium dihydrogen 0.45g / L, ferric chloride 0.1g / L, magnesium sulfate 1.8g / L, potassium chloride 1.8g / L, copper sulfate 0.04g / L, zinc chloride 0.001g / L, calcium bicarbonate 6.4g / L, auxiliary liquid I. Am...

Embodiment 3

[0059] After the coenzyme Q10 preserved strain CGMCCNo.4497 was activated for three generations, a single colony with a round and full appearance, smooth edges and darker color on the plate was picked and diluted and spread on the plate medium for culture. The plate medium components are: glucose 4g / L, yeast powder 3g / L, peptone 3g / L, agar powder 3g / L, pH 7.2. The culture condition is 32°C for 5 days, and when the appearance of a single colony is round and full, the edges are smooth, and the color is dark, it can be inserted into the mother bottle for culture.

[0060] Mother bottle medium components are: ammonium chloride 2.5g / L, peptone 1.5g / L, monosodium glutamate 1.2g / L, corn steep liquor 1.2g / L, glucose 6g / L, disodium hydrogen phosphate 0.55g / L, phosphoric acid Sodium dihydrogen 0.55g / L, ferric chloride 0.12g / L, magnesium sulfate 2.2g / L, potassium chloride 2.2g / L, copper sulfate 0.06g / L, zinc chloride 0.0014g / L, calcium bicarbonate 7.2g / L, auxiliary liquid I. Among them...

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Abstract

The invention provides a coenzyme Q10 fermentation process and a control strategy. Rhodobacter sphaeroides fermentation is adopted to produce coenzyme Q10, a specific operation method of coenzyme Q10 fermentation is provided, a process regulation and control standard is created, through bacterial type change in observation of the fermentation process, the bacterial type transition period is taken as a basis for judging time and amplitude for process parameter adjustment, the fermentation level of the coenzyme Q10 is greatly improved, the production level is stabilized, and the fermentation cost is reduced.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a coenzyme Q10 fermentation process and control strategy. Background technique [0002] Coenzyme Q10 is combined on the mitochondrial membrane in the cells of animals, plants and microorganisms. It is an important hydrogen transporter in the respiratory chain, an effective biochemical drug, and has many important physiological functions. A number of data prove that coenzyme Q10 is becoming more and more important to human health and disease treatment. Recent studies have found that coenzyme Q10 has anti-aging effects, and it has been widely used in the field of cosmetics and health products, and the demand at home and abroad has further expanded. [0003] The production methods of coenzyme Q10 mainly include animal and plant tissue extraction, chemical synthesis and microbial fermentation. Among them, the cost of animal and plant extraction is too high and is not suitable f...

Claims

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Application Information

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IPC IPC(8): C12P7/66C12R1/01
CPCC12P7/66
Inventor 朱志春蒋四富陈必钦文昌张广忠
Owner INNER MONGOLIA KINGDOMWAY PHARMA
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