Serum or plasma miRNA marker related to diabetes mellitus type 2 and application thereof

A type 2 diabetes marker technology, applied in the field of serum or plasma miRNA markers, can solve the problems that tissue miRNA cannot meet the needs of early diagnosis, cannot be applied to screening and prevention, etc., achieve easy separation and extraction, improve sensitivity and specificity , pain relief effect

Active Publication Date: 2016-11-23
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although miRNA in tumor tissue can be used as a new tumor marker, miRNA in tumor tissue must be obtained through surgery or puncture, so tissue miRNA cannot meet the needs of early diagnosis and cannot be used for extensive screening and prevention.

Method used

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  • Serum or plasma miRNA marker related to diabetes mellitus type 2 and application thereof
  • Serum or plasma miRNA marker related to diabetes mellitus type 2 and application thereof
  • Serum or plasma miRNA marker related to diabetes mellitus type 2 and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Test materials: Trizol reagent, TaqMan reversal rate kit, UniversalMaster Mix II was purchased from Life technologies company; chloroform and absolute ethanol were purchased from Sinopharm Group Pharmaceutical Co., Ltd.; DEPC water was purchased from Shanghai Beyontian Biotechnology Co., Ltd.

[0045] 2. Sample collection and sample data collation:

[0046] The present invention uses a total of 352 children's blood samples, including 136 samples of the obese group and 216 samples of the normal group; there is no significant difference in age between the two groups of samples (P>0.05), and there is a significant difference in BMI (P<0.001). There were significant differences in blood lipid, total cholesterol, low-density lipoprotein and high-density lipoprotein (P<0.05).

[0047] The present invention uses a total of 101 samples of adult type 2 diabetes and 82 samples of the control group; there is no significant difference in age between the two groups of samples, ...

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Abstract

The invention discloses a serum or plasma miRNA marker related to diabetes mellitus type 2 and an application thereof. The invention also discloses a diagnosis kit for diabetes mellitus type 2. The marker disclosed by the invention diagnoses fat children from a microscopic view and warns the risk of long-term insulin resistance and diabetes mellitus type 2; the operation is easy, the result is accurate and objective and reliable, the diabetes mellitus type 2 group and the normal metabolism group can be perfectly distinguished, and the risk of long-term insulin resistance and diabetes mellitus type 2 of fat children can be accurately and sensitively evaluated and warned; and only a blood sample is required instead of other tissue samples, thus the possibility and feasibility of clinical application are greatly improved, and an guidance basis is provided for clinical doctors to make early prevention, intervention schemes and judgment prognosis.

Description

technical field [0001] The invention relates to the fields of genetic engineering and medicine, in particular to a serum or plasma miRNA marker related to type 2 diabetes and its application. Background technique [0002] Diabetes mellitus is a chronic systemic metabolic disease characterized by persistent high blood sugar as its basic biochemical feature. a syndrome. Diabetes is mainly divided into type 1 diabetes, type 2 diabetes, gestational diabetes and other specific types of diabetes. Among them, type 1 diabetes is mainly determined by genetic factors, accounting for 5%-10%; type 2 diabetes is caused by the inability of the human body to effectively synthesize and secrete insulin, mainly due to overweight and lack of physical activity, accounting for about 90%; my country The proportion of diabetes is similar to that in the world. According to the statistics of the World Health Organization, there were 120 million diabetic patients in the world in 1998, and it is exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 季晨博崔县伟郭锡熔王兴尤梁惠文娟
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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