Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for distinguishing fermented dannanxing processed from pig bile and mixed steamed dannanxing

A technology of dannanxing and hyocholic acid, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of unidentified dannanxing, etc.

Active Publication Date: 2020-10-30
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +1
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no relevant research on the use of different bile acid components to identify different processed products of Dannanxing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for distinguishing fermented dannanxing processed from pig bile and mixed steamed dannanxing
  • A method for distinguishing fermented dannanxing processed from pig bile and mixed steamed dannanxing
  • A method for distinguishing fermented dannanxing processed from pig bile and mixed steamed dannanxing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1 Instruments and reagents

[0017] Silica gel G thin-layer plate was purchased from Qingdao Ocean Chemical Factory; Milli-Q pure water instrument; BP211D electronic balance (Germany Sartorius); KQ-500DE medical numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0018] Waters Alliance 2695HPLC (Waters Company, USA), equipped with evaporative light scattering detector (ELSD2000ES, Alltech Company), Empower chromatography workstation.

[0019] Reference substance: hyodeoxycholic acid (HCA) was purchased from Zhenzhun Biology; hyodeoxycholic acid reference substance (Hyodeoxycholic acid, HDCA, greater than 98%), chenodeoxycholic acid (Chenodeoxycholic acid, CDCA, greater than 98%), glycine Hyodeoxycholic acid (Glycohyodeoxycholic acid, GHDCA, greater than 97%) and glycychenodeoxycholic acid (Glycochenodeoxycholic acid, GCDCA, greater than 97%) had a purity greater than 98%, purchased from Shanghai Yuanye Biotechnology Co., Ltd.; taurine Chol...

Embodiment 2

[0043] The invention provides a high-performance liquid chromatography identification method for identifying fermented and mixed-steamed dannanxing prepared from pig bile, and its application in identifying different processed products of dannanxing. There are two kinds of processing methods of the dannanxing, namely fermentation method and mixed steaming method, and the processed products are respectively fermented and mixed steamed dannanxing.

[0044] Using high performance liquid chromatography (HPLC), the bile acid components hyodeoxycholic acid (HDCA), chenodeoxycholic acid (CDCA) and glycine hyodeoxycholic acid (GHDCA), glycochenodeoxycholic acid were established. (GCDCA) qualitative analysis method. Results: 5 standard substances of bile acid components could be completely separated. figure 2 , under the same conditions, in the chromatograms of the Dannanxing samples produced by the three manufacturers of QY, FZ, and QF, there are bile acid components such as hyochol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed is an identification method for fermented and mixed-steamed bile arisaema processed from porcine bile. The identification method includes: taking hyocholic acid (HCA), hyodeoxycholic acid (HDCA) and chenodeoxycholic acid (CDCA) as a group of markers, taking glycohyodeoxycholic acid (GHDCA) and glycochenodeoxycholic acid (GCDCA) as another group of markers, and applying the groups of markers in identifications of the fermented and mixed-steamed bile arisaema processed from the porcine bile. According to the identification method, thin-layer chromatography and high-performance liquid chromatography are provided to identify bile acid ingredients in the bile arisaema processed from the porcine bile, so that different products of the bile arisaema processed from the porcine bile can be identified simply and quickly, and quality of the bile arisaema can be controlled better. The identification method has the advantages of high specificity and good reproducibility.

Description

technical field [0001] The invention belongs to the field of quality evaluation of Chinese herbal decoction pieces, and in particular relates to a method for distinguishing fermented pig bile and mixed steamed dannanxing. Background technique [0002] Dannanxing is a traditional fermented preparation with a long history of blind application, processed through certain procedures, and has been used for generations until now. It is one of the commonly used clinical medicines. "Bannanxing" recorded in "Chinese Pharmacopoeia" (2015 edition) is made from the fine powder of arachis and the bile of cattle, sheep or pigs, or from the fine powder of raw arias and the bile of cattle, sheep or pigs through fermentation . It has the functions of clearing away heat and resolving phlegm, subduing wind and relieving convulsions, and is used for cough due to phlegm heat, thick yellow phlegm, apoplectic phlegm obsession, mania and convulsions. Modern pharmacological studies have shown that ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/02
CPCG01N30/02G01N30/90G01N2030/042
Inventor 吴皓郁红礼茅仁刚陈劲松陈云代向向
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com