Preparation method of enzyme induced bezoar

A bezoar and enzyme solution technology, applied in medical preparations containing active ingredients, pharmaceutical formulations, unknown raw materials, etc., can solve the problems of poor curative effect and unsatisfactory effect, and achieve rich raw materials, low cost and large output. Effect

Inactive Publication Date: 2009-07-08
刘化侠
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AI Technical Summary

Problems solved by technology

For this reason, scientific researchers have been studying the substitutes of bezoar for a long time. Although certain results have been obtained, the effect is not satisfactory

Method used

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Embodiment Construction

[0010] The present invention is a method for preparing bezoar with enzymes. According to the cause of bezoar, in vitro, Escherichia coli is used to produce β-GA enzyme, combined with the ancestral secret recipe and borax, and the bile of animals such as cattle, sheep, or pigs is used as raw materials. Through the enzymatic reaction, bezoar can be mass-produced. That is: in vitro, culture Escherichia coli, extract β-GA enzyme from the E. coli cell, then add β-GA enzyme to the bile of cattle, sheep, or pigs, and catalyze the catalysis of cattle, sheep and other animals through enzymatic reactions. The active ingredients of bile bezoar of animals such as pigs and pigs are all concentrated to prepare bezoar.

[0011] Process of the present invention is:

[0012] 1. Cultivate Escherichia coli: Culture Escherichia coli in 3.6-4.0% protein broth medium, keep the temperature at 35-38°C for 24 hours, centrifuge at 5000r / min for 18-22 minutes, discard the supernatant, after precipitati...

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Abstract

The invention relates to a method for preparing enzyme-natured bezoar. In the method, colon bacillus is natured in vitro; colon bacillus beta-GA enzyme is extracted and implanted to the gall of animals, such as cattle, sheep, pig, etc; the effective ingredients of gall bezoar are catalyzed and totally precipitated, then the bezoar is prepared. Namely: the colon bacillus is natured in 3.6-4.0% of albumen brothculture at 35-38 DEG C of constant temperature for 24 hours and centrifugally precipitated, then colon bacillus precipitation is gained; then 10-15g of the colon bacillus precipitation is added into 400mlpH6.8 of trihydroxymethyl aminomethane-hydrochloric acid buffer solution, vibrated in an ultrasonic groove for 15-20 minutes and centrifuged, then the colon bacillus beta-GA enzyme solution is gained; 28-32ml of the solution is added into 3,000ml of the gall of the animals, such as cattle, sheep, pig, etc, and 20-30g of borax is added, enzymatic reaction is carried out on the mixture at 35-38 DEG C of constant temperature for 24 hours, the bezoar of the invention is gained. The invention is characterized in that the raw materials are abundant, the technique is simple, the equipment is less, the investment is less, the cost is low, the benefit is high; the therapeutic effect of the enzyme-natured bezoar is the same as or better than that of the natural bezoar; and the enzyme-natured bezoar can be prepared industrially.

Description

1. Technical field: [0001] The invention relates to a preparation method of enzyme-educated bezoar, specifically a method for cultivating Escherichia coli in vitro according to the cause of bezoar, extracting Escherichia coli β-GA enzyme from the bacteria, and implanting it into the bile of cattle, sheep, pigs and other animals. Among them, the method for preparing bezoar by catalyzing the precipitation of active ingredients in bile bezoar of cattle, sheep, pigs and the like. 2. Background technology: [0002] Bezoar is a famous and precious traditional Chinese medicine. Its curative effect is miraculous but strange. There is a saying that "gold is easy to get, but bezoar is hard to find". It is reported that my country's traditional Chinese medicine needs 5 tons of bezoar every year as the main ingredient of more than 600 kinds of Chinese patent medicines such as Angong Niuhuang Pills; the actual demand is about 200 tons. It is estimated that my country's annual output of ...

Claims

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Application Information

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IPC IPC(8): A61K35/413
Inventor 周和亭刘化侠高允生赵晓民周春国孙兆会
Owner 刘化侠
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