Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quantification of 16 bile acids in bile and serum based on LC-MS/MS

A bile acid and serum technology, applied in the field of mass spectrometry detection, can solve problems such as difficulty in simultaneous separation and detection

Inactive Publication Date: 2018-01-23
上海可力梅塔生物医药科技有限公司 +3
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the structural properties of bile acids and their conjugates are very different, for example, unconjugated bile acids have poor water solubility and pKa values ​​are generally between 3 and 5, while conjugated bile acids have better water solubility and lower pKa. Due to the low concentration of bile acids in serum and the possible presence of multiple isomers at the same time, it is very difficult to separate and detect them at the same time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quantification of 16 bile acids in bile and serum based on LC-MS/MS
  • Quantification of 16 bile acids in bile and serum based on LC-MS/MS
  • Quantification of 16 bile acids in bile and serum based on LC-MS/MS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Using the method as described in the present invention, the serum bile acid content of 63 normal people has been measured, see Table 5, including the maximum value and minimum value of the average value and 2.5%-97.5% normal population, which is consistent with the reference value reported in the literature The range is basically the same, indicating that the method is accurate. At the same time, in addition to being applicable to the detection of bile acids in serum, the method is also applicable to the detection of diluted bile samples, and has universal applicability.

[0057] Table 5 Serum bile acid assay results (n=63)

[0058]

[0059]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses quantification of 16 bile acids in the bile and serum based on LC-MS / MS. Specifically, the invention provides a detection kit for bile acids through a high performance liquid chromatography and mass spectrometry method. The kit comprises a quality control substance, an isotope internal standard extracted liquid, a diluent, a conversion liquid, a reconstitution fluid, a mobile phase additive A and a mobile phase additive B. The kit can be used for detecting 16 bile acids, respectively, and is less in time consumption and high in detection efficiency.

Description

technical field [0001] The invention relates to the field of mass spectrometry detection, in particular to various detection kits for bile acids by high performance liquid chromatography mass spectrometry. Background technique [0002] Bile acid is the product of cholesterol oxidation by cytochrome in the liver, and plays an important role in the digestion and absorption of fat in the intestine and the maintenance of cholesterol balance in the body. Bile acid is the main component of bile and one of the important physiological media in the human body environment, mainly including cholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid and ursodeoxycholic acid, and these five kinds of free bile acid counterparts glycine and taurine-binding bile acids. In the bile and peripheral blood of healthy people, the components of the bile acid spectrum are relatively constant; when various diseases such as cholelithiasis, atherosclerosis, and hypertension occur, it will...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 朱监宝官培龙郭孟磊
Owner 上海可力梅塔生物医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products