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Method for preparing probiotic brown lactobacillus beverage product with large amount of bacteria

A lactic acid bacteria beverage and probiotic technology, applied in dairy products, milk preparations, applications, etc., can solve the problems of poor tolerance, easy inactivation by gastric acid, and low number of probiotics, achieve strong tolerance, restore intestinal The effect of bacterial flora balance

Active Publication Date: 2010-06-16
SHANDONG DEYI DAIRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, after the product passes through the gastric acid of the human body, the number of probiotics that can survive is very small, and its probiotic effect cannot be maximized after reaching the intestinal tract
[0008] Keeping the viable count of probiotics in milk fermented products to the greatest extent has always been the focus of current research. In dairy products of the prior art, active bifidobacteria, Lactobacillus casei and other probiotics have poor tolerance to low pH values. , easy to be inactivated by gastric acid; extremely sensitive to oxygen, easy to inactivate under aerobic conditions, etc., which lead to a rapid decline in the number of viable bacteria, which needs to be solved urgently

Method used

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  • Method for preparing probiotic brown lactobacillus beverage product with large amount of bacteria
  • Method for preparing probiotic brown lactobacillus beverage product with large amount of bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 is prepared using skimmed milk powder

[0039] All raw materials: skimmed milk powder, glucose, purified water, Lactobacillus casei strains, citric acid, sucrose, fructose syrup, pectin and food flavors are commercially available.

[0040] Equipment for trial production: colloid mill or water-powder mixer, homogenizer, temporary storage tank, sterilizing plate combination, cooling plate, screw pump, constant temperature incubator, PH meter, viscometer and filling machine, etc.;

[0041] Ingredients:

[0042] Skimmed milk powder 10kg, glucose 2kg, purified water 70kg;

[0043] Specific steps:

[0044] 1. Hydration: Restore skim milk powder and purified water to skim milk at a ratio of 1:4~8, and add a certain amount of glucose and the rest of water for hydration. The hydration temperature is 40~50°C, and the hydration time is 30~40min; stirring time And frequency: 30s / 15min; after the hydration is completed, sterilize at 95°C for 5min; obtain the steriliz...

Embodiment 2

[0055] Embodiment 2 is prepared using skimmed milk powder

[0056] 1. Maillard reaction

[0057] Skimmed milk powder 20kg, glucose 6kg, purified water 160kg;

[0058] Hydration temperature: 50-55°C, hydration time: 40-50min; stirring time and frequency: 30sec / 15min; sterilization temperature: 98°C, 5min; stir at 95-98°C, react for 2.0-2.5h; the reaction liquid changes from milky yellow to When the brown color is the end point of the reaction, the Maillard reaction solution is obtained and set aside;

[0059] 2. Probiotic fermentation

[0060] Cool the Maillard reaction solution to 37-38°C, inoculate 0.02-0.03 g of bacteria per 100ml of Maillard reaction solution, inoculate Lactobacillus casei (Lactobacillus casei) bacteria, stir for 10-15 minutes after inoculation, and then inoculate at 37 Ferment at ~38°C for 72 hours. When the pH reaches 3.8-4.0, stop the fermentation, cool to 2-6°C, and obtain the probiotic fermentation broth for later use;

[0061] 3. Post-processing ...

Embodiment 3

[0066] Embodiment 3 is prepared using skimmed milk powder

[0067] Maillard reaction

[0068] Weight ratio: skim milk powder 15kg, glucose 4kg, purified water 120kg; inoculate 0.02-0.025g of bacteria per 100ml of Maillard reaction solution, inoculate Lactobacillus casei (Lactobacillus casei) bacteria, probiotics ferment for 56 hours, and adjust the acidity tartaric acid;

[0069] During base material preparation, beet sugar 4kg, fructose syrup 8kg, pectin 0.4kg, purified water 80kg, all the other are the same as embodiment 2.

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Abstract

The invention discloses a method for preparing a probiotic brown lactobacillus beverage product with large amount of bacteria, which comprises the steps of Maillard reaction, probiotic fermentation and post treatment, wherein the probiotic fermentation step comprises: hydrating and sterilizing defatted milk powder or defatted fresh milk, reacting the defatted milk powder or the defatted fresh milk for 2.5 hours at the temperature of between 95 and 98 DEG C till the milky yellow becomes brown at the end point of the reaction, cooling the reaction product to between 37 and 38 DEG C, inoculating the reaction product to a Lactobacillus casei strain, statically fermenting the strain for 48 to 72 hours at the temperature of between 37 and 38 DEG C till the pH reaches 3.8 to 4.0, and cooling the fermentation product to between 2 and 6 DEG C; and the post treatment steps comprises: preparing base solution by using sugar, high fructose corn syrup, pectin and purified water, wherein the weight ratio of the base solution to the probiotic fermentation solution is 1 to 3; and the pH is adjusted to between 3.5 and 3.6; homogenizing the base solution, cooling the base solution to between 2 and 6 DEG C, canning products after passing detection, maturing the products for 4 to 8 hours at the temperature of between 2 and 6 DEG C, and delivery the products from a storage. The number of the living bacteria in the shelf life of the product can reach 1*108 to 1*1,010cfu / ml; and the product has zero fat and super clear mouthfeel, has no viscosity, has high probiotic detection rate, and can effectively keep or recover the intestinal microbial balance.

Description

technical field [0001] The invention belongs to the technical category of milk products, and in particular relates to a preparation method of a probiotic brown lactic acid bacteria beverage product with a high bacterial count. Background technique [0002] With the development and progress of society, people's life expectancy continues to increase; at the same time, people are more pursuing quality of life, longevity requires health, and a well-functioning gastrointestinal tract is the foundation of health. If probiotics are taken regularly, they can effectively maintain or restore the balance of intestinal flora. [0003] According to the definition of Food and Agriculture Organization of the United Nations / World Health Organization (FAO / World Health Organization for short) in 2002, probiotics are living microorganisms that, when ingested in sufficient quantities, produce one or Multiple proven functional health benefits. [0004] Probiotics have the functions of regulati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/12
Inventor 孙晶王艳丁玉振周春艳
Owner SHANDONG DEYI DAIRY IND
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