60 results about "Plant phenotyping" patented technology

The invention relates generally to an improved plant breeding system. More particularly, this invention relates to a method for automated, high throughput analysis of plant phenotype and plant genotype in a breeding program.

The invention discloses a high-flux greenhouse plant phenotype measurement system based on multispectral point cloud fusion. TOF cameras and multispectral cameras are arranged under multiple perspectives, three-dimensional point cloud and multispectral images under the multiple perspectives are gathered, registration is carried out between a multispectral image and a three-dimensional point cloudimage, unified transformation of multi-perspective coordinates is carried out, multi-perspective multispectral three-dimensional point cloud fusion is realized, high-flux greenhouse plant phenotype measurement based on the multispectral three-dimensional point cloud model is realized, measurement on the plant external phenotype (three-dimensional morphological information) and the internal phenotype (physiological information) is realized, and the high-flux greenhouse plant phenotype measurement system has the advantages of high precision, fast speed and strong applicability.

Systems, methods, and apparatus for large scale, high throughput phenotyping of items including plants to derive environment/genetics correlations based on controlled variation of environment. Spatial and temporal resolution is improved by an integrated system that can use space efficiently yet concurrently process a large number of replicates but in varying environments. This is accomplished by using an array of miniature greenhouses, each in communication with one or more generators or regulators of an environmental factor that can be independently supplied and controlled to each miniature greenhouse. The controller also controls acquisition of data from each greenhouse as well as processing of that data into phenomic/genomic correlations.

A unique fusion gene is disclosed which is useful for transforming a wide range of plants, resulting in a significant alteration of the plant phenotype with respect to shoot and floral tissue response, but not affecting root growth or function. The gene construct includes an ACC oxidase promoter to drive an ipt coding sequence that expresses IPT at certain stages of plant maturation and in certain tissues of the shoot. Exemplary transformations include chrysanthemum and tobacco, both of which exhibit increased branching in the vegetative shoot and increased bud count in the generative shoot.

The invention discloses a mobile phenotypic cabin for acquiring and analyzing phenotypes of field crops. The mobile phenotypic cabin comprises a root system monitoring system, a phenotypic cabin framesystem, an environment control system and a data acquisition system. An operating mechanism is arranged on the phenotypic cabin frame system, so that the phenotypic cabin can move back and forth on afield to a proper position, and contrast experiments of different environmental factors are carried out on the field under the same external soil hydrological environment. In the experiment process,an internal environment of the phenotypic cabin frame system can be set to be closed and controllable through a water sealing device, so that the adjustment of environmental factors in the cabin is more accurate, the phenotypic characteristic observation of indoor and outdoor plants can be realized, and the phenotypic characteristic acquisition of overground and underground plants is realized. Dueto the fact that field crop samples are rich, high-throughput, high-precision, low-cost and comprehensive phenotypic acquisition and analysis of crops can be carried out.

Unique fusion genes are disclosed which are useful for transforming a wide range of plants, and when used in tandem, result in a significant alteration of the plant phenotype with respect to tolerance to the stress from prolonged storage under either dark or cold conditions, or a combination of cold and dark conditions. With intact plants such as transplants, plants harboring these genes maintain the ability of recover and grow normally after returned to normal growth conditions. With isolated plant parts, such as cut flowers or foliage or fruits & vegetables, leaf tissue maintains color quality and cells maintain structural integrity during prolonged storage. Since the transgenes are only activated in response to cold temperature, normal plant growth, development, and function is not affected. The gene constructs include (1) a cold-regulated gene (COR15a) promoter to drive an ipt coding sequence that expresses IPT in the tissues of plants or plant parts exposed to a short cold induction period; and (2) a cold-regulated gene (COR15a) promoter to drive a (FAD7) coding sequence that expresses a fatty acid desaturase enzyme in the tissues of plants or plant parts exposed to a short cold induction period. Exemplary transformations include chrysanthemum, tobacco, flowering tobacco, and petunia. Double transgenic tobacco, and flowering tobacco both exhibited increased survival under prolonged cold and dark storage conditions.

The invention provides methods for constructing and predicting the leaf trait of a woody plant and a photosynthetic characteristic model based on a DNA methylation level, and belongs to the technicalfield of biological analysis. The predicting method comprises selecting important characteristic variable embodying a geographic position difference based on a random forest, screening out 7 leaf characteristic variables, determining an optimal cluster number, and obtaining each group of cluster leaf samples by using an improved FCM clustering algorithm; according to the correlation between variables and the importance of Enzyme digestion combination obtained by a gradient boosted tree, obtaining an important enzyme digestion combination in each group of cluster leaf samples; by using the DNAmethylation level of the enzyme digestion combination as a regression variable, constructing LS-SVM regression prediction model based on Gaussian radial basis function; inputting the DNA methylation level of important enzyme digestion combination to accurately predict a leaf shape factor, leaf area and a net photosynthetic rate. The method is used for predicting the phenotypic characteristic and the photosynthetic characteristic of the woody plant, and screening individuals of woody plants with excellent traits.

Systems and methods for plant phenotyping using mechanical manipulation are disclosed. In one embodiment, a method for plant phenotyping includes: acquiring a first image of a plant with a first imaging modality operating in a first spectrum; and acquiring a second image of the plant with a second imaging modality operating in a second spectrum. The first spectrum and the second spectrum have different depths of penetration through the plant. The method also includes analyzing the first image and the second image to determine the properties of the plant.

The disclosure provides methods and means for identifying plants comprising a plant phenotype of interest. In particular, the disclosure provides breeding tools that can be used for the selection of a plant comprising a phenotype of interest and for the selection of an optimal plant genotype for the introduction of a trait.

The present invention provides methods for identifying the nucleic acid sequences responsible for phenotypic traits in plants.

The invention relates to a device for extracting three-dimensional phenotypic information of small plants and seedling plants. The device comprises an upper computer control center, a precise motion controller and a visible light imaging module. The imaging module contains an image acquisition module, a loading rotary table and a visual light source. The precise motion controller has a single axismotion control function and can be communicated with the upper computer. The upper computer is mainly used for communicating with a lower computer and extracting three-dimensional information of theplant images. The device utilizes a machine vision technology and mechanical automation technology, realizes fast and non-destructive extraction of three-dimensional phenotypic information of small plants and provides a fast and efficient scientific and technological means for researching phenotypic characters of small plants or seedling plants for researchers in the related fields.

The invention relates to the technical field of plant phenotype monitoring equipment, and discloses a device and method for acquiring plant stem phenotype information. The device for acquiring the plant stem phenotype information comprises a helical track and a slide block, wherein an area enclosed by the helical track is used for placing a plant stem; the helical track comprises a conducting partand a guide rail part; the conducting part comprises two conducting sheets which are arranged on the two sides of the guide rail part oppositely; the guide rail part is used for insulating and isolating the two conducting sheets; the slide block is provided with a walking mechanism, an image acquiring mechanism and electric brushes which correspond to the conducting sheets one by one; the walkingmechanism is used for driving the slide block to move along the helical track; a lens of the image acquiring mechanism faces the inside of the helical track; the brush head ends of the electric brushes are connected to the conducting sheets in a sliding way; and the output ends of the electric brushes are electrically connected to the walking mechanism and the image acquiring mechanism. Accordingto the device for acquiring the plant stem phenotype information, the multiangle image acquisition of the plant stem is realized through the helical movement of the slide block, so that the manual intervention is reduced, and large-scale operation is facilitated.

The invention relates to the technical field of agriculture and forestry plant phenotypic characteristic information measuring equipment, in particular to a greenhouse high-throughput plant full-life-cycle phenotypic information measuring system and a greenhouse high-throughput plant full-life-cycle phenotypic information measuring method. A high-flux plant full-life-cycle phenotypic information measuring system is provided with an automatic collecting device capable of moving in a three-dimensional space, wherein the automatic collecting device comprises a grabbing device, a conveyor, an information collecting device and a controller. The controller is in signal connection with the grabbing device, the conveyor and the information collecting device. The conveyor is a belt conveyor, a chain plate conveyor or a roller conveyor, preferably belt conveyors and chain scraper conveyors, and further preferably belt conveyors. Herein, the conveying stability of the belt conveyor is superior to that of a chain plate conveyor, and the conveying stability of the chain plate conveyor is superior to that of a roller conveyor; and the power required by a driving motor on the belt conveyor is smaller than that required by the chain plate conveyor.

The invention provides a system for hydroponic plant growth environment regulation and control and phenotypic image acquisition. Nutrient solution is pumped into each hydroponic tank through respective independent connecting pipelines, and a plurality of mutually independent plant cultivation tank environments are respectively formed through the sealing effect of the hydroponic tanks and the transparent heat insulation covers. According to the system, the growth environment parameters of plants can be accurately regulated and controlled in real time through the sensors and the environment devices in the plant culture boxes, different culture conditions are simulated in the culture boxes, and research experiments of influences of different environment factors on plant growth and development are achieved under one system. On the basis, a plant phenotype image acquisition device comprising a multi-angle and multi-azimuth RGB camera group and a multi-spectral camera is also constructed outside the culture box, and can acquire multi-spectral images of plant stems and leaves and RGB images of the whole plant in the whole overground and underground range in a high-throughput manner on the premise of not influencing the growth and development of the plant; dynamic continuous collection of phenotypic characteristics of the overground part and the root system of the plant in different growth environments is realized.

The invention relates to an active vision platform for plant phenotypic acquisition and a plant phenotype acquisition method, wherein the vision platform comprises a depth camera and a workbench; thedepth camera is mounted on a bracket; a controller and a moving seat which are electrically connected with the depth camera are arranged on the workbench; a driving device for driving the moving seatto move toward a vertical axis of the depth camera is mounted on the workbench; a rotating table driven by a first stepping motor to rotate is arranged on the moving seat; and the first stepping motorand the driving device are electrically connected with the controller. According to the invention, the active vision platform is formed by a mechanical device and the depth camera, and the active vision platform is simple in structure and relatively low in cost; the controller realizes the entire active visual process based on an image acquired by the monocular camera and the accurate detection and feedback of a sensor, and has relatively high measurement precision; human involvement is reduced by an active visual approach, so that labor is saved; and the combination of a lifting device and the driving device ensures the measurement field of view of the depth camera.

The invention discloses a movable plant phenotype platform. The movable plant phenotype platform comprises a heat insulation tank body, wherein the inner part of the heat insulation tank body is divided into two parts of a temperature and humidity control system and a working region, wherein a horizontal guide rail is fixed to the top of the working region, a lifting mechanism is mounted on the horizontal guide rail, a horizontal movement driving motor drives the lifting mechanism to make horizontal movement along the guide rail, a visible light camera is mounted on the lifting mechanism, thelifting mechanism drives the visible light camera to move up and down, and a culturing frame is arranged below the horizontal guide rail. The temperature and humidity control system, a light supplementing lamp system and the heat insulation tank body jointly form an outside temperature and humidity and light environmental simulation system required for growth of plants. The lifting mechanism is arranged on the horizontal guide rail, and the lifting mechanism can be used for bearing the visible light camera, so that high-flux obtaining of plant phenotype information can be realized.

The invention discloses a mobile phenotype information acquisition platform and method for a whole plant growth cycle, and the platform comprises a foldable telescopic mechanical arm device, the foldable telescopic mechanical arm device comprises a self-propelled chassis device and a plant phenotype information acquisition device, and the plant phenotype information acquisition device is arranged on the self-propelled chassis device; the self-propelled chassis device is used as a carrier for moving the platform, so that the platform has better flexibility; the platform adopts the foldable telescopic mechanical arm, through the conversion of various states of the foldable telescopic mechanical arm, the requirement for collecting phenotype information of the whole period of plant growth in various scenes is met, the state of the foldable telescopic mechanical arm is automatically judged and accurately adjusted according to the growth stage of the plant, phenotype information collection is conducted, manpower is reduced, the working efficiency is improved, and the plant phenotype in-situ nondestructive collection is realized.

The invention provides a method and a system for acquiring outdoor plant phenotypic data. The method comprises the following steps of: constructing a windproof device for a to-be-detected plant; attaching scanning identification points to the to-be-detected plant establishing a plant space three-dimensional independent coordinate system according to the scanning identification points, and ensuringthat each working position of a scanner has three or more scanning identification points; carrying out a scanning pre-experiment, judging whether illumination affects scanning or not, and if so, adding a shading device in the incident direction of the sun; scanning the to-be-detected plant; and calculating each characteristic parameter of the to-be-detected plant according to the scanned data andthe plant space three-dimensional independent coordinate system. The technical problems of the influence of wind interference and high illumination intensity in a traditional handheld three-dimensional laser scanner used for scanning plant forms outdoors in the prior art are solved.

The invention relates to a wearable plant phenotype sensing system, including a computer and also a wearable device. The wearable device includes an image acquisition device for acquiring an image ofa plant and a data transmission device for transmitting the image of the plant to the computer. The computer includes a processor, a memory, and a program stored on the memory and executed by the processor, which, when executing the program, enables plant phenotypic information to be obtained from a plant image. Compared with the prior art, the invention combines the wearable equipment and the machine vision technology, and designs and realizes a wearable phenotypic sensing solution aiming at the plants under the actual natural scene. The system is portable, robust, reliable, and high in computational accuracy, which is conducive to popularization.

An outdoor high-throughput plant phenotypic information acquisition platform is convenient to move, the height is easy to adjust, and phenotypic information acquisition of plants with different heights in fields (fields and forests) of different places and in different growth periods can be met, suspension cable winding and unwinding devices for winding and unwinding suspension cables are arrangedon the four portable adjustable supporting rods located on the periphery. The suspension cable pull animal and plant phenotypic information acquisition devices are used for controlling the portable adjustable supporting rods to acquire the phenotypic information of the plants. A control instruction of the integrated control center is sent to the plant phenotype acquisition device in a wireless communication mode. The plant phenotype acquisition device acquires target plant phenotype data according to a selected mode (fixed-point hovering acquisition, block-by-block scanning acquisition and line-by-line scanning acquisition). Collected plant phenotypic data are sent to the integrated control center in a wireless communication mode to be stored, group data of large-scale plants are collected in real time, and high-throughput phenotypic parameter collection is achieved.

The invention discloses a plant phenotype acquisition device and method. The plant phenotype acquisition device comprises an imaging chamber, a rotating table, a camera module, a processor module and a control module, the processor module is respectively connected with the rotating table, the camera module and the control module; the imaging chamber forms a closed foldable imaging chamber through the telescopic rack module and the shading module; the rotating table is arranged in the imaging chamber and is used for fixing a plant to be detected and rotating the plant; the camera module is arranged above the interior of the imaging chamber and is used for collecting multi-azimuth image data of the plant to be detected; the processor module controls the rotary table to rotate according to a preset angle through the control module, and controls the camera module to start, process data and generate a three-dimensional point cloud, so that the processor module calculates phenotype data according to the three-dimensional point cloud; according to the plant phenotype acquisition method and device, the problems that the cost is high, the device is limited by sites, and point cloud noise and point cloud missing are likely to be caused in the three-dimensional reconstruction process are solved, and the plant phenotype acquisition method and device are economical, efficient, accurate and portable.