Methods for constructing and predicting leaf trait of woody plant and photosynthetic characteristic model based on DNA methylation level

Abstract

The invention provides methods for constructing and predicting the leaf trait of a woody plant and a photosynthetic characteristic model based on a DNA methylation level, and belongs to the technicalfield of biological analysis. The predicting method comprises selecting important characteristic variable embodying a geographic position difference based on a random forest, screening out 7 leaf characteristic variables, determining an optimal cluster number, and obtaining each group of cluster leaf samples by using an improved FCM clustering algorithm; according to the correlation between variables and the importance of Enzyme digestion combination obtained by a gradient boosted tree, obtaining an important enzyme digestion combination in each group of cluster leaf samples; by using the DNAmethylation level of the enzyme digestion combination as a regression variable, constructing LS-SVM regression prediction model based on Gaussian radial basis function; inputting the DNA methylation level of important enzyme digestion combination to accurately predict a leaf shape factor, leaf area and a net photosynthetic rate. The method is used for predicting the phenotypic characteristic and the photosynthetic characteristic of the woody plant, and screening individuals of woody plants with excellent traits.

Description

technical field [0001] The invention belongs to the field of biological information, and in particular relates to a construction method and a prediction method of a woody plant leaf phenotype characteristic and photosynthetic characteristic prediction model based on DNA methylation level. Background technique [0002] DNA methylation most commonly occurs at the fifth carbon atom of cytosine within a dinucleotide and is an important epigenetic modification. DNA methylation represses the expression of transposable elements, pseudogenes, repetitive sequences, and individual genes, and plays a key role in many biological processes such as gene expression, embryonic development, cell proliferation, differentiation, and chromosome stability. In plants, DNA methylation occurs at CG, CHG, CHH sites (H stands for bases other than guanine), and in higher plant cells, methylated cytosine can account for up to the total 50% of the number of cytosines. DNA methylation controls plant gr...

AI Technical Summary

Problems solved by technology

However, the research is still in the qualitative analysis, and there is a lack of quantitative research on DNA methylation analysis

At the same time, the current research on DNA methylation of forest plants relies on the scanning of DNA methylation of the whole genome, the research cost is high, and the huge amount of data leads to poor accuracy of results

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