Application of MuMADS2 to culturing of fruit quality-improved transgenic plant

A technology of transgenic plants and plants, applied in the fields of applications, plant peptides, plant products, etc., can solve the problems of MADS-box gene inactivation

Active Publication Date: 2014-12-03
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vrebalov et al. found that the rin mutant was obtained due to the deletion of a 3kb DNA in the tomato genome, resulting in the inactivation of two tandem MADS-box genes, namely LeMADS-RIN and LeMADS-MC

Method used

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  • Application of MuMADS2 to culturing of fruit quality-improved transgenic plant
  • Application of MuMADS2 to culturing of fruit quality-improved transgenic plant
  • Application of MuMADS2 to culturing of fruit quality-improved transgenic plant

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Experimental program
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Effect test

Embodiment 1

[0048] Embodiment 1, the acquisition of turning MuMADS2 tomato

[0049] 1. Acquisition of gene MuMADS2

[0050] The early postharvest suppression shrinkage library (SSH) of banana fruit was constructed, and a cDNA fragment homologous to MADS-box family genes was obtained. Design 5' and 3' end primers according to the sequence of the cDNA fragment, use RACE technology to amplify the 5' and 3' ends, splicing to obtain a banana MADS-box gene, and design gene 5' and 3' end primers according to the splicing results to Banana fruit total RNA is reverse-transcribed cDNA as a template, and amplified. The nucleotide sequence of the PCR product obtained is sequence 1 in the sequence table. The gene shown in the nucleotide sequence is named MuMADS2, and the protein encoded by it is MuMADS2 , the amino acid sequence of the protein is sequence 2 in the sequence list.

[0051] 2. Expression analysis of banana MuMADS2

[0052] Total RNA extraction and cDNA reverse transcription were perfo...

Embodiment 2

[0067] Embodiment 2, the functional research of transmutation MuMADS2 tomato

[0068] 1. Phenotype of MuMADS2 tomato

[0069] Sow the T2-generation MuMADS2 tomato (MuMADS2), the T2-generation empty vector tomato and wild-type tomato (control) numbered L12, L13, L15, L26, L27, L53, L56, and sow the tomato seeds of the above lines in the seedling pots. At 25°C, light 3000lux, 8h / d, cultivate for about 2 months. When the plant grows robustly and the height of the plant is 15-20cm, the seedlings are transferred to the field. There were 20 strains for each strain, and the experiment was repeated three times, and the results were average ± standard deviation.

[0070] The result is as Figure 3-Figure 5 shown, where image 3 For morphological observation, Figure 4 For the regulation of fruit shape and size by MuMADS2, Figure 5 It is the regulation of fertility by MuMADS2;

[0071] image 3 It can be seen from the results that the MuMADS2 tomato plants of the T2 generation s...

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Abstract

The invention provides an application of MuMADS2 to culturing of a fruit quality-improved transgenic plant. The invention provides an application of a MuMADS2 protein or an encoding gene of the MuMADS2 protein to regulation and control of a plant phenotype, improvement on the plant fruit quality and / or increase in a gene expression quantity which is relevant to fruit maturity; and the amino acid sequence of the MuMADS2 protein is a sequence 2 in a sequence table. As proved by the results of reduction in the plant height and reduction in the quantity of fruit seeds in an experiment in which the MuMADS2 is introduced into a tomato for obtaining seven independent strains of a MuMADS2 transgenic tomato driven by a 35S promoter and the MuMADS2 is excessively expressed, the MuMADS2 plays an important role in the processes of fruit development and quality forming.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an application of MuMADS2 in cultivating transgenic plants with improved fruit quality. Background technique [0002] The protein encoded by the MADS-box gene is a large number of transcription factor families that are very conservative in evolution. The first MADS-box gene isolated in plants is DEFICENS (DEF) isolated from snapdragon grass and DEFICENS (DEF) from Arabidopsis thaliana The AGAMSUS (AG) gene isolated from the plant, and through continuous research on these two model plants, it was found that the MADS-box gene plays an important role in the development of flowers, regulating the plant from flowering time to the formation of flower organs. , pollen development and fertility, etc. [0003] The fruit ripening process is a very complex change process, including a series of changes in physiological and biochemical processes, such as changes in cell wall structure, changes i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63A01H5/00
Inventor 徐碧玉金志强刘菊华贾彩红张建斌王甲水李羽佳谭光兰
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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