Apophysomycesspartina as well as acid-producing fermentation method and application thereof
A kind of salt marsh squamous mold and salt marsh technology, applied in the field of microbial engineering, can solve the problem of weakened phosphorus dissolving ability, achieve good phosphorus dissolving effect, save power and labor costs
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[0089] 1) Preparation of fermentation broth and measurement of pH value
[0090] Improved Martin's liquid medium: 1.0 g of dipotassium hydrogen phosphate, 0.5 g of magnesium sulfate, 10 g of glucose, 5 g of peptone, 13 g of sodium chloride, and 1000 ml of water. The culture conditions are: inoculum size 5%, solution volume 150mL / 250mL, temperature 28°C, rotation speed 160rpm. Each of the experimental group and the negative control group had three repetitions, and the pH value was measured every 24 hours.
[0091] 2) Qualitative and quantitative analysis of organic acids
[0092] Solution configuration
[0093] 10% sulfuric acid methanol solution: put sulfuric acid and methanol in the refrigerator for 1 hour, under ice-cooling and stirring conditions, slowly add 50ml of concentrated sulfuric acid into 450ml of methanol and shake well.
[0094] Benzoic acid internal standard solution: Accurately weigh 2g of benzoic acid and add 20ml of methanol.
[0095] Standard solution: A...
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