Apophysomycesspartina as well as acid-producing fermentation method and application thereof

A kind of salt marsh squamous mold and salt marsh technology, applied in the field of microbial engineering, can solve the problem of weakened phosphorus dissolving ability, achieve good phosphorus dissolving effect, save power and labor costs

Inactive Publication Date: 2013-10-02
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it was previously reported that the phosphorus-solubilizing fungi are generally stronger than bacteria, and the phosphorus-solubilizing ability of bacteria is weakened during further separation and purification, and fungi can still maintain a high phosphorus-solubilizing activity.

Method used

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  • Apophysomycesspartina as well as acid-producing fermentation method and application thereof
  • Apophysomycesspartina as well as acid-producing fermentation method and application thereof
  • Apophysomycesspartina as well as acid-producing fermentation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0089] 1) Preparation of fermentation broth and measurement of pH value

[0090] Improved Martin's liquid medium: 1.0 g of dipotassium hydrogen phosphate, 0.5 g of magnesium sulfate, 10 g of glucose, 5 g of peptone, 13 g of sodium chloride, and 1000 ml of water. The culture conditions are: inoculum size 5%, solution volume 150mL / 250mL, temperature 28°C, rotation speed 160rpm. Each of the experimental group and the negative control group had three repetitions, and the pH value was measured every 24 hours.

[0091] 2) Qualitative and quantitative analysis of organic acids

[0092] Solution configuration

[0093] 10% sulfuric acid methanol solution: put sulfuric acid and methanol in the refrigerator for 1 hour, under ice-cooling and stirring conditions, slowly add 50ml of concentrated sulfuric acid into 450ml of methanol and shake well.

[0094] Benzoic acid internal standard solution: Accurately weigh 2g of benzoic acid and add 20ml of methanol.

[0095] Standard solution: A...

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Abstract

The invention belongs to the technical field of microorganism engineering, and in particular relates to apophysomycesspartina as well as an acid-producing fermentation method and application thereof. The apophysomycesspartina SM-1 is separated and purified from marine salina, and is identified, through morphology and molecular biology such as 18SrDNA, 28SrDNA, ITS and the like, to be a new species of mucoraceae apophysomyces, named as apophysomycesspartina; the apophysomycesspartina is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7717 on June 19, 2013. The apophysomycesspartina is applicable to saline soil improvement as well as cultivation and plantation of halophyte.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a salt marsh squamous mold and its fermentation acid production method and application. Background technique [0002] There are many types of microorganisms in soil that have the effect of dissolving phosphorus, including bacteria, actinomycetes and fungi. Among the phosphorus-solubilizing microorganisms reported so far, bacteria are the most diverse, and the number and types of phosphorus-solubilizing fungi are far less than those of phosphorus-solubilizing bacteria, mainly Penicillium ( Penicillium ), Aspergillus ( Aspergilhu ) and Rhizopus ( Rhizopus ). However, it was previously reported that the phosphorus-solubilizing fungi are generally stronger than bacteria, and the phosphorus-solubilizing ability of bacteria is weakened during further separation and purification, and fungi can still maintain a high phosphorus-solubilizing activity. Now, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/48C12P7/44C09K17/00A01G1/00C12R1/645C09K101/00
Inventor 钦佩李思宇张焕仕
Owner NANJING UNIV
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