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A high-efficiency phosphate-solubilizing Bacillus megaterium in the rhizosphere of forest trees and its application

A technology of Bacillus megaterium and Bacillus megaterium, applied in the field of microorganisms, can solve problems such as limited application effect

Active Publication Date: 2020-12-01
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still relatively few domestic studies on phosphorus-dissolving bacteria in the rhizosphere of forest red-yellow soil, and the selected ones all come from the soil of cultivated land crops, and the application effect in forestry production is limited.

Method used

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  • A high-efficiency phosphate-solubilizing Bacillus megaterium in the rhizosphere of forest trees and its application
  • A high-efficiency phosphate-solubilizing Bacillus megaterium in the rhizosphere of forest trees and its application
  • A high-efficiency phosphate-solubilizing Bacillus megaterium in the rhizosphere of forest trees and its application

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Embodiment Construction

[0022] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0023] 1. The strain origin, breeding and gene sequencing of Bacillus megaterium

[0024] Source of the strain: Bacillus megaterium L205 of the present invention is isolated and selected from the rhizosphere soil of forest trees in the red-yellow soil region of Guangxi, and the preservation information is the same as above.

[0025] The morphological characteristics of Bacillus megaterium L205 are: the color of the colony is white, the cells are rod-shaped, and there are spores.

[0026] The physiological and biochemical characteristics of Bacillus megaterium L205 are: Gram stain positive, catalase positive, oxidase positive.

[0027] Bacillus megaterium L205 was obtained through the following specific isolation steps:

[0028] A. Soil collection: Collect rhizosphere soil of trees with good growth in Wuzhou, Ningming, Luzhai, Tianlin and oth...

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Abstract

The invention discloses an efficient phosphate solubilizing bacillus megaterium in a tree rhizosphere. The preservation number is CCTCC NO. M 2017454, and the efficient phosphate solubilizing bacillusmegaterium is named as Bacillus megaterium L205. The test shows that the phosphate solubilizing amount of bacillus megaterium reaches 203.04mg / L, and the liquid bacterium can effectively solubilize insoluble inorganic phosphorus, increase the content of available phosphorus in red-yellow soil, and promote the growth of tree seedlings. The efficient phosphate solubilizing bacillus megaterium provided by the invention enriches the sink population of bacillus megaterium at the culture collection center and provides convenience for better exploitation of microbial population resources of bacillusmegaterium. Meanwhile, the bacillus megaterium strain L205 can secrete organic acids, solubilize inorganic phosphorus and improve the phosphorus absorption and utilization of plants, is especially suitable for the production of tree biological fertilizers and tree growth promoting bacteria in red-yellow soil areas in South China, and can be used for further developing compound microbial fertilizers and bacteria.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a high-efficiency phosphorus-dissolving bacillus megaterium in the rhizosphere of forest trees and its application. Background technique [0002] The rhizosphere is a place where soil-root system-microorganisms closely interact and interact, and it is also an important part of the soil micro-ecosystem. Sperber et al. found that most of the phosphorus-dissolving microorganisms appeared in the rhizosphere soil of plants, and the rhizosphere phosphorus-dissolving bacteria and the root system had a mutual promotion effect. Most of the soil in China is in a state of phosphorus deficiency. More than 95% of the phosphorus in the soil in southern China is insoluble phosphorus and cannot be directly absorbed by plants. Phosphorus has become the main factor affecting plant growth. At the same time, most of the fast-growing and high-yielding forests in the south are arti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00C05F11/08C12R1/11
Inventor 邓小军唐健曹继钊宋贤冲王会利潘波覃其云覃祚玉石媛媛
Owner GUANGXI FORESTRY RES INST
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