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Method of extracting procyanidine from huckleberries

A proanthocyanidin and extraction solvent technology, which is applied in the field of extraction of effective plant components, can solve problems such as difficult to realize large-scale industrial production, low proanthocyanidin content, serious environmental pollution, etc., and achieve good social benefits, high extraction rate, and high degree of technology Effect

Active Publication Date: 2014-02-12
CHANGSHA NATUREWAY BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, phosphoric acid is used to adjust the pH, which seriously pollutes the environment; the obtained product is a mixture of anthocyanins and proanthocyanidins, and the content of proanthocyanidins is low; and this method is only a small-scale experiment in the laboratory, and it is difficult to realize large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 500kg of lingonberry powder that has been washed, dried, and crushed through an 80-mesh sieve (the content of proanthocyanidins is 0.85% after testing), add it to the negative pressure cavitation equipment, add 2000L of 60% ethanol, and adjust the pressure to -0.06MPa, The extraction temperature was 25°C, extracted for 30 minutes, filtered, and 2000L of 60% ethanol was added to the dregs for the second extraction, extracted for 30 minutes under the same conditions, filtered, and the two extracts were combined; Filter the organic membrane interception to obtain the retentate, discard the permeate; the retentate is then intercepted by an ultrafiltration organic membrane with a molecular weight cut-off of 100,000 to obtain the permeate; add the permeate to the pretreated 50kgAB-8 macroporous adsorption resin In the column, first eluted with deionized water until sugar-free, and then eluted with 8 times the column volume and 50% ethanol aqueous solution to obtain the el...

Embodiment 2

[0028] Take 500kg of lingonberry powder that has been washed, dried, and crushed through an 80-mesh sieve (the content of proanthocyanidins is 0.85% after testing), put it into a negative pressure cavitation apparatus, add 3000L of 80% ethanol, and adjust the pressure to -0.08MPa. Extract for 60 minutes at an extraction temperature of 40°C, filter, add 3000L of 80% ethanol to the dregs for the second extraction, extract for 60 minutes under the same conditions, extract at a temperature of 40°C, filter, and combine the two extracts; The ultrafiltration organic membrane with a molecular weight of 500 is intercepted to obtain the retained liquid, and the permeated liquid is discarded; the retained liquid is intercepted by an ultrafiltration organic membrane with a molecular weight cut off of 100,000 to obtain the permeated liquid; the permeated liquid is added to the pretreated 50kgAB- In the 8 macroporous adsorption resin column, first eluted with deionized water until sugar-free...

experiment example 1

[0031] Experimental Example 1 Detection of Proanthocyanidin Content

[0032] 1. Use Waters high performance liquid chromatography, chromatographic column C18 (4.6mm×250mm, 5um), mobile phase is V (acetonitrile): V (water) = 8:92, detection wavelength is 280nm, flow rate is 1.0mL / min , the injection volume is 60uL.

[0033] 2. The results are shown in Table 1:

[0034] Table 1 Content detection of cranberry proanthocyanidins

[0035]

[0036] As can be seen from the results: compared to the conventional extraction technology of lingonberry, the present invention has little energy consumption, short extraction cycle, significantly improved yield and recovery rate, and the extraction process is closely related to the following purification process, and the extraction technology of the present invention also makes the content obviously increase. The invention uses membrane technology to purify the lingonberry proanthocyanidins, the purity can reach more than 95%, an...

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PUM

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Abstract

The invention relates to extraction of effective components of plants, and specifically provides a production method of extracting procyanidine from huckleberries. The method comprises the following steps: crushing a huckleberry raw material; extracting by adopting a negative pressure cavitation method; filtering by using an ultrafiltration organic membrane; purifying by using resin to obtain a procyanidine product. The method provided by the invention can be used for increasing the yield of the procyanidine and reducing the environmental pollution, and facilitating the factory production and the reduction of the production cost.

Description

technical field [0001] The invention relates to the extraction of effective plant components, in particular to a method for extracting proanthocyanidins from lingonberries. Background technique [0002] Bilberry (Vaccinium spp), also known as Wenpu, Red Bean (Heilongjiang), Yapida (Daxinganling), is a deciduous shrub plant of Rhododendronaceae. It is praised as "Northern Red Bean". Tropical alpine regions. There are more than 130 kinds of lingonberries, mainly lingonberries, lingonberries, lingonberries, and cranberries. The lingonberry fruit is nearly round, mostly 0.5-2cm in diameter, blue-black or dark red. The main functions of lingonberry are: preventing blood vessel rupture, also known as a repairman of capillaries; protecting eyes; preventing cancer; improving chronic hepatitis B, etc. Juicing the lingonberries, making sugar buns, and making steamed buns are the main eating methods. [0003] Bilberry fruit is rich in nutrients, including anthocyanins, pectin, tann...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 李永旺谭家忠张飞达李芳
Owner CHANGSHA NATUREWAY BIOLOGICAL PROD
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